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Composite mutation method for breeding two-line glabrous sterile line of rice

A compound mutagenesis and rice technology, which is applied in the direction of botany equipment and methods, application, plant gene improvement, etc., can solve the problems of heavy workload, low benefit, time-consuming and labor-intensive breeding, and achieve high breeding efficiency, short time effect

Inactive Publication Date: 2012-09-12
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the method of introducing naked genes into maintainer lines, sterile lines, and restorer lines through traditional hybridization and backcrossing methods results in a large selection workload due to gene recombination and separation, and a long breeding period. Breeding lines or hybrid rice combinations generally take 5-7 years, which is time-consuming and labor-intensive, and the benefits are low
Genetic studies have shown that rice nakedness traits can be obtained by mutagenesis, but the traditional method of mutagenizing rice dry seeds has low mutation frequency and poor mutagenesis effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1) Take 650 grams of seeds of the two-line CMS "Peiai 64S", soak the seeds for 24 hours, drain the water, put them in a germination box at 30 degrees for 18-36 hours, until the seed buds are white. Soak the seeds in a mixture of chemical mutagen manganese sulfate and diethyl sulfate (DES) (the mass concentration of manganese sulfate and diethyl sulfate is both 0.2%) for 4 hours, rinse the seeds with water twice after treatment, and drain the water Drying, using microwave radiation with a radiation frequency of 3000MHz and a power of 700W for 8 seconds, followed by radiation for 8 seconds after an interval of 1 minute, and 4 consecutive treatments;

[0017] 2) Sow the "Pei'ai 64S" seeds treated with mutagenesis, and plant them in dense clusters, M 1 Plant seeds (M 2 ) are harvested and dried at maturity;

[0018] 3) M 2 Seeds are thinly sown and wait for M 2 When the five-leaf stage is reached, the naked mutants are screened and identified, and the rice seedlings det...

Embodiment 2

[0022] 1) Take 600 grams of seeds of the two-line CMS "Guangzhan 63S", soak the seeds for 24 hours, drain the water, put them in a germination box at 30 degrees for 18-36 hours, until the seed buds are white. Soak the seeds in a mixture of chemical mutagen manganese sulfate and diethyl sulfate (DES) (the mass concentration of manganese sulfate and diethyl sulfate is both 0.2%) for 4 hours, rinse the seeds with water twice after treatment, and drain the water Drying, with a radiation frequency of 3000MHz and a power of 750W microwave radiation treatment for 10 seconds, followed by radiation treatment for 10 seconds after an interval of 1 minute, and 4 consecutive treatments;

[0023] 2) Sowing the "Guangzhan 63S" seeds treated with mutagenesis, densely planted in clusters, M 1 Plant seeds (M 2 ) are harvested and dried at maturity;

[0024] 3) M 2 Seeds are thinly sown and wait for M 2 When the five-leaf stage is reached, the naked mutants are screened and identified, and t...

Embodiment 3

[0028] 1) Take 600 grams of seeds of the two-line sterile line "P88S", soak the seeds for 24 hours, drain the water, put them in a germination box at 30 degrees for 18-36 hours, until the seed buds are white. Soak the seeds in a mixture of chemical mutagen manganese sulfate and diethyl sulfate (DES) (the mass concentration of manganese sulfate and diethyl sulfate is both 0.2%) for 4 hours, rinse the seeds with water twice after treatment, and drain the water To dry, use microwave radiation with a radiation frequency of 3000MHz and a power of 800W for 9 seconds, then 9 seconds after an interval of 1 minute, and 4 consecutive treatments;

[0029] 2) Sowing the "P88S" seeds treated with mutagenesis, densely planted in clusters, M 1 Plant seeds (M 2 ) are harvested and dried at maturity;

[0030] 3) M 2 Seeds are thinly sown and wait for M 2 When the five-leaf stage is reached, the naked mutants are screened and identified, and the rice seedlings determined to be naked mutants...

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Abstract

The invention discloses a composite mutation method for breeding a two-line glabrous sterile line of rice, and belongs to the technical field of rice heterosis utilization. The glabrous character of rice is controlled by a pair of recessive genes, and a glabrous mutant can be obtained through mutagenesis. Chemical mutagens, namely manganese sulfate and diethyl sulfate, are mixed for treating a two-line sterile line (S line) sprout seed, microwaves are adopted for radiation, a rice mutant with glabrous character mutation is obtained, and the two-line glabrous sterile line (glabrous S) with the glabrous character is bred through character selection. The glabrous S can be combined with a glabrous restorer to form two-line glabrous hybrid rice. The rice sprout seed is treated by the composite mutation method, the mutation frequency of the glabrous character is 3 to 4 times higher than that of a method for treating a rice dry seed by singly using radiation or chemical mutagenesis, and the mutation frequency is 0.03 to 0.04 percent. The method for breeding the two-line glabrous sterile line is short in time and high in efficiency, and the whole breeding process only requires 3-4 planting generations, namely 1-2 years.

Description

technical field [0001] The invention specifically relates to a method for breeding a two-line naked sterile line of rice by using a compound mutagenesis method, and belongs to the technical field of rice heterosis utilization. Background technique [0002] As the name suggests, "Naked Rice" is rice that is hairless all over the body, so it is not easy for pests to attach and invade, and it has strong resistance to pests and diseases. It can reduce skin itching and damage to the skin during manual harvesting and threshing. Processing There is no dust at all times, which can reduce environmental pollution, so it is also called "environmentally friendly rice". And naked rice is also of great value in terms of increasing the storage capacity of rice and being easy to mechanized harvesting. At present, naked rice varieties have been initially paid attention to and popularized in my country. The Agricultural Science Institute of Hengyang City, Hunan Province used the resources of...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/06
Inventor 章清杞李美德陈幼玉
Owner FUJIAN AGRI & FORESTRY UNIV
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