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Rare earth-doped up-conversion nanoparticles for therapeutic and diagnostic applications

A nanoparticle, rare earth doping technology, applied in gene therapy, preparations for in vivo experiments, photodissociation of drugs in vivo, etc., can solve problems such as the inability to effectively produce singlet oxygen

Inactive Publication Date: 2012-11-14
SIRNAOMICS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In order to treat tumors, especially tumors in the deep part of the body, radiation sources with a wavelength of less than 700nm cannot be directly used as a radiation source to excite photosensitizers, because photosensitizers are located near tumors, and most tissue chromophores can easily absorb visible light, resulting in ineffective generation of photosensitizers. singlet oxygen

Method used

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  • Rare earth-doped up-conversion nanoparticles for therapeutic and diagnostic applications
  • Rare earth-doped up-conversion nanoparticles for therapeutic and diagnostic applications
  • Rare earth-doped up-conversion nanoparticles for therapeutic and diagnostic applications

Examples

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Embodiment Construction

[0114]Cell transfection experiments were performed using rare-earth-doped UCNPs coated with silica shells containing photosensitizers and modified with functional molecules such as TSDT on the particle surface. Mix a specific amount of surface-modified UCNP and Cy3-labeled siRNA in the required ratio to obtain a siRNA-UCNP mixture, and test the transfection effect of this new transfection system in vitro. The results are as follows: Figure 8 As shown, the transfection of HEK293 and HepG2 cell lines was observed under a fluorescence microscope, and very effective transfection results can be seen.

[0115] Next, the gene silencing effect of this introduced system was tested. The PC-3 cell line was used to set up control experiments under different conditions to compare the efficiency of gene silencing. The results are as follows Figure 9 shown. It can be seen that when siRNA-UCNP transfected PC-3 cells and stimulated with IR, the gene silencing effect was the best. Figure ...

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Abstract

This invention provides a composition matter comprising rare earth-doped up-conversion nanoparticles (UCNPs) encapsulated with a silica shell. In one embodiment, a photosensitizer is incorporated into the silica shell. In another embodiment, the composition further comprises a targeting molecule. In still another embodiment, a small interfering RNA (siRNA) molecule is also attached to the silica shell with the targeting molecule. The invention further provides methods for synthesizing such compositions and for using them in therapeutic and diagnostic applications. These applications use infrared or near infrared activation to excite the UCNPs.

Description

[0001] Cross references related to this patent application [0002] This application claims priority to US Provisional Patent Application No. 61 / 263,392, filed November 22, 2009, which is hereby incorporated by reference in its entirety. field of invention [0003] The present invention relates to a composition comprising rare earth-doped upconverting nanoparticles (UCNPs) encapsulated by a silicon shell, which enhances the release of chemical drugs, small interfering RNA (siRNA) inhibitors or polypeptide drugs ; The present invention also provides a method for synthesizing this nanoparticle, and the application of this composition in the treatment of various human diseases. One example of this is the use of siRNA inhibitors to treat cancer with the assistance of UCNP. Infrared light excites the upconversion nanoparticles to emit visible light, thereby activating the photosensitizer attached to UCNPs to generate singlet oxygen, destroying endosomal membranes, promoting the in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G02F1/35G06K7/10
CPCC12N2320/32C12N2310/14A61K49/003A61K49/0065A61K41/0057G01N33/587C12N15/111Y10T436/143333A61K41/0042G01N33/582
Inventor 王强帕特里克·Y·陆哈利·宏钧·杨
Owner SIRNAOMICS INC
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