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Mammal embryo and oocyte vitrification freezing carrier

A vitrification and oocyte technology, applied in the field of vitrification of mammalian embryos and oocytes, can solve the problems of unfavorable long-term storage of samples, increase the difficulty of operation, and significant siphon effect, so as to reduce freezing damage, It is convenient for long-term storage and has a remarkable cooling effect

Inactive Publication Date: 2012-09-19
HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The micro glass tube method is easy to break during the freezing process, which is easy to cause sample loss; the diameter and volume of GMP are small, which limits the number of samples frozen at one time, and the method has a significant siphon effect, which increases the difficulty of operation
The droplet method cannot be identified during storage, which is not conducive to the long-term storage of samples
Copper mesh method, Cryoloop and Cryotop all have sample loss during freezing and thawing

Method used

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  • Mammal embryo and oocyte vitrification freezing carrier
  • Mammal embryo and oocyte vitrification freezing carrier
  • Mammal embryo and oocyte vitrification freezing carrier

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Example 1: see figure 1 with figure 2 The vitrification carrier for mammalian embryos and oocytes includes a freezing thin tube 1 and a conical tip 7, which are matched and installed in a buckle.

[0022] figure 1 Wherein, the front end of the frozen thin tube 1 and the conical suction head 7 are provided with at least one longitudinal shearing opening 5, which is convenient for matching and fitting with the conical suction head and has tightness. figure 2 Wherein, the tip of the tapered tip 7 is provided with an incision 71, the other end is an installation opening 72, and the aperture at the incision 71 ranges from 100 μm to 200 μm. Install the conical tip 7 with an Eppendorf 20 μl pipette, put the carrier device into liquid nitrogen together, and insert the conical tip 7 holding the frozen sample into the cryotube 1 from the cut opening 5 with tweezers. After all the samples are frozen, put all the frozen straws 1 into the grapes in the liquid nitrogen tank, and put th...

Embodiment 2

[0024] Example 2: see image 3 , Figure 4 with Figure 5 The content is basically the same as the first embodiment, and the similarities are not repeated. The difference is that a hole 6 is provided on the side wall of the frozen thin tube contacted by the metal weight body 2. In addition, the front end of the metal weight body 2 is provided with a fork-shaped rib 21, the fork-shaped rib 21 passes through the partition and extends into the storage cavity 11, and is attached and fixed on the inner side wall of the freezing thin tube 1. The fork-shaped rib 21 not only has a strengthening and supporting effect on supporting the frozen thin tube, but also has the effect of accelerating cooling when connected with the metal weight body, and the cooling effect is remarkable.

Embodiment 3

[0025] Example three: see Image 6 The content is basically the same as the second embodiment, and the similarities will not be repeated. The difference is: the metal weight body 2 is exposed outside the frozen thin tube, and the metal weight body 2 is only partially fixed with the frozen thin tube 1. The front end of the weight body 2 is provided with a fork-shaped rib 21, the fork-shaped rib 21 passes through the partition 3 and then extends into the storage cavity 11, and is attached and fixed on the inner side wall of the freezing thin tube 1. The leaking metal weight body 2 is more conducive to heat conduction.

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Abstract

The invention discloses a mammal embryo and oocyte vitrification freezing carrier. The mammal embryo and oocyte vitrification freezing carrier comprises a freezing tubule and a tapered sucking head both of which are mounted in a matching and butting manner, wherein a metal weight body is arranged at the back end of the freezing tubule; and a sealing interlayer is arranged between the metal weightbody and an inner storage cavity of the freezing tubule. The mammal embryo and oocyte vitrification freezing carrier is low in cost as the manufacturing materials are the common materials, is easy tooperate and is capable of controlling amount of a freezing liquid precisely and freezing multiple oocytes and embryos for once. As the metal weight body is adopted, a sample cannot float on a liquid nitrogen surface and the sample cannot be lost; freezing damage of the sample is reduced due to the fast freezing speed and high freezing efficiency; and the carrier can be utilized repeatedly and cannot break to cause lost of the sample, so that cost is saved. Marks can be made on the tubule, so that convenience can be brought about for long-term preservation.

Description

technical field [0001] The invention relates to the technical field of cell vitrification, in particular to a mammalian embryo and oocyte vitrification carrier. Background technique [0002] Mammalian oocyte and embryo freezing is an important part of human and animal reproductive biology. In particular, the invention of vitrification can make freezing without the need of conventional freezing equipment, and put the frozen material into the freezing liquid and directly put it into liquid nitrogen, which greatly simplifies the procedure. Vitrification technology uses a tiny cryogenic carrier to turn a high-concentration cryoprotective solution into a glass-like solid with a strong viscosity during extremely fast cooling (>2000°C / min), avoiding intracellular ice crystals. The formation of cryoprotection can significantly improve the viability and development ability of cells and tissues after freezing. [0003] Commonly used vitrification methods are: nylon ring vitrifica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 施巧婷王二耀辛晓玲郎利敏陈俊峰张玉洋魏成斌水谷将也岛田浩明徐照学
Owner HENAN ACAD OF AGRI SCI
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