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Antibacterial peptide composition and preparation method thereof

An antimicrobial peptide and composition technology, applied in the field of bioengineering, can solve the problems of lack of hydrophilicity, unfavorable antimicrobial peptide structure, etc., and achieve the effects of good biocompatibility and hydrophilic performance, good therapeutic effect, and a wide range of sources.

Inactive Publication Date: 2012-09-12
YUNNAN MINZU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In terms of antimicrobial peptide sustained-release research, Chinese patent (CN102274485A) reported that polylactic acid-glycolic acid copolymer was used as a carrier to prepare antimicrobial peptide sustained-release carrier, and polylactic acid sustained-release microspheres were prepared. However, due to polylactic acid-glycolic acid copolymer It belongs to aliphatic polyester, lacks hydrophilicity, and is not conducive to stabilizing the structure of antimicrobial peptides. The purpose of the present invention is to use new materials to control the release of antimicrobial peptides according to the structure and performance of antimicrobial peptides, so as to solve the problem of antimicrobial peptide stability. Safety, safety, long-term release and improving the load capacity of materials for antimicrobial peptides

Method used

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  • Antibacterial peptide composition and preparation method thereof
  • Antibacterial peptide composition and preparation method thereof
  • Antibacterial peptide composition and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Weigh 1 part of antimicrobial peptide Pexiganan and dissolve it in 19 parts of ultrapure water to make an inner aqueous phase with an antimicrobial peptide content of 5%. At the same time, 10 parts of polylactic acid-polyethylene glycol copolymer were weighed and dissolved in 190 parts of dichloromethane to prepare a medium oil phase. In the case of mechanical stirring at 500rpm, the inner water phase was added to the middle oil phase and stirred for half an hour to form colostrum. Weigh 6 parts of polyvinyl alcohol and add to 214 parts of ultrapure water to make the outer water phase. Add the colostrum into the external water phase, and stir for 1 hour under the action of stirring at a rotation speed of 700 rpm to form a W / O / W double emulsion. Add 40 parts of isopropanol to the double emulsion, stir in the open for 3 hours, centrifuge at a speed of 10000 rpm, and freeze-dry the precipitate to obtain the antimicrobial peptide composition.

Embodiment 2

[0040] Weigh 1 part of antimicrobial peptide Hlf1-11 and dissolve it in 99 parts of ultrapure water to make an inner aqueous phase with an antimicrobial peptide content of 1%. At the same time, 10 parts of polylactic acid-polytrimethylene carbonate-polylysine copolymer were weighed and dissolved in 190 parts of dichloromethane to prepare a medium oil phase. In the case of mechanical stirring at 500 rpm, 10 parts of the inner water phase were added to 90 parts of the middle oil phase, and stirred for half an hour to form colostrum. Weigh 3 parts of polyvinyl alcohol and add to 97 parts of ultrapure water to make the outer water phase. Add 10 parts of colostrum into 80 parts of the external water phase, and stir for 1 hour under stirring at a rotation speed of 700 rpm to form W / O / W double emulsion. Add 20 parts of isopropanol to 100 parts of double emulsion, stir in the open for 3 hours, centrifuge at 12000 rpm, and freeze-dry the precipitate to obtain the antimicrobial pep...

Embodiment 3

[0042] Weigh 1 part of antimicrobial peptide Plectasin and dissolve it in 99 parts of ultrapure water to make an inner aqueous phase with an antimicrobial peptide content of 1%. At the same time, 1 part of polylactic acid-polyglycolic acid-polyethylene glycol copolymer was weighed and dissolved in 99 parts of dichloromethane to prepare a medium oil phase. In the case of mechanical stirring at 500 rpm, 10 parts of the inner water phase were added to 40 parts of the middle oil phase, and stirred for half an hour to form colostrum. Weigh 3 parts of polyvinyl alcohol and add to 97 parts of ultrapure water to make the outer water phase. Add 10 parts of colostrum into 80 parts of the external water phase, and stir for 1 hour under stirring at a rotation speed of 700 rpm to form W / O / W double emulsion. Add 30 parts of isopropanol to 100 parts of double emulsion, stir in the open for 3 hours, centrifuge at 9000 rpm, and freeze-dry the precipitate to obtain the antimicrobial peptide co...

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Abstract

The invention provides an antibacterial peptide composition and a preparation method thereof. Load is performed on antibacterial peptide by degradable high polymer material with both hydrophobicity and hydrophilicity to lead antibacterial peptide molecules to be loaded on the material to obtain antibacterial peptide composition. The specific load method comprises the following steps: the physical encapsulation method which is widely researched in literature is adopted to encapsulate antibacterial peptide in the center of the degradable high polymer material to prepare nanometer to nanometer microspheres; and when the material begins to degrade, antibacterial peptide is released gradually. The method adopts the degradable high polymer material with both hydrophobicity and hydrophilicity as the carrier. The degradable high polymer material has the similar structure as antibacterial peptide, better biocompatibility with the existing polylactic acid-glycollic acid copolymer and hydrophilicity, thus the composition is more beneficial for stabilizing the structure of antibacterial peptide. By adjusting the hydrophobicity, hydrophilicity and molecular weight of the material, adjustment of release time of antibacterial peptide can be realized, thus the composition is beneficial for satisfying various use needs and enlarging application range. The antibacterial peptide composition can be used as long-acting food preservative in the biomedicine field and the food freshness keeping field.

Description

technical field [0001] The invention relates to an antimicrobial peptide composition and a preparation method thereof, in particular to a long-acting slow-release dosage form of an antimicrobial peptide and a preparation method thereof, belonging to the field of bioengineering. Background technique [0002] In 1981, this substance with antibacterial activity was named cecropin, which was the first time that people actually discovered antibacterial peptides. Antimicrobial peptides are expected to become a new generation of antibiotics due to their high antibacterial activity and extremely low drug resistance. Antimicrobial peptides (AMPs) are an important part of biological intrinsic immunity, generally composed of 6-100 amino acid residues. Antimicrobial peptides generally have an amphiphilic structure, with a hydrophobic region binding to lipids and a positively charged hydrophilic region binding to water or negatively charged residues. These characteristics enable antimi...

Claims

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Application Information

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IPC IPC(8): A61K38/16A61K38/10A61K38/08A61K9/16A61K47/34A61P31/04A61P31/10A23L3/3526
Inventor 李宏利袁明龙李丽丽袁明伟于美玲
Owner YUNNAN MINZU UNIV
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