Fast crossbreeding method of pear tree
A technology of hybrid breeding, pear trees, applied in the field of rapid hybrid breeding of pear trees
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Embodiment 1
[0037] The experiment was carried out in the Pear Resource Garden of the Institute of Horticulture, Jiangsu Academy of Agricultural Sciences (Nanjing City). A total of 72 varieties such as Zaosu (see Table 1) (all are known public varieties). The female parent is Cui Guan and the male parent is Huang Guan.
[0038] (2) Test method
[0039] Spray 3 to 5 degrees of lime sulfur once on the pear tree before the buds germinate.
[0040] 72 varieties such as Zaosu pear tree were isolated by insect-proof nets at the early stage of budding (specifications: 20 mesh, wire diameter 0.18mm) ( figure 2 ), after the flowers wither, release the insect net to investigate the fruit setting rate. As a self-compatibility survey test among different varieties.
[0041] As the male parent required for hybridization, when the flower buds germinated, the flower bud branches were collected in the field and placed in a 25°C incubator to artificially stimulate the flowers, and the pollen was col...
Embodiment 2
[0050] 2. Picking: When picking, put diseased fruit, injured fruit and good fruit completely separately.
[0051] 3. Storage: Store the fruit in a ventilated and cool place. Generally, the shelf life of good fruit at room temperature is 8-15 days.
[0052] 4. Seed picking and cleaning: When the fruit softens, the seeds can be picked. Rinse the seeds with clean water and knead them with gauze to wash off the sticky matter on the surface of the seeds. Select full seeds, then put the seeds in a cool place to dry in the shade, and finally pack the dried seeds Put it into kraft paper and make a good label.
[0053] 5. seed sand storage
[0054] (1) Disinfection: soak the seeds for 2 hours before sand storage, and then soak them in 500-fold dilution of 80% carbendazim wettable powder fungicide for 2 to 3 seconds; use a pressure cooker to sterilize the river sand.
[0055] (2) Time: It is advisable to start sand storage in the middle and late December, and generally store in sa...
Embodiment 3
[0066] Select six groups of hybrid combinations, they are: Combination 1: Xizigreen×Cuiguan, Combination 2: Fengshui×Qingxiang, Combination 3: Cuiguan×Xizigreen, Combination 4: Love Ganshui×Qingxiang, Combination 5: Huasu × Cuiguan, combination six: Huangguan × Jinxing, a total of 6 combinations of 975 plants. In 2004, it was sown and cultivated in the pear resource garden of the Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, with a row spacing of 0.5×4 meters. In the cultivation of hybrids, except for the fixed stem of 60-70 cm in the second spring, the rest are almost not pruned, and they are allowed to grow naturally. Other fertilizer management is the same as that of the production garden.
[0067] The fruit bearing height is represented by the total length of the branches from the fruit to the root neck, and those less than 10 centimeters are rounded up, and the shortest length of each individual plant is the minimum height of the first fruiting of ...
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