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Production method for microbial oil

A microbial oil and production method technology, which is applied in the direction of microorganism-based methods, microorganisms, biochemical equipment and methods, etc., can solve the problems that cannot meet the needs of industrialized production of microbial oil and low oil yield, and achieve low cost and easy operation. The effect of simplicity and simple fermentation process

Active Publication Date: 2012-08-15
GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for most oil microorganisms, the oil yield is very low, which cannot meet the needs of industrial production of microbial oil

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Crush the corncob to 20 mesh, add dilute sulfuric acid according to the solid-liquid ratio of corncob and dilute sulfuric acid (concentration: 2wt%) is 1g: 10mL, hydrolyze at 125°C for 1.5h, filter, and use calcium hydroxide to hydrolyze The pH of the solution was adjusted to 7.0, and filtered to obtain a corncob dilute acid hydrolyzate with a total sugar concentration of 40 g / L.

[0025] Mix corncob dilute acid hydrolyzate and fermentation medium (xylose 60g / L, peptone 1.8g / L, yeast powder 0.5g / L, balance water) at a volume ratio of 10:90 to prepare acclimation medium. Trichosporn coremiiforme CCIC 1256 was used as the starting strain, inoculated into the acclimatization medium, adjusted to pH 5.0 with acetic acid, and cultured at 30°C. Gradually increase the proportion of corncob dilute acid hydrolyzate in the acclimation medium by 5% per day until the proportion of corncob dilute acid hydrolyzate in the acclimatization medium is 100%, and continue to cultivate for 10...

Embodiment 2

[0029] Preparation of enzyme hydrolyzate of rice straw:

[0030] 1) Acid pretreatment: crush rice straw to 20 mesh, add dilute sulfuric acid according to the solid-to-liquid ratio of rice straw to dilute sulfuric acid (concentration: 2wt%) is 1g:8mL, and hydrolyze at 130°C for 3h.

[0031] 2) Alkali pretreatment: after acid pretreatment, the hydrolyzate was filtered to collect solids, washed with water, and then added NaOH solution (concentration: 1.5wt%) according to the ratio of solid-liquid ratio of 1g: 6ml, at 100°C and 160r / min Processing 2h.

[0032]3) Enzymolysis: filter the rice straw hydrolyzate after acid and alkali pretreatment to collect solids, wash with water, and dry. ) / gram of cellulose by adding two kinds of enzymes, enzymolysis at 50°C and 180r / min for 3.5 days to obtain rice straw enzymatic hydrolyzate, the total sugar concentration is 100g / L, and its pH value is 5.0.

[0033] The rice straw enzymatic hydrolyzate and fermentation medium (60 g / L xylose, 1.8...

Embodiment 3

[0037] Carry out enzymatic hydrolysis to rice straw according to example 2, obtain rice straw enzymatic hydrolyzate, total sugar concentration is 100g / L, its pH value is 5.0, add water and dilute into total sugar concentration and be 50g / L rice straw enzymatic hydrolyzate.

[0038] The rice straw enzymatic hydrolyzate and fermentation medium (60g / L xylose, 1.8g / L peptone, 0.5g / L yeast powder, and water as the balance) were mixed according to the ratio of 15:85 to prepare acclimation medium. Trichosporon dermatis CCIC 32903 was used as the starting strain, inoculated into the acclimation medium, adjusted to pH 6.5 with NaOH solution (concentration: 1.5 wt%), and cultured at 25°C. Gradually increase the ratio of the rice straw enzyme hydrolyzate in the domestication medium by 5% per day until the ratio of the rice straw enzyme hydrolyzate in the domestication medium is 100%, and continue to cultivate for 12 days to obtain the domesticated liposome. The oil yield coefficient in t...

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Abstract

The invention discloses a production method for microbial oil. In the invention, oil yeast is obtained by domesticating the yeast, the domestication method is simple to operate, the oil yield coefficient of the domesticated oil yeast in lignocellulose hydrolysate is 13-20% which is higher than that of before the domestication processing by 20%-30%, the domesticated oil yeast is inoculated into the lignocellulose hydrolysate for fermentation, then thallus in the fermentation liquor is collected and broken, extracted by an organic solvent, and then the organic solvent is removed so as to obtain the microbial oil. In the method, the lignocellulose hydrolysate is not required for detoxification and the other treatment, only simple pretreatment is conducted on the lignocellulose acid hydrolysate, no nitrogen source and trace elements are added in the lignocellulose hydrolysate, and after yeast strains are inoculated, the microbial oil can be obtained by efficiently using different saccharide materials and fermenting lignocellulose hydrolysate; and the fermentation process is simple, and the cost is low.

Description

technical field [0001] The invention relates to a method for producing microbial oil, in particular to a method for producing microbial oil by fermenting lignocellulose hydrolyzate with yeast Trichosporon dermatis and Trichosporn coremiiforme. Background technique: [0002] Microbial oil, also known as single cell oil (SCO), refers to the glyceride synthesized by microorganisms under certain conditions and stored in the bacteria. Its fatty acid composition is similar to that of general vegetable oil, mainly C 16 、C 18 Department of fatty acids, such as palmitic acid, stearic acid, oleic acid and linolenic acid. Certain microorganisms such as bacteria, yeast, mold, and microalgae can use carbohydrates, hydrocarbons, and ordinary oils as carbon sources to accumulate a large amount of oil. If the amount of oil accumulation exceeds 20% of the dry weight of the cell, it is called an oleaginous microorganism ( Oleaginous microorganisms). [0003] In the past two decades, functi...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12R1/645
Inventor 陈新德黄超熊莲陈雪芳张海荣
Owner GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI
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