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Analysis method for quickly detecting urine creatinine by isotopic dilution, extractive electrospray ionization (EESI) and tandem mass spectrometry (MS/MS)

A technology of isotope dilution and tandem mass spectrometry, which is applied in the field of rapid detection of urine creatinine by isotope dilution electrospray extraction ionization tandem mass spectrometry. The effect of streamlining the analysis process

Active Publication Date: 2012-09-19
EAST CHINA UNIV OF TECH +1
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Problems solved by technology

However, the analysis of urine creatinine by LC-MS or GC-MS cannot meet the urgent needs of high-throughput sample analysis in clinical research of kidney diseases (such as early diagnosis of acute renal failure) due to the time-consuming steps of sample pretreatment and chromatography.

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  • Analysis method for quickly detecting urine creatinine by isotopic dilution, extractive electrospray ionization (EESI) and tandem mass spectrometry (MS/MS)
  • Analysis method for quickly detecting urine creatinine by isotopic dilution, extractive electrospray ionization (EESI) and tandem mass spectrometry (MS/MS)
  • Analysis method for quickly detecting urine creatinine by isotopic dilution, extractive electrospray ionization (EESI) and tandem mass spectrometry (MS/MS)

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Embodiment Construction

[0021] An analytical method for isotope dilution electrospray extraction ionization tandem mass spectrometry to rapidly detect urine creatinine. The EESI ionization source used is the electrospray extraction ionization source manufactured by the Jiangxi Provincial Key Laboratory of Mass Spectrometry Science and Instruments, East China University of Technology (US Patent No.: US 2008 / 0179511 A1), it has been widely used due to its simple construction. The mass spectrometer is the LTQ-XL linear ion trap mass spectrometer of Finnigan Company of the United States; the data processing system is the Xcalibur data processing system of Finnigan Company of the United States, and the specific steps are as follows:

[0022] (1) Preparation of creatinine and deuterated creatinine mother liquor: Accurately weigh 10.0 mg creatinine or deuterated creatinine solid standard sample, put it into a 100-mL volumetric flask, add high-purity water (resistivity 18.2 MΩ cm) to dissolve, and obtain a co...

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Abstract

The invention relates to an analysis method for quickly detecting urine creatinine by isotopic dilution, extractive electrospray ionization (EESI) and tandem mass spectrometry (MS / MS), and belongs to an analysis and detection means for a renal function biomarker. The method is characterized in that the detection means is that an EESI source is subjected to direct MS and MS / MS under normal pressure, linear trap quadrupole (LTQ)-MS / MS is set to be in a positive ion detection mode, the parameters of the EESI source, such as the composition of an electrospray solvent are adjusted, an isotopic dilution technology and a standard addition method are adopted in quantitative analysis, creatinine at gradient concentration and deuterated creatinine at fixed concentration are added into an urine sample, direct analysis is performed, a standard addition curve is drawn, and the concentration of the urine creatinine is obtained. The urine creatinine can be directly measured under the condition that the sample is not pretreated, and the defects of a long analysis period and low efficiency in the prior art are overcome. The method has the characteristics of quickness, accuracy, capability of resisting interference from a substrate, and the like, and is particularly suitable for the clinical research of renal disease (such as the early diagnosis of acute renal failure) and high-flux sample analysis.

Description

technical field [0001] The invention relates to a method for detecting urine creatinine, in particular to an analysis method for rapid detection of urine creatinine by isotope dilution electrospray extraction ionization tandem mass spectrometry. Background technique [0002] Urinary creatinine is an important biomarker to characterize renal function, and it is of great significance to clinical research of renal diseases (such as early diagnosis of acute renal failure). The current methods for analyzing urine creatinine reported in the literature include: spectrophotometry, infrared spectroscopy, surface-enhanced Raman spectroscopy, gas chromatography-mass spectrometry (GC-MS), high-performance liquid chromatography (HPLC), liquid chromatography-mass spectrometry (LC) -MS) method, high performance thin layer chromatography (HPTLC) method, capillary electrophoresis method, enzymatic method, etc. Among the above methods, spectrophotometry is the most classic and commonly used ...

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Application Information

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IPC IPC(8): G01N27/64
Inventor 方小伟李雪于志强盛国英傅家谟陈焕文
Owner EAST CHINA UNIV OF TECH
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