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Method for foot-and-mouth disease vaccine concentration and purification

A technology of foot-and-mouth disease vaccine and purification method, which is applied in chemical instruments and methods, peptide preparation methods, organic chemistry, etc., can solve the problems of vaccine safety and unstable immune effect, and achieve less adverse reactions and better immune effect

Inactive Publication Date: 2012-08-15
JINYUBAOLING BIO PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a new method for the concentration and purification of high-efficiency foot-and-mouth disease virus antigens to solve the problem of unstable safety and immune effect of foot-and-mouth disease inactivated vaccines

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: foot-and-mouth disease type O inactivated vaccine manufacture, inoculate BHK21 cell suspension culture with foot-and-mouth disease type O virus OZK / 93 strain and OR / 80 strain respectively, harvest cell culture, after the inactivation of divinylimine (BEI) respectively , and then processed by the following methods, adding mineral oil adjuvant to mix and emulsify to make a vaccine, which is used to prevent swine type O foot-and-mouth disease.

[0020] (1) Use a continuous flow centrifuge to remove large particulate matter in the antigen

[0021] ① Use a continuous flow centrifuge, set the speed at 3000 rpm.

[0022] ② Collect the light liquid discharged from the centrifuge and discard the sediment (heavy liquid).

[0023] (2), process the antigen solution after the step (1) with a hollow fiber ultrafiltration system

[0024] ① The molecular weight of the hollow fiber used for the concentration of foot-and-mouth disease virus is 50K, the pore size is 0.5mm,...

Embodiment 2

[0030] Embodiment 2: Foot-and-mouth disease O-type inactivated vaccine manufacture, inoculate BHK21 cell suspension culture with foot-and-mouth disease O-type virus OZK / 93 strain and OR / 80 strain respectively, harvest cell culture, after the inactivation through divinylimine (BEI) respectively , and then processed by the following methods, adding mineral oil adjuvant to mix and emulsify to make a vaccine, which is used to prevent swine type O foot-and-mouth disease.

[0031] (1) Use a continuous flow centrifuge to remove large particulate matter in the antigen

[0032] ①Use a continuous flow centrifuge with a set speed of 8000 rpm.

[0033] ② Collect the light liquid discharged from the centrifuge and discard the sediment (heavy liquid).

[0034] (2), process the antigen solution after the step (1) with a hollow fiber ultrafiltration system

[0035] ①The molecular weight specification of the hollow fiber used for the concentration of foot-and-mouth disease virus is 100K, the...

Embodiment 3

[0041] Embodiment 3: foot-and-mouth disease type O inactivated vaccine manufacture, inoculate BHK21 cell suspension culture with foot-and-mouth disease type O virus OZK / 93 strain and OR / 80 strain respectively, harvest cell culture, after being inactivated by divinylimine (BEI) respectively , and then processed by the following methods, adding mineral oil adjuvant to mix and emulsify to make a vaccine, which is used to prevent swine type O foot-and-mouth disease.

[0042] (1) Use a continuous flow centrifuge to remove large particulate matter in the antigen

[0043] ① Use a continuous flow centrifuge, set the speed at 5000 rpm.

[0044] ② Collect the light liquid discharged from the centrifuge and discard the sediment (heavy liquid).

[0045] (2), process the antigen solution after the step (1) with a hollow fiber ultrafiltration system

[0046] ①The molecular weight specification of the hollow fiber used for the concentration of foot-and-mouth disease virus is 300K, the pore...

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Abstract

The invention provides an effective novel method for foot-and-mouth disease viral antigen concentration and purification. The method comprises the following steps of: 1) eliminating large particle matters in the antigen by using a continuous flow centrifuge; 2) processing the antigen liquid after the step 1) by using a hollow fiber ultra-filtration system; and 3) processing the circulation liquid after the step 2) by using PEG6000 (polyethylene glycol 6000). By utilizing the method for foot-and-mouth disease vaccine concentration and purification, provided by the invention, both the concentration and purification of the foot-and-mouth disease viral antigen can be carried out; and the manufactured foot-and-mouth disease vaccine has the advantages of good immune effect and less adverse reaction.

Description

technical field [0001] The invention relates to a method for concentrating and purifying a foot-and-mouth disease virus vaccine. The combination of a continuous flow centrifuge, a hollow fiber and PEG can not only concentrate the foot-and-mouth disease antigen, but also purify the antigen. Background technique [0002] Foot-and-mouth disease is an acute, febrile, and highly contagious infectious disease of cloven-hoofed animals caused by foot-and-mouth disease virus. It is listed as the first class A infectious disease by the International Veterinary Office (OIE). Vaccination is the main means of controlling foot-and-mouth disease in most countries in the world. The vaccine manufacturing process is complex, especially the selection of the antigen treatment process is particularly important. The 146S antigen is the decisive antigen for the immunogenicity of the foot-and-mouth disease vaccine. It is relatively fragile and easily broken or cracked, which will lead to the loss o...

Claims

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Application Information

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IPC IPC(8): A61K39/135C07K14/09C07K1/36A61P31/14
Inventor 张翀宇李荣魏学锋徐师军任伟郭建军张龙陈九连
Owner JINYUBAOLING BIO PHARM CO LTD
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