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Method of cuttage propagation of Maytenus guangxiensis seedlings by shoots of Maytenus guangxiensis

A technology of branches and seedlings, applied in the biological field, can solve the problems of difficult seed sources, limited sexual reproduction, low seed setting rate, etc., and achieve the effects of low production cost, maintaining excellent traits, and short growth cycle

Inactive Publication Date: 2012-09-19
GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Field surveys found that wild Guangxi Meideng wood not only has a low seed setting rate, but also has a high abortion rate, and the fruit falls off seriously early. The source of seeds is very difficult, and sexual reproduction is limited.
There is no report on the use of Guangxi Meideng wood cuttings to propagate seedlings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for using Guangxi Meideng wood branch cuttings to breed its seedlings, comprising the steps of:

[0019] (1) Before the test, 0.5% carbendazim solution was used to sterilize the cutting substrate. The cutting substrate was sand mixed with chaff ash, and the ratio of the material to the dosage was 1: 1; Lay the sterilized matrix in the middle, fill the prepared sand-mixed chaff ash into each slotting bed according to the test design, water it thoroughly, and rake it;

[0020] (2) Select plants that grow robustly and are free from diseases and insect pests to collect branches, and cut the branches after harvesting. The length of the cuttings is about 15 cm. Each cutting is guaranteed to have 2 to 3 complete and full buds. The upper incision is cut flat, and the lower incision is cut obliquely. The incision is smooth. Cuttings are terminal buds with two leaves;

[0021] (3) Soak the cut branches with 200 mg / L indole acetic acid (IAA) at the base for 8 hours;

...

Embodiment 2

[0026] A method for using Guangxi Meideng wood branch cuttings to breed its seedlings, comprising the steps of:

[0027] (1) The cutting substrate was sterilized with 0.5% carbendazim solution before the test, and the cutting substrate was yellow mud. Establish a sterilized seedbed on the selected breeding ground, lay a sterilized substrate in the seedbed, fill the prepared yellow mud into each slotting bed according to the experimental design, water it thoroughly, and rake it;

[0028] (2) Select healthy growing plants without pests and diseases to collect branches, and cut the branches immediately after harvesting; the length of the cuttings is about 15 cm, and each cutting is guaranteed to have 2 to 3 complete and full buds, and the upper incision is cut flat , The lower incision is obliquely cut, and the notch is smooth. The cuttings are the terminal buds with one leaf left.

[0029] (3) Soak the base of cut branches with 50 mg / L rooting powder (ABT) for 8 hours.

[003...

Embodiment 3

[0032] A method for using Guangxi Meideng wood branch cuttings to breed its seedlings, comprising the steps of:

[0033] (1) The cutting substrate was disinfected with 0.5% carbendazim solution before the test, and the cutting substrate was perlite. Establish a sterilized seedbed on the selected breeding ground, lay the sterilized substrate in the seedbed, fill the prepared perlite into each slotting bed according to the experimental design, water it thoroughly, and rake it;

[0034] (2) Select plants that grow robustly and are free from diseases and insect pests to collect branches, and cut the branches after harvesting. The length of the cuttings is about 15 cm. Each cutting is guaranteed to have 2 to 3 complete and full buds. The upper incision is cut flat, and the lower incision is cut obliquely. The incision is smooth. Cuttings are terminal buds with two leaves;

[0035] (3) Soak the base of cut branches with 50 mg / L indole butyric acid (IBA) for 4 hours.

[0036] All ...

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Abstract

The invention discloses a method of cuttage propagation of Maytenus guangxiensis seedlings by shoots of Maytenus guangxiensis. The method includes using shoots as explants, allowing the shoots to root and germinate to obtain complete plants, and transplanting the plants to build a reproduction system of Maytenus guangxiensis. The method has the outstanding advantages that growth period is short, production cost is low, quality is high, the method is simple to operate and easy to popularize and can be used to massively produce seedlings. In addition, the problems, such as recession and heteromorphosis, of the Maytenus guangxiensis seedlings bred by the method are avoided, superior character of the seedlings can be kept, safety in use of the seedlings is improved, and positive effect on regeneration and sustainable utilization of endangered medicinal plant resources in shortage is achieved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for propagating seedlings of Guangxi Meideng wood branches by cutting. Background technique [0002] Maytansia plants have long been used as medicinal resources for development and utilization. Their roots, stems, and leaves contain components such as maytansin, echinoic acid, succinic acid, and syringic acid. They have obvious inhibitory effects on human tumors and can be used to treat Breast cancer, gastrointestinal cancer, lymphosarcoma, bone marrow cancer, chronic myelogenous leukemia and other malignant tumors. Guangxi Meidengmu is a unique medicinal plant in Guangxi karst. Due to the deterioration of the living environment and the long-term indiscriminate logging by the people in the production areas, the number is getting smaller and smaller. Many areas are on the verge of extinction or have become extinct. Limestone Rare and Endangered Plant List" liste...

Claims

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Application Information

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IPC IPC(8): A01G9/10
Inventor 梁惠凌史艳财唐辉韦霄邹蓉孔德鑫王满莲周奇英陈宗游
Owner GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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