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Method for preparing natural bamboo fibers in pectin removal manner by aid of compound microorganism bactericide

A technology of microbial bacterial agent and composite bacterial agent, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of destroying bamboo fiber bundles, environmental pollution, long cycle, etc., and achieve stable and optimized fiber quality Process conditions, the effect of high fiber strength

Inactive Publication Date: 2012-08-15
MIANYANG TEACHERS COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the initial stage of bamboo fiber development and research, bamboo fibers were basically produced by chemical methods, such as in Chinese patent applications No. If the bamboo fiber bundles are lost, the product will easily lose its ecological properties, and the sewage after chemical treatment is difficult to treat, causing environmental pollution
However, using biophysical technology (No. 200710050068.5) and biochemical softener (No. 200710050069.5) to soften and extract bamboo fiber is still in the laboratory stage and has not yet achieved the stage of industrialization.
The patent applications related to the preparation of bamboo fiber by microbial degumming mainly include No.200610039608.0, No.200810121092.3 and No.201010300575.7, all of which use a single biological enzyme or a compound biological enzyme to treat bamboo, but the cycle is long, generally it takes 3~ 7 days, and have higher requirements on equipment and process conditions

Method used

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  • Method for preparing natural bamboo fibers in pectin removal manner by aid of compound microorganism bactericide
  • Method for preparing natural bamboo fibers in pectin removal manner by aid of compound microorganism bactericide
  • Method for preparing natural bamboo fibers in pectin removal manner by aid of compound microorganism bactericide

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Effect test

Embodiment 1

[0039] The preparation of embodiment 1 composite bacterial agent

[0040] Special microorganisms with high efficiency in degrading pectin, lignin, hemicellulose and pentosan in rough bamboo fibrils are prepared into composite bacterial agents according to the following volume percentages:

[0041] Compound Bacteria Formula I:

[0042]

[0043] Compound Bacteria Formula II:

[0044]

[0045] Compound Bacteria Formula III:

[0046]

[0047]

[0048] Compound Bacteria Formula IV:

[0049]

[0050] Compound Bacteria Formula V:

[0051]

[0052] Among the above-mentioned composite bacterial agent formulas I~V, the microbial bacterial agent of depectin contains 4×10 7 Actinomycetes spores / ml; the microbial agent for degrading lignin contains 5×10 8 fungal spores / ml; the microbial agent for degrading lignin contains 2×10 bacteria 7 CFU / ml; the microbial agent for degrading hemicellulose and pentosan contains 2×10 8 fungal spores / ml.

[0053] Wherein, the act...

Embodiment 2

[0054] The degradation of embodiment 2 crude bamboo fiber pectin

[0055] Micromonospora bronze (Micromonosporachalcea) in the genus Micromonospora (Micromonospora), which is a special strain for producing pectinase, was activated, then transferred to the fermentation medium, and cultivated at 25 ° C for 20 h, as the second One-time degumming bacterial classification, degumming fermented broth bacterial classification can be recycled repeatedly, adds in the above-mentioned fermented liquor after thick bamboo fiber is weighed (mass volume ratio g of thick bamboo fibril and fermented liquor: ml is 1: 25, normal pressure After degumming the pectinase-producing bacteria for 48 hours, rinse and dry with water to observe the degumming effect, compare the residual glue before and after degumming, the residual glue rate is 11.4%, and the color of the bamboo fiber after degumming is lighter than that before degumming The formula of pectin degradation fermentation medium is as follows: ...

Embodiment 3

[0057] Degradation of embodiment 3 thick bamboo raw fiber lignin

[0058] Degradation of lignin: the special bacterial species (fungus being the basidiomycetes (Baidimycetes) in the brown rot fungus (Monilia laxa) ) of the lignin degrading enzyme produced after optimization, the bacterium being the aeruginosa (Pseudomonas) in the genus (Pseudomonas) Pseudomonas (P.Aeruginosa)) were respectively activated by liquid medium, in which the fungi were cultured at 32°C for 2 days, and the bacteria were cultured at 37°C for 24 hours, so that the concentrations of the fungi and bacteria reached 1×10 8 Fungal spores / ml, 1×10 6 ~1×10 7 CFU / ml. Then add fungi and bacteria to the fungi and bacteria fermentation medium according to the inoculum amount of 5-15%, adjust the pH value to 7.0, and finally weigh the bamboo fiber after depectin and add it to the fungi and bacteria to produce lignin degradation The enzyme was degummed in the fermentation medium for 48 hours (the mass volume rati...

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Abstract

The invention provides a method for preparing natural bamboo fibers in a pectin removal manner by the aid of compound microorganism bactericide. The method includes steps of removing pectin of coarse natural bamboo fibers via microorganisms for removing pectin, microorganisms for decomposing lignin and microorganisms for decomposing hemicellulose and pentosan successively; then adding the compound microorganism bactericide into the natural bamboo fibers to remove pectin; and drying after refining via alkali and auxiliaries to obtain the natural bamboo fibers. The thickness and the diameter of the natural bamboo fibers are controlled by means of changing constitution, proportion, decomposition time and the like of the compound microorganism bactericide. The natural bamboo fibers produced by the method are high in strength and fine in toughness and are environment-friendly, a total pectin removal process of the microorganism method is water-saving, energy-saving and free of wastewater, waste slag and waste gas, microorganism treatment liquid and cleaning liquid can be recycled completely, and the problem of environmental pollution caused by chemical treatment is resolved effectively. Accordingly, the method has the advantages of easiness in obtaining of raw materials, simplicity in operation, high extraction efficiency, stable quality of the fibers, and the like.

Description

technical field [0001] The invention relates to a method for preparing natural green and environment-friendly bamboo fiber, in particular to a method for preparing bamboo fiber with different fiber lengths and diameters by using thick bamboo fiber as a raw material and degumming with microbial compound bacteria agents. Background technique [0002] Bamboo industry is one of the four sunrise industries of my country's forestry industry, and has an overall competitive advantage in the international market. Bamboo is a perennial plant with short production period, high yield, strong regeneration and easy cultivation. It has the characteristics of maintaining water and soil, purifying and storing water sources, absorbing carbon dioxide, and reducing the greenhouse effect. Therefore, the bamboo industry has become a new economic growth point of my country's forestry development in recent years, and the development and use of bamboo fiber is more favored by scientific and technolo...

Claims

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Application Information

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IPC IPC(8): D01C1/04C12S11/00C12N1/14C12N1/20C12R1/365C12R1/29C12R1/645C12R1/38C12R1/07
Inventor 阮期平
Owner MIANYANG TEACHERS COLLEGE
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