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Preparation method of cow mammitis vaccines

A technology for dairy cow mastitis and vaccines, applied in microorganism-based methods, biochemical equipment and methods, medical preparations containing active ingredients, etc. Effect

Inactive Publication Date: 2012-10-10
INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, few vaccines have been successfully developed, most of which are the first-generation complete bacterial vaccines, and the effect is not obvious

Method used

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  • Preparation method of cow mammitis vaccines

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Effect test

specific Embodiment approach 1

[0022] Specific embodiment one: the preparation method of a kind of cow mastitis vaccine of the present embodiment is carried out according to the following steps:

[0023] 1. Preparation of Staphylococcus aureus toxin

[0024] a. Use an inoculation loop to pick Staphylococcus aureus, inoculate it in nutrient broth, and culture it in a 37°C incubator for 18-24 hours to obtain a culture solution; draw 10 μL of the culture solution and spread it evenly on the blood agar plate medium , after culturing in a 37°C incubator for 18-24 hours, pick a single colony with an inoculation loop diameter greater than 1 mm, inoculate it on a nutrient agar slant medium, and then place it in a 37°C incubator for 18-24 hours to obtain golden yellow grapes Coccus nutrient agar slant culture; pick an inoculation loop of Staphylococcus aureus nutrient agar slant culture, inoculate it in the toxin-producing medium, shake and culture it at 37°C for 18-24 hours, which is the seed solution; b. a The se...

specific Embodiment approach 2

[0036] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the operations from Step 1 to Step 6 are all performed under sterile conditions. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0037] Specific embodiment 3: This embodiment differs from specific embodiments 1 to 2 in that: the staphylococcus aureus toxin titer described in step 1 is 1024-4096. Others are the same as the specific embodiment 1 to 2.

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Abstract

A preparation method of cow mammitis vaccines relates to a preparation method of vaccines. The method comprises preparing staphylococcus aureus toxin with the titer of more than 1024, staphylococcus aureus similar toxin with the titer of more than 128, staphylococcus aureus mycoprotein, streptococcus agalactiae vaccines and escherichia coli vaccines, and obtaining the cow mammitis vaccines after compatibility of medicines. The cow mammitis vaccines are safe and free of toxin, and protection rate on a mouse in a toxin studying group achieves 91.7%. The preparation method is applied in the field of prevention and cure of cow mammitis.

Description

technical field [0001] The invention relates to a preparation method of a vaccine. Background technique [0002] Cow mastitis (Mastitis) is the most common infectious disease in adult dairy cows. It is mainly an inflammation caused by microbial infection of the cow's mammary gland tissue. It mostly occurs during the postpartum lactation period. The disease exists widely in all parts of the world and is a common disease in dairy cows. , frequently-occurring diseases, is the most serious disease causing economic losses in the dairy industry. There are about 150 kinds of pathogenic microorganisms that cause mastitis in dairy cows, mainly Staphylococcus aureus, Escherichia coli, and Streptococcus agalactiae. These three bacteria cause more than 90% of the total incidence of mastitis in cows. [0003] The current treatment of cow mastitis is mainly antibiotics, which have been used for the treatment of cow mastitis for more than 50 years, and have played a certain role in the pr...

Claims

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Application Information

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IPC IPC(8): A61K39/108A61K39/09A61K39/085A61P31/04C12N1/20C12R1/19C12R1/46C12R1/445
Inventor 曲晓军孙建华夏海华于冲王金英李思明
Owner INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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