Paddy biofertilizer, preparation method thereof and bacillus subtilis
A technology of Bacillus subtilis and bio-fertilizer, applied in the field of agriculture, can solve the problems of not being able to prove the good growth of rice, not being able to improve the growth of rice, and increasing the root weight, so as to advance the rice tillering period, increase the number of rice tillers, and increase the root weight Effect
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Embodiment 1
[0035] Embodiment 1: Screening of Bacillus subtilis HY01 of the present invention
[0036] Take the commercially available wild-type Bacillus subtilis as the starting strain, culture it with a nutrient solution containing a mixture of rare earths at 30°C and 120r / min for 12 hours, then select a strong strain and repeat the above-mentioned cultivation steps 3-5 times, and then select a strong strain Bacterial strains are subjected to ordinary culture, when the number of bacteria reaches 10 7 cells / ml, treated under 500W microwave for 30-45s, screened the most robust strain, and named it HY01. Wherein, the nutrient solution is composed of 100 parts of beef extract, 1 part of peptone, 0.5 part of sodium chloride, and 0.15 part of rare earth mixture in parts by weight (gadolinium chloride: cerium nitrate weight ratio is 2:1), pH7. 2-7.4.
[0037] The above-screened strain HY01 was tested for its physiological and biochemical characteristics according to the method of "Common Bac...
Embodiment 2
[0038] Embodiment 2: the decomposing fermentation of poultry manure
[0039] Livestock manure: cow manure, deer manure, pig manure, chicken manure;
[0040] In terms of mass percentage, mix 70% poultry manure, 20% forage or straw powder, and 10% bran and place them in a fermentation tank. The height of the mixed material is 0.5-1.2 meters. When the temperature of the fermented material rises to 60 ° C, Start to turn the compost, and control the temperature not to exceed 70°C. The number of turns depends on the temperature of the fermented material. The fermentation process is generally completed in 4-6 days. The fermented material is fluffy, dark brown, and slightly fragrant or earthy.
Embodiment 3
[0041] Embodiment 3: prepare rice biological fertilizer of the present invention
[0042] Solid material composition: 40 parts of rice husk powder, 50 parts of wheat bran, 5 parts of soybean cake powder, 5 parts of corn flour, 0.05 part of magnesium sulfate and 0.01 part of manganese sulfate, the ratio of material to water is 1:1, tunnel microwave sterilization , adjust the pH value to 7.0-7.2 with alkaline substances such as calcium hydroxide;
[0043] Adding Bacillus subtilis with preservation number CGMCC No.6190 to the solid material and fermenting until the number of Bacillus subtilis is at least 1.2×10 10 per g, and the rate of Bacillus subtilis spores reaches at least 60%, and the rate of miscellaneous bacteria is less than 20%, to obtain fermented solid materials;
[0044] Take 5 parts of fermented solid material, 7.7 parts of humic acid, 0.3 part of agricultural rare earth (REO ≥ 40%), 50 parts of poultry manure of embodiment 2, silicon calcium magnesium fertilizer (...
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