Curettage medicine composition for dairy cattles
A technology for clearing the palace and medicine, applied in the direction of medicine combination, medicine formula, plant raw materials, etc., can solve problems such as antibiotic resistance, antibiotic residue, and ineffective treatment
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[0012] 1.4 Preparation of isolated uterus 2 days before the experiment, subcutaneous injection of diethylstilbestrol 0.2mg / only every day, to improve the sensitivity of uterine smooth muscle, according to the literature (Chen Qi, Chinese Medicine Pharmacological Research Methodology, Second Edition Beijing. Agricultural Press. 1987, 568-569.) Preparation of isolated uterus.
[0013] 1.5 Tracing of the isolated mouse uterus The two ends of the uterine horn were ligated with silk thread, and then one end was fixed on the "L"-shaped ventilation hook of the McFurney bath dish, and the other end was connected to the muscle tension transducer. After the uterine contractions are stable, record and observe the indicators. First observe the voluntary contraction of the isolated uterine smooth muscle under normal conditions. After stabilization, add different doses of the test drugs to the McFarland bath. The uterine smooth muscle contraction force (g) is used as the index to record t...
Embodiment 2
[0028] 1 Materials and methods
[0029] 1.1 The test cows were Chinese Holstein dairy cows from Sichuan Agricultural University Dairy Farm and Ya'an Sanxing Dairy Farm. 20 dairy cows diagnosed with acute endometritis were selected as the experimental group, and 5 healthy dairy cows were selected as the clinical examination. Clinical healthy control group.
[0030] 1.2 Medicines and instruments The palace-clearing medicine combination of the present invention is prepared into an aqueous solution containing 1 g crude drug per milliliter by decoction, NEX-4B laminar blood viscometer, Wen's hematocrit tube, and long needle for flushing.
[0031] 1.3 Blood sample collection The experimental group was randomly divided into two groups, with 10 cows in each group. In group I, each cow was given 250ml of Qinggong drug combination, once a day, for 3 days. The cows in group II did not need any treatment, and the cows in group I were treated before and after treatment. After (1d after th...
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