Preparation method for vessel intubation tube model of femoral artery and/or femoral vein of mouse

Abstract

The invention discloses a preparation method for a vessel intubation tube model of femoral artery and / or femoral vein of a mouse. The preparation method comprises the following steps: manufacturing a hose mould, manufacturing a J-shaped hose, performing a neck operation, performing a groin operation, mounting and fixing the J-shaped hose, and the like, specifically, bending a copper tube into a J shape; arranging a gap outside a bent part; after soaking a polyethylene conduit into hot sesame oil, immediately stretching, thereby obtaining a polyethylene conduit section; inserting the polyethylene conduit section into the hose mould; and soaking the bent part of the hose mould into water at 80 DEG C, and then cooling, and pulling out, thereby obtaining the J-shaped hose. The J-shaped hose is fixed at two parts of the groin and the neck of the mouse during an operation process, the motion range of the mouse is smaller, and the vessel intubation tube model cannot be separated when the mouse is clear-headed, so that the wide-source and low-cost mouse is taken as an experimental animal, the preparation method provided by the invention has the advantages that the changes in physiology, pathology and pharmacology of the mouse are continuously and dynamically observed and researched under the total clear-headed and free-acting states of the animal, the research parameter amplitude is small and the research result is better.

Description

technical field [0001] The invention relates to an animal intravascular cannulation model, in particular to a preparation method for a mouse femoral artery and / or femoral vein vascular cannulation model. Background technique [0002] The vascular catheterization model is an in vitro vascular model that needs to be established for the study of animal physiology, pathology, and pharmacology. It can dynamically and continuously observe and study the changes of various physiological and pathological indicators in the body in vitro, so as to master drugs, poisons, and nutrients. It is an economical and effective experimental research technology for the metabolism, physiological and pathological mechanism of substances such as drugs in vivo. At present, the vascular intubation model mainly uses large animals such as pigs, dogs and rats as model animals. , vein, caudal artery, vein and other blood vessels, such as the utility model with the publication number CN2626935, which dis...

AI Technical Summary

Problems solved by technology

However, most of the current vascular intubation models use large animals as experimental animals, with few sources and high cost, and because large animals exercise violently, interval research can only be carried out when the animals are not fully awake

Therefore, the currently established vascular catheterization models of this type of large animals cannot be used for continuous dynamic observation and research when the animals are fully awake and free to move. Physiological and pathological changes under

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