Poultry egg windowing and sealing method

Abstract

The invention discloses a poultry egg windowing and sealing method. The invention comprises the steps: windowing on a poultry egg equatorial plate position, keeping a full eggshell membrane, forming a concentric sealing ring on the eggshell by using hot melt adhesive or the like, taking a window center as a circle center, dropwise adding physiological buffer solution after the concentric sealing ring and the eggshell surface are bonded firmly, removing the eggshell membrane under the buffer solution blockage condition, positioning an embryo and performing the embryo operation, then fully covering the window under the solution by using the prepared eggshell membrane, absorbing surplus solution in the sealing ring by using absorbent paper, then filling the interior of the sealing ring by using the hot melt adhesive or gelatin. The poultry egg windowing and sealing method effectively increases transgenic poultry egg hatching rate and transgenic efficiency by optimizing an operation proposal.

Description

technical field [0001] The invention belongs to the field of transgenic technology, and relates to a method for opening and sealing poultry eggs. Background technique [0002] Poultry transgenic is of great significance for obtaining animal models of specific genotypes and producing therapeutically valuable medicinal proteins, and has huge scientific and commercial potential value. Due to the special reproductive and physiological characteristics of poultry and the limitation of technical conditions, the most commonly used and most practical method is to conduct genetically modified operations on poultry eggs. Manipulation of poultry eggs necessarily involves opening the shell and shell membrane to gain access to the embryo. It can be divided into two cases. One is the method of changing egg shells, that is, the manipulated poultry embryo egg is relatively small, and the egg shell is opened at the blunt end. For example, after transgenic operation with chicken embryos, mov...

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Problems solved by technology

But there are also some obvious problems in this method: as, when dripping liquid to cover the opening, the amount of dripping liquid is not easy to control, and if there is too much dripping, it will easily flow and cause the failure of sealing air, and if the dripping is less, it will lose the effect of blocking air entry (and most Eggs will absorb more liquid), and the shape of different eggs varies greatly, so it is difficult to have a unified standard; moreover, some eggshell surfaces are often hydrophilic, and it is impossible to form droplets to achieve the purpose of sealing. Applying hydrophobic substances such as paraffin oil on the surrounding area has a certain effect, but it is still inconvenient to operate, especially after applying hydrophobic substances, it often affects the final sealing effect, resulting in weak sealing and easy falling off, resulting in hatching failure

However, the disadvantages of this method are also obvious. The most prominent problem is that since the solidification point of the low-melting hot melt adhesive is generally at 60-80°C, it will solidify quickly below this temperature, leaving room for embryo positioning under normal room temperature operating conditions. The time for operating and exhausting the gas is very limited (and this is often time-consuming), which often leads to confusion and a low success rate of operation

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