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Lactic acid bacteria with effect of uric acid level reducing in blood

A technology of lactic acid bacteria and purine body, applied in the field of food or medicine for preventing and/or treating hyperuricemia

Active Publication Date: 2012-10-24
MEIJI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the lactic acid bacteria with the ability to decompose purine bodies reported in the above documents: Lactobacillus fermentum and Lactobacillus pentosus have the ability to produce gas, and cannot be said to be suitable from the viewpoint of application to food and beverages and pharmaceuticals. suitable strain

Method used

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  • Lactic acid bacteria with effect of uric acid level reducing in blood
  • Lactic acid bacteria with effect of uric acid level reducing in blood
  • Lactic acid bacteria with effect of uric acid level reducing in blood

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] [Example 1] About the in vitro experimental method of the uric acid reduction effect of lactic acid bacteria

[0061] The following method was used to study whether various lactic acid bacteria have the ability to decompose purine bodies.

[0062] Various lactic acid bacteria (cells) were placed in a closed container together with an oxygen adsorbent (AnaeroPack) (manufactured by Mitsubishi Gas Co., Ltd.) using Difco Lactobacilli MRS Broth (manufactured by BD) medium, and cultured anaerobically overnight at a temperature of 37°C. The cultured bacterial cell suspension was centrifuged at a speed of 3000 rpm and a temperature of 4° C. for 10 minutes, and the bacterial cell was recovered (collected) by sedimentation.

[0063] Using the above bacteria, prepare 1 × 10 with 0.1M sodium phosphate buffer 9 CFU / mL bacterial suspension.

[0064] After the various bacterial suspensions were prepared, inosine and guanosine were added to the various bacterial suspensions to reach ...

Embodiment 2

[0078] [Example 2] Experimental method in vivo about the uric acid-lowering effect of lactic acid bacteria

[0079] According to the method described in the existing literature (Non-Patent Literature 1), an animal model of dietary hyperuricemia was produced, and the influence of microorganisms (lactic acid bacteria) on the serum uric acid level of the animal was studied. The above method is specifically the following method: prepare a mixed feed containing 2.5% by weight of potassium oxonate and 1.0% by weight of RNA, feed it to rats, and compare the blood uric acid values ​​in the negative group and the control group after ingestion. After oral administration of allopurinol, an inhibitor of uric acid production, to the animal model of this method, it was found that the blood uric acid level of the animal model was significantly suppressed (Food Function Research New (No. 14), March 9, 2005 Nikko, Co., Ltd. Melcian Cleantec Environmental Research Center, http: / / www.m-cleantec....

Embodiment 3

[0120] [Example 3] Production of fermented milk

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Abstract

The present invention relates to lactic acid bacteria with an effect of uric acid level reducing in blood. According to the present invention, various lactic acid bacteria are cultured in the presence of a purine; a consumption amount of the purine and a production amount of purine decomposition products are determined; multiple lactic acid bacteria with significant purine decomposing abilities are selected; the selected lactic acid bacteria are adopted to orally feed rats, general statuses and serum uric acid levels of the rats are determined, and influence on the serum uric acid level due to the lactic acid bacteria feeding is researched, wherein the selected lactic acid bacteria are judged as the lactic acid bacteria having high purine decomposing abilities, and the rats are fed with a purine-containing feed; and two lactic acid bacteria capable of significantly inhibiting serum uric acid level increasing are found, wherein the two lactic acid bacteria are Lactobacillus gasseri OLL2959 and Lactobacillus oris OLL2779.

Description

[0001] This application is a divisional application with the Chinese application number 200780102278.8 with the filing date of November 29, 2007, and the title of the invention is "Lactic Acid Bacteria with the Effect of Lowering Blood Uric Acid Value". technical field [0002] The present invention relates to lactic acid bacteria capable of reducing blood uric acid value and its utilization method, and to food or medicine containing lactic acid bacteria for preventing and / or treating hyperuricemia. Background technique [0003] Hyperuricemia is a state in which there is excess uric acid in the blood due to decreased excretion of uric acid or excessive production of uric acid under the influence of environmental factors (lifestyle habits) and genetic factors. Although hyperuricemia sometimes has no subjective symptoms, it can also cause serious complications such as gout, renal dysfunction, urinary calculus, and arteriosclerosis. Gout is a typical complication of hyperuricem...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L1/30A61K35/74A61P19/06C12R1/225A61K35/747
Inventor 坪井洋金子纪子佐藤秋菜土屋义信
Owner MEIJI CO LTD
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