Formula feed for chickens and preparation method thereof

A technology for compound feed and chicks, which is applied in the field of compound feed for chicks and its preparation, can solve the problems of late start of research, lack of in-depth and systematic research materials, blank product development, etc., achieve strong resistance to stress, reduce diarrhea rate and mortality rate , the effect of improving animal immunity

Active Publication Date: 2014-09-17
BEIJING DABEINONG TECH GRP CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on compound feed for 0-14-day-old egg chicks started relatively late, and there is a lack of in-depth and systematic research data at home and abroad, and product development is almost blank.

Method used

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  • Formula feed for chickens and preparation method thereof
  • Formula feed for chickens and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Screening of Enterococcus faecalis CGMCC No.5092

[0024] 1. Primary screening

[0025] Take 5g samples of pig manure, cow manure and chicken manure, etc., soak them in 20ml of sterile 0.85% normal saline, oscillate to break up the samples, make the samples fully contact with the normal saline, let them stand in a constant temperature water bath at 70°C for 3 minutes, take The supernatant was centrifuged at 5000 rpm for 10 minutes, and the supernatant was discarded. Add the centrifuged bacterial cell pellet to 10ml of artificial gastric juice, let it stand at 37°C for 2 hours, then centrifuge to collect the bacterial cell, add the bacterial cell pellet to 10ml of artificial bile salt, let it stand at 37°C for 4 hours, collect the bacterial cell, dilute and apply Spread on LB medium. After culturing in a 37°C incubator for 12-24 hours, the colonies were purified and isolated. The obtained bacteria were preserved and further re-screened.

[0026] The prepara...

Embodiment 2

[0033] Example 2 Verification of Stress Resistance of Enterococcus faecalis

[0034] (1) High temperature resistance

[0035] Collect Enterococcus faecalis cultured for 12 hours by centrifugation, put them into normal saline for resuspension, take samples and spread them, and count the number of viable bacteria; then heat the normal saline in a water bath at 70°C for ten minutes, cool it down rapidly, and then Plate counting was carried out, and the number of residual viable bacteria was 85.3%.

[0036] (2) Tolerance of artificial gastric juice

[0037] Take 0.5ml of Enterococcus faecalis cultured for 12h, dissolve it in 4.5ml of normal saline, and use the plate count method to count the number of viable bacteria. Then take the quantitative above-mentioned bacteria solution and add it to 4.5ml of artificial bile salt medium, let it stand at 37°C for 2h, count the number of remaining viable bacteria by plate counting method, and the number of remaining viable bacteria is 92.2...

Embodiment 3

[0041] Example 3 The probiotic function of Enterococcus faecalis (CGMCC No.5092)

[0042] (1) Verification of antibacterial function

[0043] Pathogenic Escherichia coli K88, K99 and Staphylococcus aureus cultured overnight were counted by plate counting method. Add 5ml of Enterococcus faecalis bacteria solution to 3 tubes of 5ml Escherichia coli K88, K99 and Staphylococcus aureus respectively. Cultivate at 37°C for 4 hours, and calculate the number of viable bacteria of Escherichia coli K88, K99 and Staphylococcus aureus remaining by plate colony counting method. The remaining pathogenic Escherichia coli K88, K99 and Staphylococcus aureus were 24.7%, 30.2% and 31.8%.

[0044] (2) Verification of mold inhibition in corn flour

[0045] Take 20ml of Enterococcus faecalis cultured for 12 hours and mix it with 30g of corn flour. For the blank experiment, mix 20ml of sterile water with 30g of corn flour. The corn flour and the growth of mold were observed. Experiments show tha...

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Abstract

The present invention discloses a formula feed for chickens, comprising components of the following weight ratios: 500 to 600g / kg of puffed corn, 140 to 180g / kg of puffed and peeled soybean meal, 60 to 100g / kg of puffed soybean, 40 to 80g / kg of fermented soybean meal, 30 to 50g / kg of glucose, 30 to 50g / kg of fish meal, 20 to 40g / kg of whey powder, 10 to15g / kg of stone powder, 6 to 12g / kg of calcium hydrogen phosphate, 4.5 to 5.5g / kg of chicken compound premix, 2.5 to 3.5g / kg of compound acidifiers, 2 to 2.5g / kg of methionine, 1.5 to 2.5g / kg of salt, 0.25 to 0.75g / kg of choline chloride, 0.4 to 0.6g / kg of enterococcus faecalis, 0.15 to 0.25g / kg[of] poultry compound enzyme, 0.05 to 0.15g / kg of vitamin C, and 0.066 to 0.198g / kg of ethoxyquin. The formula feed for chickens, balanced in nutrition and high in digestibility rate, can effectively reduce a diarrhoea rate and a death rate of chickens, can facilitate the increase of weight and the growth of shin bones, and can guarantee healthy and rapid growth of chickens.

Description

technical field [0001] The invention belongs to the field of animal feed, and in particular relates to a chicken compound feed and a preparation method thereof. Background technique [0002] The breeding of laying hens is developing in the direction of miniaturization and high nutritional requirements. Layer chicks aged 0-14 days are affected by stress such as beak trimming, immunity, and pathogens, and there is no special feed for the brooding period, especially the early stage of brooding (0-14 days old) in current laying hen farming. Therefore, the weight and tibia length of laying hens are often not up to standard, which affects the production performance of laying hens throughout the cycle. Due to the poor thermoregulation ability and immature immune organ development in the chick stage, it is easy to be infected with bacteria and diseases, and has high nutritional requirements. The digestive system is not well developed, and the digestibility of raw materials is very...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/18A23K1/16
Inventor 胡忠宏任建波俞云涛应广飞刘明忠张立彬黄翠英高雅琪
Owner BEIJING DABEINONG TECH GRP CO LTD
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