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Kit for chemiluminescent quantitative immunoassay of free beta-human chorionic gonadotrophin (hCG) and preparation method thereof

A chorionic gonadotropin and chemiluminescence immunological technology, applied in the field of immunoassay medicine, can solve the problems of high cost and low sensitivity, and achieve the effects of low cost, high sensitivity and strong specificity

Active Publication Date: 2012-10-24
BIOSCIENCE (TIANJIN) DIAGNOSTIC TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The problem to be solved by the present invention is to provide a free β-hCG chemiluminescence quantitative detection kit and its preparation method, which solves the problems of low sensitivity and high cost

Method used

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  • Kit for chemiluminescent quantitative immunoassay of free beta-human chorionic gonadotrophin (hCG) and preparation method thereof
  • Kit for chemiluminescent quantitative immunoassay of free beta-human chorionic gonadotrophin (hCG) and preparation method thereof
  • Kit for chemiluminescent quantitative immunoassay of free beta-human chorionic gonadotrophin (hCG) and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Preparation of free β-human chorionic gonadotropin chemiluminescent immunoquantitative detection kit

[0033] Free β-human chorionic gonadotropin chemiluminescence immunoquantitative detection kit, including:

[0034] 96-well white microplate coated with free β-hCG antibody;

[0035] free β-hCG antibody-horseradish peroxidase (HRP) conjugate;

[0036] free β-hCG calibrator;

[0037] free β-hCG quality control;

[0038] Luminescent liquid A liquid and B liquid, A liquid contains luminol and p-iodophenol, B liquid contains carbamide peroxide;

[0039] 20 times concentrated solution, the formula is 75.5g / L Tris, 120g / L NaCl, 5mL / L Tween-20, 1g / LProclin300. Proclin300 is selected from American sigma company.

[0040] Prepare free β-human chorionic gonadotropin (free β-hCG) Chemiluminescent Immunoquantitative Detection Kit by the following method

[0041]1) Coating: Dilute the free β-hCG antibody with 0.02M pH7.4 phosphate buffer to 1~10ug / mL, add it to a wh...

Embodiment 2

[0069] Example 2: Preparation of free β-human chorionic gonadotropin chemiluminescent immunoquantitative detection kit

[0070] The kit of the present invention was prepared in the same manner as in Example 1, except that the solid phase carrier was a 48-well microwell plate.

Embodiment 3

[0071] Embodiment 3: the using method of kit of the present invention

[0072] (1) Equilibrate the test kit at room temperature (18-25°C) for 30 minutes.

[0073] (2) Preparation of lotion: dilute the concentrated lotion with distilled water at 1:20 (1mL of lotion plus 19mL of distilled water). If the concentrated lotion has crystals, place the concentrated lotion at room temperature or 37°C until the crystals dissolve before diluting.

[0074] (3) Preparation of luminous liquid: Take an appropriate amount of luminous liquid A and luminous liquid B and mix them in equal volumes 5 minutes before use.

[0075] (4) Take out an appropriate amount of coated slats according to the needs of the experiment. Set 1 well for blank control, 2 wells for calibrator, and 10 wells for quality control. Add 50 μL of free β-hCG calibrator, quality control substance and sample to each well, and do not add calibrator, quality control substance and sample to the blank control.

[0076] (5) Add ...

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Abstract

The invention discloses a kit for chemiluminescent quantitative immunoassay of a free beta-human chorionic gonadotrophin (hCG). The kit comprises a free beta-hCG antibody-coated plate, a free beta-hCG antibody enzyme conjugate, a free beta-hCG calibrator, a free beta-hCG quality control material, a washing liquid concentrated by 20 times, a light-emitting solution A and a light-emitting solution B. The kit has the advantages of high detection sensitivity, low cost and simple and fast operation, and has a high clinical application value. The invention also discloses a preparation method of the kit.

Description

technical field [0001] The invention belongs to the field of immunoanalysis medicine, in particular to a free β-hCG chemiluminescent immunoquantitative detection kit and a preparation method thereof, which can be used for quantitative determination of free β-human villi in human serum and plasma Membrane gonadotropin content. Background technique [0002] Human chorionic gonadotropin (hCG) is a glycoprotein hormone secreted by the trophoblast cells of the placenta. It consists of two non-covalently bonded glycoprotein subunits, subunit and β subunit. The subunits are shared by the anterior pituitary hormones, while the β subunit is unique to HCG. There will be no cross-reaction with FSH, LH and TSH, etc. Although the structure is similar to β-LH, the 24 amino acids at the end are not found in β-LH, and the β-subunit is specific to human chorionic gonadotropin of. Free β-human chorionic gonadotropin (free β-hCG) is the β subunit of HCG in an unbound state, which is of grea...

Claims

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Application Information

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IPC IPC(8): G01N33/76
Inventor 刘萍张影宋启超范利花
Owner BIOSCIENCE (TIANJIN) DIAGNOSTIC TECH CO LTD
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