Pollution-free culture method for cordyce militaris
A technology of Cordyceps militaris and its cultivation method, which is applied in the field of pollution-free cultivation of Cordyceps militaris, and can solve the problems of few wild resources of Cordyceps militaris, limited sources of natural medicinal materials, and large dosage.
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[0005] The preferred embodiments of the present invention will be described in detail below; it should be understood that the preferred embodiments are only for illustrating the present invention, rather than limiting the protection scope of the present invention.
[0006] Activation of strains: The slant strains stored in the refrigerator were activated by solid culture method or liquid fermentation method, and the activated strains were obtained by static culture at 25 °C for 48 hours; the solid culture method was prepared according to the formula of PDA comprehensive medium, and after high-pressure sterilization Prepare the slant, insert the preserved strains into the newly prepared medium, and culture the grafted strains in a 25°C incubator until the white mycelium covers the slant of the test tube; the liquid fermentation method uses the PDA slant medium The activated Cordyceps militaris was added to the above five liquid media at 25°C, 130 r min -1 and 12 h of light for ...
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