Gomphrena globosa test tube flower and culture method thereof
A cultivation method and technology of Qianrihong, which are applied in the field of Qianrihong test tube flower and its cultivation, to achieve the effects of shortening vegetative growth time, strong practicability and good promotion.
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Embodiment 1
[0033] Embodiment one A method for cultivating a thousand day red test-tube flower, comprising the following steps:
[0034] 1. Cultivation of sterile seedlings: surface sterilize the seeds with 75% alcohol by volume for 30 minutes, sterilize them with 0.1% mercuric chloride for 10 minutes, soak them in sterile water for 4 times, and inoculate them into the sterile seedling medium; The temperature of the culture room is 26°C, the light is 12 h / d, and the light intensity is 1300 LX; the sterile seedling medium is: improved MS + 1 mg / L 6-BA + 0.2 mg / L NAA + 10 mg / L PP 333+20 g / L sucrose+6 g / L agar powder;
[0035] 2. Proliferation culture: Take the germination of the aseptic seedlings in step 1, cut them into 1 cm budded stem segments, inoculate them into the proliferation medium, and subculture once every 30 days; the temperature of the culture room is 28°C, and the light is 12 h / d. The light intensity is 1500 LX; the proliferation medium is: MS+0.5 mg / L 6-BA+0.5 mg / L NA...
Embodiment 2
[0039] Embodiment two A method for cultivating a thousand day red test-tube flower, comprising the following steps:
[0040] 1. Cultivation of sterile seedlings: Disinfect the surface of the seeds with 75% alcohol by volume for 30 minutes, sterilize them with 0.1% mercuric chloride for 10 minutes, soak them in sterile water for 3 times, and inoculate them into sterile seedling medium. The temperature of the culture room is 28°C, the light is 12 h / d, and the light intensity is 1200 LX; the sterile seedling medium is: improved MS + 1 mg / L 6-BA + 0.2 mg / L NAA + 10 mg / L PP 333 +20 g / L sucrose+6 g / L agar powder;
[0041] 2. Proliferation culture: take the sprouts from step 1, cut the 1cm-long stems with buds, and inoculate them into the proliferation medium; subculture once every 30 days; the temperature of the culture room is 28°C, the light is 12 h / d, and the light intensity is 1300 LX ; The proliferation medium is: MS+0.5 mg / L6-BA+0.5 mg / LNAA+20 g / L sucrose+6 g / L agar po...
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