Preservation method for corneal limbus tissue
A preservation method and limbal technology, applied in the preservation, application, and animal husbandry of human or animal bodies, can solve the problems of losing epithelium, restricting the clinical application of limbal active tissue, and damaging the basement membrane, so as to achieve complete preservation and high The effect of activity and simple production process
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Embodiment 1
[0045] The preparation method of the present invention includes the following steps: the remaining limbal tissue of the corpse-derived eyeball after corneal transplantation is cleaned with sterile normal saline in a sterile and clean environment, and the blood stains on the surface are cleaned and disinfected. Fresh corneal limbus tissues are soaked and rinsed with antibiotic-containing phosphate buffer for 5-10min, rinse at least 3 times, each time greater than or equal to 5min; the surgeon wears sterile gloves with both hands and uses microscopic instruments to remove the conjunctival iris For tissue, place the sterile transwell chamber in a sterile cell culture dish with a diameter of 35mm, and spread the limbal tissue on the PVDF membrane of the transwell chamber; add the mid-term preservation solution to the bottom of the transwell chamber until the liquid surface is fully covered with the PVDF membrane Contact: Place the cell culture dish in the refrigerator to keep the am...
Embodiment 2
[0047] The preparation method of the present invention includes the following steps: the remaining limbal tissue of the corpse-derived eyeball after corneal transplantation is cleaned with sterile normal saline in a sterile and clean environment, and the blood stains on the surface are cleaned and disinfected. Fresh corneal limbus tissues are soaked and rinsed with antibiotic-containing phosphate buffer for 5-10min, rinse at least 3 times, each time greater than or equal to 5min; the surgeon wears sterile gloves with both hands and uses microscopic instruments to remove the conjunctival iris For tissue, place the sterile transwell chamber in a sterile cell culture dish with a diameter of 35mm, and spread the limbal tissue on the PVDF membrane of the transwell chamber; add the mid-term preservation solution to the bottom of the transwell chamber until the liquid surface is fully covered with the PVDF membrane Contact: Place the cell culture dish in a thermostat, keep the ambient ...
Embodiment 3
[0049] The preparation method of the present invention includes the following steps: the remaining limbal tissue of the corpse-derived eyeball after corneal transplantation is cleaned with sterile normal saline in a sterile clean environment, and the blood stains on the surface are cleaned and disinfected. Fresh corneal limbus tissues are soaked and rinsed with antibiotic-containing phosphate buffer for 5-10min, rinse at least 3 times, each time greater than or equal to 5min; the surgeon wears sterile gloves with both hands and uses microscopic instruments to remove the conjunctival iris For tissue, place the sterile transwell chamber in a sterile cell culture dish with a diameter of 35mm, and spread the limbal tissue on the PVDF membrane of the transwell chamber; add SHEM medium to the bottom of the transwell chamber until the liquid surface is fully connected to the PVDF membrane Contact: Put the cell culture dish in the refrigerator to keep the ambient temperature constant at...
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