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Heavy metal adsorbent for extracorporeal perfusion method and preparation method of heavy metal absorbent

An in vitro perfusion and heavy metal technology is applied in the field of biomedical materials to achieve the effects of large adsorption capacity, low preparation cost and treatment of heavy metal poisoning

Inactive Publication Date: 2012-10-10
汪志友
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to propose a heavy metal adsorbent for extracorporeal perfusion and a preparation method thereof, aiming at many problems in current methods of eliminating heavy metals in the human body and treating heavy metal poisoning

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Measure 20ml (sedimentation volume) of Sepharose FF medium microspheres stored in 20% ethanol aqueous solution, wash repeatedly with excess deionized water to remove ethanol; place the washed gel in a G3 sintered glass funnel Vacuum-dry 5min; Back successively with each 40ml of dimethyl sulfoxide aqueous solution of 20%, 50%, 70% clean the medium after the suction filtration; Then, add a certain amount of dimethyl sulfoxide (30ml) successively in the treated medium %), dichloroethane, epichlorohydrin (10%), sodium hydroxide (0.8M), water; the medium suspension was reacted at 40° C. for 2 hours under shaking conditions. After the reaction, it was repeatedly washed with excess deionized water until there was no residual epoxy group in the cleaning solution, and epichlorohydrin-activated agarose gel microspheres were obtained, and the agarose epoxy modification density was 60 μmol / ml gel.

Embodiment 2

[0018] Mix 8.5 volumes of 0.2M K2HPO4 solution with 1.5 volumes of 0.2M KH2PO4 solution to make the coupling buffer. Take 5 g of the epichlorohydrin-activated sepharose microspheres in Example 1 and suspend them in 10 ml of coupling buffer. Take a certain amount of metallothionein and dissolve it in the coupling buffer solution to completely dissolve it. Mix the gel suspension and the coupling buffer solution at a ratio of 1:0.5 to 1:1 for suspension reaction, seal in a water bath at 20°C-45°C, and keep stirring (or vibrating, shaking) for 20 hours. Wash with coupling buffer to remove excess ligand. Suspend the reacted and washed gel in a solution of 1 mol / L ethanolamine (pH 8.0), and react at 40°C-50°C for a minimum of 4 hours or overnight at room temperature to eliminate residual active groups on the surface of the medium. After the reaction, wash alternately with 0.1M acetate buffer (pH 4.0) containing 0.5M NaCl and coupling buffer (pH 8.3) containing 0.5M NaCl, and repea...

Embodiment 3

[0020] Measure 20ml (sedimentation volume) of Sepharose FF medium microspheres stored in 20% ethanol aqueous solution, wash repeatedly with excess deionized water to remove ethanol; place the washed gel in a G3 sintered glass funnel Vacuum dry for 5 minutes; then wash the medium after suction and filtration with 5 times the volume of 30% and 70% acetone aqueous solution; finally wash 5 times with 5 times the volume of 100% dioxane, drain and transfer to a 200ml flask ; Add a certain amount of allyl glycidyl ether and boron trifluoride-ether to the treated medium in turn; react the medium suspension at 35°C for 1-2 hours under shaking conditions. After the reaction, the medium was washed with 5 times the volume of 30% and 70% acetone aqueous solutions in sequence, and then repeatedly washed with excess deionized water until there was no residual epoxy group in the cleaning solution, and allyl glycidyl ether activated agarose gel was obtained. Microspheres, allyl modification de...

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PUM

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Abstract

The invention discloses an adsorbent for removing heavy metals through extracorporeal blood perfusion and a preparation method of the absorbent. Activating agents are used activating resin microsphere carriers with good biocompatibility, then sulihydryl reagents are fixed through bonding or coupling to form the heavy metal adsorbent, the surface of the heavy metal adsorbent is provided with a great quantity of sulihydryl functional groups and an adsorption effect through complexing or chelation between adsorption target liquid compositions and heavy metals and ions thereof is facilitated; the biocompatibility and the adsorption capacity are good; and the adsorbent has the characteristics of low preparation cost, simple synthesis process, strong adsorption capacity, rapid adsorption speed and the like, and can be used for extracorporeal whole-blood perfusion to remove in-vivo heavy metals and treat heavy metal poisoning.

Description

technical field [0001] The invention relates to a heavy metal adsorbent for extracorporeal perfusion and a preparation method thereof, belonging to the field of biomedical materials. Specifically, it relates to a method for extracting human blood, serum, plasma, plasma components, bone marrow fluid, cerebrospinal fluid, cell culture fluid, cell homogenization fluid, animal organ extract, plant extract or other Adsorption material for removing heavy metals and their ions by adsorption (complexation, chelation) in liquid components and a preparation method thereof. Background of the invention [0002] Human beings are in contact with or use air, soil, rivers, lakes, aquatic products, animals, plants, minerals polluted by various pollution sources such as industrial waste gas, waste water, waste residue, waste, automobile exhaust, pesticides, fertilizers, chemical reagents, additives, paints, etc. , Food, drinking water, domestic water, medicines, cosmetics, health care produc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/24B01J20/30B01D15/08
Inventor 汪志友
Owner 汪志友
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