Curettage medicine composition for dairy cattles
A technology for clearing palaces and medicines, applied in the direction of drug combinations, medical formulas, plant raw materials, etc., can solve problems such as antibiotic resistance, antibiotic residues, and ineffective treatment
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[0012] 1.4 Preparation of isolated uterus Subcutaneously inject 0.2 mg / day of diethylstilbestrol every day before the experiment to improve the sensitivity of uterine smooth muscle, according to the literature (Chen Qi, Chinese Medicine Pharmacological Research Methodology, Second Edition Beijing. Agricultural Press. 1987, 568-569.) Preparation of isolated uteri.
[0013] 1.5 Tracing of the isolated mouse uterus The two ends of the uterine horn were ligated with silk thread, and then one end was fixed on the "L"-shaped ventilation hook of the Maxwell dish, and the other end was connected to the muscle tone transducer. After the uterine contraction is stable, record and observe the indicators. First observe the voluntary contraction of the isolated uterine smooth muscle under normal conditions. After stabilization, add different doses of the test drug into the Maxwell bath one by one, and use the uterine smooth muscle contraction force (g) as an index to record the changes in t...
Embodiment 2
[0028] 1 Materials and methods
[0029] 1.1 Cows to be tested were Chinese Holstein cows from the Dairy Farm of Sichuan Agricultural University and Ya’an Sanxing Dairy Farm. 20 dairy cows diagnosed with acute endometritis were selected as the test group, and 5 healthy dairy cows were selected as the experimental group. Clinical healthy control group.
[0030] 1.2 Medicines and instruments The medicine combination for clearing the uterus of the present invention is prepared into an aqueous solution containing 1 g of crude drug per milliliter by decoction with water for standby use, NEX-4B vertebral plate blood viscosity meter, Wen's hematocrit tube, and long needle for flushing.
[0031] 1.3 The blood sample collection test group was randomly divided into two groups, with 10 cows in each group. Each cow in group I was given 250ml of uterine-clearing drug combination, once a day, for 3 days in a row; Afterwards (1 day after the last medication), blood was collected from the jug...
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