Formula feed for chickens and preparation method thereof
A technology for compound feed and chicks, which is applied in the field of compound feed for chicks and its preparation, can solve the problems of late start of research, lack of in-depth and systematic research materials, blank product development, etc., achieve strong resistance to stress, reduce diarrhea rate and mortality rate , the effect of improving animal immunity
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Embodiment 1
[0023] Example 1 Screening of Enterococcus faecalis CGMCC No.5092
[0024] 1. Primary screening
[0025] Take 5g samples of pig manure, cow manure and chicken manure, etc., soak them in 20ml of sterile 0.85% normal saline, oscillate to break up the samples, make the samples fully contact with the normal saline, let them stand in a constant temperature water bath at 70°C for 3 minutes, take The supernatant was centrifuged at 5000 rpm for 10 minutes, and the supernatant was discarded. Add the centrifuged bacterial cell pellet to 10ml of artificial gastric juice, let it stand at 37°C for 2 hours, then centrifuge to collect the bacterial cell, add the bacterial cell pellet to 10ml of artificial bile salt, let it stand at 37°C for 4 hours, collect the bacterial cell, dilute and apply Spread on LB medium. After culturing in a 37°C incubator for 12-24 hours, the colonies were purified and isolated. The obtained bacteria were preserved and further re-screened.
[0026] The prepara...
Embodiment 2
[0033] Example 2 Verification of Stress Resistance of Enterococcus faecalis
[0034] (1) High temperature resistance
[0035] Collect Enterococcus faecalis cultured for 12 hours by centrifugation, put them into normal saline for resuspension, take samples and spread them, and count the number of viable bacteria; then heat the normal saline in a water bath at 70°C for ten minutes, cool it down rapidly, and then Plate counting was carried out, and the number of residual viable bacteria was 85.3%.
[0036] (2) Tolerance of artificial gastric juice
[0037] Take 0.5ml of Enterococcus faecalis cultured for 12h, dissolve it in 4.5ml of normal saline, and use the plate count method to count the number of viable bacteria. Then take the quantitative above-mentioned bacteria solution and add it to 4.5ml of artificial bile salt medium, let it stand at 37°C for 2h, count the number of remaining viable bacteria by plate counting method, and the number of remaining viable bacteria is 92.2...
Embodiment 3
[0041] Example 3 The probiotic function of Enterococcus faecalis (CGMCC No.5092)
[0042] (1) Verification of antibacterial function
[0043] Pathogenic Escherichia coli K88, K99 and Staphylococcus aureus cultured overnight were counted by plate counting method. Add 5ml of Enterococcus faecalis bacteria solution to 3 tubes of 5ml Escherichia coli K88, K99 and Staphylococcus aureus respectively. Cultivate at 37°C for 4 hours, and calculate the number of viable bacteria of Escherichia coli K88, K99 and Staphylococcus aureus remaining by plate colony counting method. The remaining pathogenic Escherichia coli K88, K99 and Staphylococcus aureus were 24.7%, 30.2% and 31.8%.
[0044] (2) Verification of mold inhibition in corn flour
[0045] Take 20ml of Enterococcus faecalis cultured for 12 hours and mix it with 30g of corn flour. For the blank experiment, mix 20ml of sterile water with 30g of corn flour. The corn flour and the growth of mold were observed. Experiments show tha...
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