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Method for quickly screening and confirming multiple water-soluble alkaline pigments in food

A water-soluble and alkaline technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of inability to meet the requirements of non-detection and qualitative confirmation, not much pioneering research work, lack of confirmation technology, etc., to achieve shortening The detection process, the expansion of the scope of application, and the effect of improving the analysis efficiency

Active Publication Date: 2012-09-19
SHANGHAI ANPEL SCI INSTR +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the current national standards for the detection of food colorant content, most of them are detection methods for one colorant, and fast and accurate screening detection methods are rarely used. Even if there is detection for water-soluble alkaline colorants, it is limited to one colorant. However, there is a lack of methods for the simultaneous determination of multiple water-soluble alkaline colorants, the efficiency is low, and there are not many pioneering research works
Secondly, the detection methods are limited to TLC, GC, HPLC and spectrophotometry, etc., lack of supporting confirmation technology, cannot meet the requirements of non-detection and qualitative confirmation of banned additives, and the reliability is not high

Method used

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  • Method for quickly screening and confirming multiple water-soluble alkaline pigments in food
  • Method for quickly screening and confirming multiple water-soluble alkaline pigments in food
  • Method for quickly screening and confirming multiple water-soluble alkaline pigments in food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Sample preparation and determination methods of 15 kinds of water-soluble alkaline pigments in toffee:

[0041] 1. Extraction:

[0042]Weigh 1g of toffee (accurate to 0.01g) into a 50mL centrifuge tube, add 1mL of water, sonicate in a water bath at 60°C for 15min, add 5mL of water + n-hexane-saturated acetonitrile (2+3, volume ratio), vortex for 3min , ultrasonically extract in an ultrasonic water bath for 15min, centrifuge at 4000r / min for 10min, transfer the supernatant to a 50mL plastic centrifuge tube, and then extract the residue with 5mL of water + acetonitrile saturated with n-hexane (2+3, volume ratio) according to the above steps once. Combine the extracts, add 1g of sodium chloride, vortex and shake, let stand to separate layers, transfer and keep the upper organic phase, add 4mL of acetonitrile-saturated n-hexane to the lower aqueous phase, vortex for 3min, and centrifuge at 4000r / min After 10 min, the upper n-hexane phase was discarded. The retained upper...

Embodiment 2

[0066] Sample preparation and determination methods of 15 water-soluble alkaline pigments in syrup:

[0067] 1. Extraction:

[0068] Weigh 2g of syrup (accurate to 0.01g) into a 50mL centrifuge tube, add 2mL of water, sonicate in a water bath at 60°C for 15min, add 7mL of water + n-hexane-saturated acetonitrile (2+3, volume ratio), vortex for 3min, Ultrasonic extraction in an ultrasonic water bath for 15 minutes, centrifuge at 4000r / min for 10 minutes, transfer the supernatant to a 50mL plastic centrifuge tube, and extract the residue once with 7mL water + n-hexane saturated acetonitrile (2+3, volume ratio) according to the above steps . Combine the extracts, add 3g of sodium chloride, vortex and oscillate, let stand for stratification, transfer and keep the upper organic phase, add 6mL of acetonitrile-saturated n-hexane to the lower aqueous phase, vortex for 3min, and centrifuge at 4000r / min After 10 min, the upper n-hexane phase was discarded. The retained upper organic p...

Embodiment 3

[0076] Sample preparation and determination methods of 15 water-soluble alkaline pigments in chili sauce:

[0077] 1. Extraction:

[0078] Weigh 1g of chili sauce (accurate to 0.01g) into a 50mL centrifuge tube, add 2mL of water, ultrasonicate in a water bath at 60°C for 15min, add 6mL of water + n-hexane-saturated acetonitrile (2+3, volume ratio), vortex for 3min , ultrasonically extract in an ultrasonic water bath for 15min, centrifuge at 4000r / min for 10min, transfer the supernatant to a 50mL plastic centrifuge tube, and extract the residue with 6mL water + acetonitrile saturated with n-hexane (2+3, volume ratio) according to the above steps once. Combine the extracts, add 2g of sodium chloride, vortex and oscillate, let stand for stratification, transfer and keep the upper organic phase, add 5mL of acetonitrile-saturated n-hexane to the lower aqueous phase, vortex for 3min, and centrifuge at 4000r / min After 10 min, the upper n-hexane phase was discarded. The retained up...

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Abstract

The invention relates to the field of food detection, in particular to a method for quickly screening and confirming multiple water-soluble alkaline pigments in food. The method comprises the steps of extracting water-soluble alkaline pigments in different kinds of food by utilizing water and acetonitrile solution, purifying the water-soluble alkaline pigments by utilizing a polymer substrate cation exchange solid phase extraction column, and determining the water-soluble alkaline pigments by utilizing liquid chromatogram-mass spectrum / mass spectrum. Compared with traditional methods, solid phase extraction (SPE) is combined with liquid chromatographic-mass spectrometric (LC-MS) technology, the polymer substrate cation exchange solid phase extraction column is used for the purification treatment so as to simultaneously extract multiple water-soluble alkaline pigments in the food, the liquid chromatogram-mass spectrum / mass spectrum method is used for detecting the alkaline pigments, so that the application range of the method is enlarged, 15 kinds of water-soluble alkaline pigments can be quickly and accurately detected within a very short time, simpleness in operation is realized, the sensitivity is high, the detection flow can be greatly shortened, the analysis efficiency can be greatly improved, and quick screening and simultaneous confirmation can be realized.

Description

technical field [0001] The invention relates to the field of food testing, in particular to a rapid screening and confirmation method for various water-soluble alkaline pigments in food. Background technique [0002] At present, there are 23 categories of food additives in my country, and colorants belong to one of them. However, the standard method for testing food additives currently formulated by the state cannot detect and supervise many illegal additives. Many colorants can cause serious hazards even in trace amounts and are banned in the food industry. On the other hand, although some colorants are allowed to be added, the dosage must be strictly controlled. Colorants are divided into two categories: edible natural pigments and edible synthetic pigments. Most of the synthetic pigments are harmful to the human body. Some of their toxicity are directly toxic to the human body due to their chemical properties; Conduct a rigorous toxicological evaluation. Natural pigme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06
Inventor 伊雄海张晓梅邓晓军强维
Owner SHANGHAI ANPEL SCI INSTR
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