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Amaroucium constellatum lipophilic extract

A sea sphingolipid and extract technology, which is applied in the direction of food science, drug combination, metabolic diseases, etc., achieves the effect of simple preparation process, good blood lipid elevation, and prevention and treatment of blood lipid elevation

Inactive Publication Date: 2013-10-30
EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no literature report on the application of the lipid-soluble extract of Ascidian americana in medicine and functional food

Method used

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  • Amaroucium constellatum lipophilic extract
  • Amaroucium constellatum lipophilic extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Constellation American sea squirt -18 ℃ frozen individuals take 2.0kg each time, directly add 2L ethyl acetate, grind and homogenate for 6 minutes, centrifuge at 4 ℃ low temperature, the speed is 6500 rpm; collect the upper layer of ethyl acetate clear liquid; the lower layer of water and minced meat Add 2.0L ethyl acetate to the mixture and quickly repeat the process of homogenization and centrifugation twice; combine the upper ethyl acetate clear liquid for three times, recover the solvent and dry to obtain a yellowish-brown viscous oil, namely the fat-soluble extract of Ascidian americana . The extraction is repeated several times, the product is between 24.8-31.2 grams, and the extraction rate is 1.24-1.56%.

[0014] The relative content of polyunsaturated fatty acid compounds in the fat-soluble extract of Ascidian americana is analyzed by gas chromatography-mass spectrometry (GC-MS). Fatty acid methyl esters were prepared according to the animal and vegetable oil ...

Embodiment 2

[0018] The fat-soluble extract of Ascidian americana extracted according to Example 1 was suspended in petroleum ether, and then washed three times with 60% aqueous methanol to remove some large polar components. The petroleum ether part was taken and dried by recovering the solvent to obtain a light yellow-brown viscous oil with an extraction rate of 0.90-1.24%. According to gas chromatography-mass spectrometry analysis, the relative content of EPA+DHA in the fat-soluble oil extracted by this method is 35.2-40.7%.

Embodiment 3

[0020] According to Example 1, after thawing, slicing, and crushing individual Ascidians americana, the added ethyl acetate can be replaced by 80% aqueous methanol, soaked and extracted three times at room temperature, each time for 3 days, the extracts are combined, and the solvent is recovered and then dried to obtain a dark brown viscous Thick paste, namely Constellation American Sea Squirrel Total Extract. Dissolve the total extract of Ascidian americana in 60% aqueous methanol, then extract three times with petroleum ether, combine the petroleum ether parts, and dry them after recovering the solvent to obtain a yellow-brown viscous oil, which is the fat-soluble part of Ascidian americana. The extraction is repeated many times, the product is between 56-81 grams, and the extraction rate is 1.12-1.62%.

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Abstract

The invention discloses an amaroucium constellatum lipophilic extract, which relates to preparation and the application of the amaroucium constellatum extract in the field of the biomedicine. In the amaroucium constellatum lipophilic extract disclosed by the invention, ethyl acetate is directly added into an amaroucium constellatum individual to form mixture, the mixture is smashed, homogenized and centrifugated, upper-layer ethyl acetate clear liquid is taken, and the ethyl acetate clear liquid is dried after solvent is recovered. The amaroucium constellatum lipophilic extract has the characteristics that an -18 DEG C freezing individual of the amaroucium constellatum is weighed; 1L of solvent is directly added into each kg of constellatum, i.e. the ethyl acetate of one-time volume is added to form low-temperature mixture; the low-temperature mixture is smashed and homogenized for 6min; the low-temperature mixture is centrifugated at the low temperature of 4DEG C for 6 min at the revolving speed of 6500turns / min; after centrifugation, the upper-layer ethyl acetate clear liquid is poured and collected; the ethyl acetate of one-time volume is added into lower-layer mixture of water and meat paste to repeat the homogenizing and centrifugating processes for two times; the upper-layer ethyl acetate clear liquid of each time is collected; the upper-layer ethyl acetate clear liquid obtained in three times is combined; and the solvent ethyl acetate is recovered by pressure reduction and concentration and is dried to obtain thick grease, i.e. the amaroucium constellatum lipophilic extract. The amaroucium constellatum lipophilic extract is used for medicines or functional food with function of preventing blood fat from rising.

Description

technical field [0001] The invention relates to the preparation and application of the extract of Ascidian americana in the field of biomedicine. Background technique [0002] Constellation American sea squirt, also known as constellation fold stomach sea squirt, constellation column fine sea squirt, Latin name Amaroucium constellatum Verrill, foreign also named Aplidium constellatum Verrill; for Ascidiacea (Ascidiacea), simple branch suborder (Aplousobranchia), three sections of Ascidae ( Polyclinidae) sea squirts are common marine periphytons in the southeast coast of my country; experiments have shown that the extracts of American sea squirts, especially the ethyl acetate extraction parts, showed strong cytotoxicity to Artemia salina nauplii, suggesting that the fat-soluble parts May have certain anti-tumor activity; the results can be found in the following literature: Fang Yuchun, Song Jin, Zhu Tianjiao, etc. Screening of anti-tumor activity of coastal marine organisms in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/56A61P3/06A23L1/29A23L33/00A61K35/655
Inventor 樊成奇陆亚男翟尚华杨桥邵盛男
Owner EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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