Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Quantitative and large-scale infecting method for testing antiviral capability of prawns

An ability test, large-scale technology, applied in fish farming, application, climate change adaptation, etc., can solve problems such as excessive infection, cannibalization, trauma, etc., to avoid repeated infection, ensure integrity, and avoid ingestion of poisonous bait effects.

Inactive Publication Date: 2012-08-15
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to provide a quantitative and large-scale infection method for the test of the anti-virus ability of prawns, so as to prevent the cannibalization of the same kind of prawns and avoid excessive infection, so as to solve the problems caused by injection and extensive feeding during the current test of prawn disease resistance. Trauma, cannibalism of the same kind, excessive infection and other defects caused by other reasons, to ensure that complete and accurate information is provided for the breeding of excellent disease-resistant varieties

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Quantitative and large-scale infecting method for testing antiviral capability of prawns
  • Quantitative and large-scale infecting method for testing antiviral capability of prawns

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0015] The content of the method of the present invention will be further explained below in conjunction with the examples. In this example, Penaeus chinensis was used as the experimental object to test its disease resistance to white spot syndrome virus, and seedlings with strong resistance to WSSV were bred through continuous experiments.

[0016] In May 2006, 50 Chinese prawn families were established in the Genetic Breeding Center of the Yellow Sea Fisheries Research Institute of the Chinese Academy of Fishery Sciences. When the Chinese prawns grew to about 2.0cm, the visible fluorescent markers (visible implant elastomer, VIE) were carried out on the individuals of each family After marking, when the body length of Penaeus chinensis is 2.0-2.5cm, each family selects 30 tails to carry out the WSSV artificial infection experiment. The specific method steps are as follows:

[0017] 1) Preparations before infection Select Chinese prawns of various families with healthy physiq...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a quantitative and large-scale infecting method for testing antiviral capability of prawns, belonging to the field of select breeding of antiviral fine breeds of prawns. The quantitative and large-scale infecting method comprises the steps of preparing before infection, preparing an infection source, infecting prawns one by one, and collecting and analyzing data, wherein the step of infecting the prawns one by one comprises the following steps of: respectively putting the prawns to be tested in a transparent plastic container, wherein a black background is paved at the bottom of the plastic container so as to observe conveniently; and after hungering the prawns for 2 hours, feeding poison baits into the container, and observing the feeding condition of the prawns. According to the invention, the simple, convenient and independent transparent plastic container is utilized; when the large-scale process of testing the antiviral capability of the prawns is carried out, each prawn is put in the independent container and is fed for one time; the phenomenon, such as grabbing of the prawns and the like, can be effectively avoided; furthermore, the prawns can be prevented from eating poison baits for many times; influence of a test result caused by repeated infection and excess infection quantity can be avoided; and in addition, because prawn bodies are transparent and the black background is paved at the bottom of the container, feeding and eating observation are convenient.

Description

Technical field: [0001] The invention belongs to the field of prawn disease-resistant improved variety breeding, and in particular relates to a quantitative and large-scale infection method for testing the anti-virus ability of prawns. Background technique: [0002] Common diseases of prawns include white spot syndrome, taura virus disease, red body disease and red leg disease, etc. Shrimp diseases have always plagued the recovery and development of the prawn farming industry. Carry out artificial infection experiments to study the resistance of prawns to diseases, especially in the process of breeding disease-resistant improved varieties of prawns, it is necessary to carry out artificial infection experiments on large quantities of prawns, and record the individual or family of prawns after eating the bait with virus. Survival time is used to judge the resistance of each individual or family of prawns to the virus, and provide strong evidence for the selection of disease-re...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 孔杰张天时孟宪红罗坤
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com