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Ocean plankton size-spectra in-situ detection instrument

A plankton and detector technology, applied in measurement devices, particle size analysis, scientific instruments, etc., can solve the problem of affecting the accuracy of particle size spectrum information, inability to obtain marine ecosystem particle size spectrum information on-site, and heavy data processing workload. problems, to achieve the effect of eliminating post-data processing and realizing real-time on-site detection

Inactive Publication Date: 2012-07-18
XIAMEN UNIV
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Problems solved by technology

However, existing instruments and equipment, such as flow imaging and microscopic imaging systems (FlowCAM, Fluid-Imaging Technologies, Inc., USA), only store image information and fluorescence information, and require manual identification of image information. The data processing workload is heavy, and the particle size spectrum information of the marine ecosystem cannot be obtained on site; at the same time, the particle size calculation error is large, and the particle size is only obtained by converting the pixel area, which seriously affects the accuracy of the particle size spectrum information

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  • Ocean plankton size-spectra in-situ detection instrument
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  • Ocean plankton size-spectra in-situ detection instrument

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specific Embodiment

[0025] 1. Collection of marine plankton image information and fluorescent signals:

[0026] In the present invention, the plankton cells in the sample pool 1 are driven by the peristaltic pump 3 to reach the flow chamber 2 . In the flow chamber 2 , the plankton cells are irradiated by the LED imaging light source 4 , and their image information is collected by the CCD camera 15 . During this process, the optical magnification of the objective lens 5 and the eyepiece 12 and the digital magnification of the CCD camera 15 enable the image information of the plankton to be properly magnified to meet the requirements of subsequent image processing.

[0027] At the same time, plankton cells are excited by the laser light emitted by the laser 14 in the process of passing through the flow chamber 2 to generate various fluorescent signals. Wherein part of the fluorescence signal reaches the beam splitter 8 under the convergence of the objective lens 5, and under the action of the beam...

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Abstract

The invention relates to an ocean plankton size-spectra in-situ detection instrument, which relates to ocean plankton detection equipment. The ocean plankton size-spectra detection instrument is provided with a flow-path system, an image system, an optical-path system and a control circuit; the flow-path system is provided with a sample pond, a flow chamber and a peristaltic pump; the image system is provided with a light emitting diode (LED) shooting light source, an object lens, a second spectroscope, an ocular and a charged coupled device (CCD) camera; the optical-path system is provided with a laser, a light beam shaping device, a first spectroscope, a third spectroscope, a filter plate, a first fluorescent detector, a filter plate and a second fluorescent detector; and the control circuit is connected with the peristaltic pump in the flow-path system, the LED shooting light source and the CCD camera in the image system and the first and the second fluorescent detectors in the optical-path system.

Description

technical field [0001] The invention relates to a detection device for marine plankton, in particular to an on-site detector for particle size spectrum of marine plankton. Background technique [0002] Each species in the marine ecosystem forms a complex food web through feeding relationships, and it is difficult to accurately describe the entire ecosystem using traditional methods (Zhou Linbin, Tan Yehui, Huang Liangmin, et al. Aquatic particle size spectrum / biomass spectrum Research progress [J]. Acta Ecologica Sinica, 2010, (12): 3319-3333; Wang Xingang, Sun Song. Application of particle size spectrum theory in marine ecology research [J]. Marine Science, 2002, (4): 36 -39). The particle size spectrum theory classifies plankton according to particle size, establishes a simple correspondence between plankton biomass and particle size in the same curve, simplifies the food web, and becomes an important means to describe the marine ecosystem . The optical microscope metho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/02
Inventor 骆庭伟焦念志
Owner XIAMEN UNIV
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