Method for testing efficacy of duck hemorrhagic ovaritis inactivated vaccine
A technology for hemorrhagic oophoritis and inactivated vaccine, which is applied in the field of veterinary biological product inspection to achieve the effect of objective method and strong operability
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Embodiment 1
[0023] Example 1 Duck Hemorrhagic Ovariitis Inactivated Vaccine Efficacy Testing Age in Days of Ducks
[0024] The Peking ducks aged 42, 50, 100, 120, 200, 300, 400, 560 and 600 days were divided into 9 groups, 10 in each group, and each group was Peking ducks of the same age (purchased from Beijing Jinxing Duck Co., Ltd. Center Nankou breeding duck farm, duck hemorrhagic ovariitis antibody was negative). The test ducks were challenged with the same dose of DHOV-HB strain virus by oral route, containing about 3×10 4 ELD 50 Duck blood was collected through the wing vein 5 days after challenge, and the serum was separated aseptically. Serum was inoculated with 6-day-old SPF chicken embryos via the yolk sac route, 5 eggs / duck, 0.2ml / embryo, and incubated at 37°C for 168 hours. Chicken embryos that died within 24 hours were discarded, and the number of chicken embryos that died within 24 to 168 hours and were positive for DHOV by RT-PCR was calculated. One or more of the 5 chi...
Embodiment 2
[0028] Embodiment 2 Duck hemorrhagic ovariitis inactivated vaccine efficacy evaluation with the sex of the duck
[0029] Eighty 120-day-old Peking ducks of equal male and female were challenged orally with the same dose of DHOV-HB strain virus, containing approximately 3×10 4 ELD 50 / Only. From 1 to 8 days after the challenge, the blood of ducks was collected through the wing vein, 10 ducks per day, and the serum was separated aseptically. Serum was inoculated with 6-day-old SPF chicken embryos via the yolk sac route, and 5 chicken embryos were inoculated with serum from each duck, 0.2ml / embryo, and incubated at 37°C for 168 hours. Chicken embryos that died within 24 hours were discarded, and the number of chicken embryos that died within 24 to 168 hours and were positive for DHOV was calculated. One or more of the 5 chicken embryos in each serum was dead and DHOV positive was recorded as the duck virus isolation positive. The positive rate of virus isolation in female Pek...
Embodiment 3
[0030] Example 3 Immune protection of duck hemorrhagic ovariitis inactivated vaccine efficacy evaluation-virus isolation method (1)
[0031]Use the inactivated duck hemorrhagic ovariitis vaccine (batch number 2012001, developed by the Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences and Ruipu (Baoding) Biopharmaceutical Co., Ltd.) to immunize 10 140-day-old Peking ducks, all female, as a vaccine immunization Group. The immunization dose and method of the vaccine immunization group are as follows: the first immunization vaccine dose is 1ml / bird, and the chest muscle is injected. Two weeks after the first immunization, follow the same dose and the same way for the second immunization, and challenge the virus 4 weeks after the second immunization. The challenged virus strain is DHOV-HB strain, and the challenged virus is administered orally. The challenged dose for each duck is about 3×10 4 ELD 50 . At the same time, B...
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