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Method for extracting and purifying saffron by-product petal and stamen total flavone

A purification method, saffron technology, applied in the direction of drug combination, pharmaceutical formula, plant raw materials, etc., can solve the problems of long extraction time, high energy consumption, complicated operation, etc., and achieve good reproducibility, low energy consumption, and extraction The effect of easy operation

Inactive Publication Date: 2012-09-12
SICHUAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The inventor of this patent application has used the Soxhlet extraction method to extract the total flavonoids of saffron petals and stamens, but the Soxhlet extraction method needs to be heated and refluxed, the operation is complicated, and the time required for extraction is too long (4~6 hours). The heating extraction will cause excessive energy consumption

Method used

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  • Method for extracting and purifying saffron by-product petal and stamen total flavone

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Extraction: use the dry powder of saffron petals and stamens as raw materials, and use ethanol aqueous solution with a volume fraction of 30% ethanol as the extraction solvent, according to the mass of raw materials (g): the volume of the extraction solution (mL) = 1:60 Ratio Put the raw materials and extraction solvent into the extraction container, extract for 90 minutes under the conditions of normal pressure, 25°C, and 150W ultrasonic power, filter, and concentrate the obtained filtrate under reduced pressure to obtain the extract; 3 Color development, the extraction rate of total flavonoids measured by ultraviolet spectrophotometry was 3.96%.

[0030] (2) Purification: Prepare the extract obtained in step (1) with distilled water to prepare a sample solution with a total flavonoid concentration of 0.6mg / mL, take 20BV of the sample solution and load it into AB-8 macropores at a flow rate of 0.5BV / h On the resin column, stand for adsorption for 2 hours, and the a...

Embodiment 2

[0033] (1) Extraction: use the dry powder of saffron petals and stamens as raw materials, and use ethanol aqueous solution with a volume fraction of 40% ethanol as the extraction solvent, according to the mass of raw materials (g): the volume of the extraction solution (mL) = 1:50 Ratio Put the raw materials and extraction solvent into the extraction container, extract at normal pressure, 25°C, and 180W ultrasonic power for 75 minutes, filter, and concentrate the obtained filtrate under reduced pressure to obtain the extract; 3 Color development, the extraction rate of total flavonoids measured by ultraviolet spectrophotometry was 4.66%.

[0034] (2) Purification: Prepare the extract obtained in step (1) with distilled water to prepare a sample solution with a total flavonoid concentration of 1.2mg / mL, take 10BV of the sample solution and load it on AB-8 macroporous resin at a flow rate of 1BV / h On the column, stand for adsorption for 3 hours, the adsorption rate of the macrop...

Embodiment 3

[0037] (1) Extraction: use the dry powder of saffron petals and stamens as raw materials, and use ethanol aqueous solution with a volume fraction of 50% ethanol as the extraction solvent. According to the mass of raw materials (g): volume of extraction solution (mL) = 1:40 Ratio Put the raw materials and extraction solvent into the extraction container, extract for 60 minutes under the conditions of normal pressure, 25°C, and 210W ultrasonic power, filter, and concentrate the obtained filtrate under reduced pressure to obtain the extract;3 Color development, the extraction rate of total flavonoids measured by ultraviolet spectrophotometry was 4.51%.

[0038] (2) Purification: Prepare the extract obtained in step (1) with distilled water to prepare a sample solution with a total flavonoid concentration of 1.5 mg / mL, take 8BV of the sample solution and load it on AB-8 macroporous resin at a flow rate of 2BV / h On the column, let it stand for adsorption for 4 hours, and the adsorpt...

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Abstract

The invention discloses a method for extracting and purifying saffron by-product petal and stamen total flavone. The method comprises the following processing steps of 1), extraction, namely adding dry powder of saffron petal and drying powder of stamen, which serves as raw materials and an ethanol aqueous solution serving as an extraction solvent into an extraction container in a ratio of 1g: (10-60)mL, performing ultrasound extraction for 15 to 90 min at the temperature of 25 DEG C under normal pressure, filtering, and concentrating an obtained filtrate under reduced pressure to obtain extractum; and 2), purification, namely preparing a loading solution by adding water into the extractum obtained in the step (1), loading onto a macroporous resin column, standing for adsorption for at least two hours, eluting by using the water after adsorption is finished, and discarding eluent, eluting by using the ethanol aqueous solution again, collecting the eluent, concentrating the eluent under reduced pressure, performing vacuum drying till constant weight to obtain the purified saffron petal and stamen total flavone.

Description

technical field [0001] The invention belongs to the field of extracting and purifying active ingredients of traditional Chinese medicines, and in particular relates to a method for extracting and purifying total flavonoids of saffron by-product petals and stamens. Background technique [0002] Saffron petals and stamens are dried petals and stamens of the bulbous herbaceous plant Saffron (Crocus sativus) of the Iridaceae Saffron genus. Saffron is native to southern Europe and Iran, and is cultivated in Shanghai, Zhejiang, Jiangsu, Shandong, Beijing and other places in my country. The stigma of saffron is its main medicinal part, which has the effects of promoting blood circulation and removing blood stasis, cooling blood and detoxification, relieving stagnation and calming nerves, can inhibit hepatitis virus, improve immunity, and has anticancer activity and low toxicity pharmacological effects. gold". In addition, saffron is also used as a precious flavoring agent, natura...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/88A61P9/10A61P9/12A61P9/00A61P39/06A61P43/00A61K133/00
Inventor 白洁王晓萌陈放普廷科
Owner SICHUAN UNIV
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