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Method for automatically surveying and mapping growth curve of microorganism in food

A growth curve and microbial technology, which is applied in the preparation of test samples, measuring devices, and measuring electrical variables, etc., can solve the problems of expensive reagents, dependence on imports, and restrictions on the research level of food-borne pathogenic bacteria. Effects of stability, improved accuracy, and enhanced signal-to-noise ratio

Inactive Publication Date: 2012-10-24
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the system and supporting reagents are expensive and need to be imported, which seriously restricts the improvement of the research level of food-borne pathogens in my country.

Method used

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  • Method for automatically surveying and mapping growth curve of microorganism in food
  • Method for automatically surveying and mapping growth curve of microorganism in food
  • Method for automatically surveying and mapping growth curve of microorganism in food

Examples

Experimental program
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Effect test

Embodiment 1

[0041] Using the automatic surveying and mapping device of microbial growth curve in food and such as figure 2 The method shown for 10 5 Growth process curve of E. coli in cfu / mL (see Figure 4 Curve A, Figure 4 Curve B is a blank reference) for real-time mapping and growth curves obtained by plate counting (see Figure 4 Curve C) for comparison. from Figure 4 It can be seen that in section ab, Escherichia coli is in the lagging phase, and does not divide immediately for a period of time, the number of Escherichia coli remains constant, or increases very little, and the properties of the medium do not change significantly, so the frequency shift does not change significantly; The bc stage is the exponential growth period of Escherichia coli. The physiological metabolism of Escherichia coli converts carbohydrates, lipids and proteins in the medium into electroactive substances, which significantly changes the properties of the medium. The piezoelectric bulk acoustic wave...

Embodiment 2

[0043] The typical growth curves of fresh milk samples with different initial concentrations of bacteria were measured by an automatic surveying device for microbial growth curves in food. The results are as follows: Figure 5 shown. It can be seen from the figure that the greater the initial concentration of bacteria, the smaller the MDT, and conversely, the smaller the initial concentration of bacteria, the greater the MDT. The initial concentration of bacteria is about 10-10 6 At cfu / mL, there is a linear relationship between the logarithm of the initial bacterial concentration and the MDT, and the specific relationship is as follows:

[0044] LgC=7.216-0.432MDT (4)

[0045] In the formula, C is the initial concentration of microorganisms in the fresh milk sample, MDT is the detection time of microorganisms, and its correlation coefficient is about 0.96, indicating that there is a linear relationship between MDT and the logarithm of the initial concentration of bacteria i...

Embodiment 3

[0047] The microbial growth curve in rice noodles was surveyed and mapped in real time by using the automatic surveying and mapping device for microbial growth curve in food, the results can be found in Image 6 , Curve A is the growth curve of rice noodles after 121°C and 10min treatment, curves B and C are the growth curves of rice noodles after 80°C and 2min treatment, and curves D, E and F are the growth curves of microorganisms in untreated rice noodles Growth curve. from Image 6 It can be seen that there is no obvious frequency shift change in curve A, and there is no microbial detection time. It can be inferred that after the rice noodles are treated at 121 ° C for 10 minutes, the microorganisms in it can be completely inhibited; Curves B and C do not show significant changes until about 10 hours later. The change of frequency shift shows that the growth of microorganisms in rice flour is inhibited after 2min treatment at 80°C, and most of the microorganisms are kille...

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Abstract

The invention discloses a method for automatically surveying and mapping the growth curve of a microorganism in food. The method has the main contributions that a novel method that the microbiological analysis is carried out by surveying and mapping the growth curve of the microorganism is proposed starting with the growth rule of the microorganism in the food and is a technological innovation on the traditional analytical method; the condition that the growth information of the microorganism can be converted into measurable frequency information by a piezoelectric acoustic wave sensor is discovered through experimental study, and thus, a design principle for designing a fast and efficient novel surveying and mapping device is provided; and a device for automatically surveying and mapping the growth curve of the microorganism in the food is designed and constructed under the guidance of the novel method and the design principle. The device constructed according to the method has the advantages that the real-time and automatic surveying and mapping of the growth curve of the microorganism in the food can be realized, and the in-depth study on the microorganism in the food, such as the study of food processing methods, food shelf lives and microbial antimicrobial activity, can be carried out according to the obtained growth curve; and as the device has the characteristics of simplicity and convenience in operation, accuracy in result, and the like, the device is hopeful to be popularized and applied in microorganism-related research of food processing, storage and selling.

Description

technical field [0001] The invention relates to a method for automatic surveying and mapping of microbial growth curves in food. Background technique [0002] The globalization of world trade has also created food safety risks. In recent years, food safety incidents have occurred frequently around the world, such as: the "spinach poisoning incident" that infected the large intestine in the United States, the "salmonella incident" of peanut butter infected with Salmonella, the snow-printed milk poisoning caused by tens of thousands of people in Japan caused by Staphylococcus enterotoxin, the British mad cow disease in France, listeria infection in France, avian flu in Thailand, etc. There are billions of cases of foodborne diseases every year in the world, and the probability of foodborne diseases in developed countries is also quite high. The economic losses caused by billions of cases of foodborne illness, increased medical expenses, recall of unsafe food, and destruction...

Claims

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Application Information

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IPC IPC(8): G01R27/02G01R27/22G01N27/07G01N1/28
Inventor 任佳丽周逸阳马丽娜张慧李忠海林亲录
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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