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Method for processing microbiological feed based on spirit vinasse and miscellaneous meal

A technology of microbial feed and processing method, which is applied in the field of microbial feed, can solve the problems of insignificant effect of probiotics in feed utilization, high cost, poor degradation effect, etc., and achieve obvious ecological and social benefits, high number of active probiotics, Obvious effect of feeding benefit

Active Publication Date: 2012-10-10
陈华友
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many researchers have separated rice husk from protein, etc., but in fact the process is complicated, costly, and wasteful, and it is not easy to promote
Invention patent driving device, light quantity adjusting device, and lens driving device (CN1285156A), method for producing green feed additives for livestock and poultry healthy breeding by using distiller's grains (CN101491290A), method for producing high-enzyme activity and high-protein feed additives by fermentation of waste distiller's grains (CN101366445A) , to dry part of the fresh distiller's grains, which consumes a lot of energy. It is inoculated with Geotrichum candidum or Paecilomyces for degradation, but the effect on lignin is not obvious, and the product has no probiotic effect
The invention patent (CN101919490A) mainly uses cellulase when degrading distiller's grains. Due to the lack of ligninase, the addition amount is limited, and the lignocellulose degradation of distiller's grains is also limited, and the cost is also higher. Moreover, there is only one step of yeast Fermented with Bacillus subtilis, there are no anaerobic probiotics, and the types of probiotics are incomplete
Invention patent A yeast protein feed based on distiller's grains and its production method (CN101785524A) only rely on yeast to use distiller's grains, and the degradation effect is not good
The above products and most of the biological feeds on the market have to go through drying treatment, the bacterial enzymes are seriously inactivated, the feed utilization rate and the effect of probiotics are not obvious, and the raw materials generally have to be sterilized, so the overall cost is high, which is not conducive to industrialization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for processing microbial feed based on distiller's grains and miscellaneous dregs, the main steps are as follows:

[0024] First, pretreatment: add food-grade Aspergillus and Neurospora to the first-stage fermentation medium made of distiller's grains to carry out the first-stage fermentation;

[0025] Neurospora and Aspergillus seed culture and production media.

[0026] Slant medium and plate medium: 200 g potato, 20 g sucrose, 20 g agar, in a constant volume of 1 L, pH 5.0, sterilized at 110°C for 30 min. Inoculate at 30°C and culture for 7-10 days.

[0027] Seed liquid medium: KH 2 PO 4 2g, MgSO 4 0.3g, CaCl 2 0.3g, peptone 5g, yeast extract 3g, wort 3g, FeSO 4 ·7H 2 O 0.005g, ZnSO 4 0.0014g, MnSO 4 .4H 2 O 0.0016g, CoCl 2 0.002 g, dilute to 1 L, and adjust the pH to 5.0. Take a 500 ml Erlenmeyer flask, add 100 ml of the prepared seed liquid medium into the Erlenmeyer flask, and sterilize at 110°C for 30 minutes.

[0028] Preparation o...

Embodiment 2

[0071] A method for processing microbial feed based on distiller's grains and miscellaneous dregs, the steps are as follows:

[0072] 1) Pretreatment: Add food-grade Aspergillus and Neurospora to the primary fermentation medium made of distiller's grains for primary fermentation.

[0073] Distiller's grains are used after being crushed to 20-100 mesh in a wet state. Aspergillus niger (Aspergillus niger) and Aspergillus oryzae (Aspergillus oryzae) are used;

[0074] The weight components of the first-stage fermentation medium are:

[0075] 90% fresh distiller's grains with a water content of 60%;

[0076] Ammonium nitrate 1%;

[0077] Ammonium sulfate 1%;

[0078] Ammonium chloride 1%;

[0079] Urea 3%;

[0080] Calcium carbonate 0.2%;

[0081] Potassium dihydrogen phosphate 3%;

[0082] Magnesium sulfate 0.3%;

[0083] Magnesium nitrate 0.3%;

[0084] Magnesium Chloride 0.2%.

[0085] To start the first-stage fermentation for the first time, it is necessary to make ...

Embodiment 3

[0099] A method for processing microbial feed based on distiller's grains and miscellaneous dregs, the steps are as follows:

[0100] 1) Pretreatment: Add food-grade Aspergillus and Neurospora to the primary fermentation medium made of distiller's grains for primary fermentation.

[0101] Distiller's grains are used after being crushed to 20-100 mesh in a wet state. Aspergillus niger ( Aspergillus niger ); Neurospora is Neurospora crassa ( Neurospora crassa ).

[0102] The weight components of the first-stage fermentation medium are:

[0103] Fresh distiller's grains with a water content of 55-70% 98%;

[0104] Ammonium nitrate 0.1%;

[0105] Urea 0.1%;

[0106] Calcium carbonate 0.1%;

[0107] Potassium dihydrogen phosphate 0.7%;

[0108] Magnesium Sulfate 1%.

[0109] To start the first-stage fermentation for the first time, it is necessary to make liquid mold seed liquid. The ratio of the number of viable bacteria of Aspergillus and Neurospora in the mold seed liq...

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Abstract

The invention relates to a method for processing microbiological feed based on spirit vinasse and miscellaneous meal. The method comprises the following steps: performing primary fermentation pretreatment on neurospora and aspergillus by using the spirit vinasse as a raw material; completely degrading lignin, cellulose and starch of the spirit vinasse; adding part of cottonseed meal; performing secondary fermentation on bacillus and saccharomycetes; absorbing nutrient substances generated by that mould degrades the waste and nutrient substances decomposed by the mould; adding bean pulp and rape seed meal; and inoculating lactic acid bacteria and bifidobacteria and performing tertiary anaerobic fermentation to prepare the finished product. In the method, solid raw materials are not sterilized at high temperature; water is not added or discharged in the fermentation process; and the finished product is not dried, and has the shelf life of more than one year, extremely high active probiotic quantity, abundant enzyme quantity, extremely few miscellaneous bacteria and obvious feeding benefit.

Description

technical field [0001] The invention relates to a feed, in particular to a microbial feed. Background technique [0002] At present, the output of distiller's grains in my country has reached more than 20 million tons, but because of its high water content, high acidity, easy mildew and inconvenient storage, the utilization rate is low. Distiller's grains are mostly dried and pulverized into feed, which has more fiber, insufficient crude protein, and extremely poor feeding effect. Since distiller's grains contain some starch (about 10%), lignocellulose (about 20-45%), crude protein (about 10-20%), and some organic components such as amino acids and organic acids, the nutrition is relatively rich. Effective utilization, turning waste into wealth, making up for the serious shortage of feed resources in our country, alleviating the contradiction between humans and animals competing for food, promoting the development of animal husbandry, and solving the pollution problem, ther...

Claims

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Application Information

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IPC IPC(8): A23K1/06A23K1/14A23K10/38
CPCY02P60/87
Inventor 陈华友杨胜利
Owner 陈华友
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