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Degumming method through immobilized phospholipase A2

A technology of immobilizing phospholipase and degumming, which is applied in the direction of fat production, fat oil/fat refining, etc., to achieve the effects of cost reduction, good enzyme reusability, and phosphorus content reduction

Inactive Publication Date: 2012-11-14
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention is in order to solve the unreported problem that sodium alginate-chitosan composite carrier immobilized Rohalase MPL is used for degumming in the enzymatic degumming process, and proposes to immobilize Rohalase with sodium alginate-chitosan composite carrier MPL degumming method

Method used

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Effect test

specific Embodiment approach 1

[0009] Embodiment one: the method for degumming Rohalase MPL with sodium alginate-chitosan composite carrier immobilization is realized through the following steps: one, sodium alginate-chitosan composite carrier immobilization Rohalase MPL is used for hydration degumming oil degumming: Heat the hydrated degummed soybean oil to 85°C, add about 0.1% of 45% citric acid solution for acid reaction and cool down to a certain temperature, add about 4% of NaOH solution to adjust the initial pH to a certain value, and add hot soft water inside Appropriate amount, then add a certain amount of immobilized Rohalase MPL to carry out enzyme reaction for a certain period of time, the obtained degummed oil is centrifuged at 4000rpm for 20min, and then the upper layer oil sample is preserved, that is, the enzymatic degummed oil. 2. Sodium alginate-chitosan composite carrier immobilized Rohalase MPL recovery: the sodium alginate-chitosan composite carrier immobilized Rohalase MPL after the degu...

specific Embodiment approach 2

[0010] Embodiment 2: The difference between this embodiment and Embodiment 1 is that in step 4, the degumming temperature is 45° C. to 55° C. and the sodium alginate-chitosan composite carrier is used to immobilize Rohalase MPL to degumming the hydration degumming oil. Other steps are the same as in the first embodiment.

specific Embodiment approach 3

[0011] Specific embodiment three: the difference between this embodiment and specific embodiment one is that in step four, adjust the pH with about 4% NaOH solution to be 4.5 ~ 5.5 and then use sodium alginate-chitosan composite carrier to immobilize Rohalase MPL to water Chemical degumming oil degumming. Other steps are the same as in the first embodiment.

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Abstract

The invention provides a degumming method of hydrated degummed soybean oil through immobilized phospholipase A2. Influences of various degumming conditions such as degumming temperature, degumming initial pH, additive amount of the immobilized phospholipase A2 and deguming reaction time on soybean oil degumming effects are researched, and a response surface analytical method is adopted to optimize the researched factors. According to the method, the activity and stability of an enzyme in a reaction system are improved, the oil and fat refining cost is greatly reduced; additionally, the immobilized phospholipase A2 is adopted to degum the soybean oil, the phosphorus content of the finally obtained degummed soybean oil through an enzymic method can be reduced to 9.83 ppm, the activity of the immobilized enzyme can be maintained above 54% after reused for seven times, and significance is provided for the oil and fat refining.

Description

technical field [0001] The invention relates to a sodium alginate-chitosan composite carrier immobilized phospholipase A 2 Applied to the degumming method. Background technique [0002] Natural soybean oil needs to go through a series of refining processes to get the final oil product. The first step of refining is the degumming process, that is, the removal of phospholipids. The phospholipids are divided into hydratable phospholipids and non-hydratable phospholipids. Among them, hydratable phospholipids can be removed by hydration degumming; non-hydratable phospholipids Due to its remarkable hydrophobicity, it is difficult to remove it by conventional methods. In view of this characteristic, the enzymatic degumming of hydration degummed oil by phospholipase came into being. [0003] Now for enzymatic soybean oil degumming process, more phospholipase is used as phospholipase A 1 (PLA 1 ), PLA 1 Specific hydrolysis of natural phospholipid Sn-1 acyl group, to obtain Sn-2 ...

Claims

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Application Information

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IPC IPC(8): C11B3/00C11B3/04C11B3/06
Inventor 于殿宇江连洲胡立志李宝昌张佳宁邹小雨刘鑫李万振宋鹏王玥宋云花张春艳
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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