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Method for producing erythrocin at high yield

An erythromycin, high-yield technology, applied in the field of microbial fermentation, can solve the problems of difficulty in online detection, connection of optimization control, influence of erythromycin production, etc., to achieve the effect of improving the fermentation level

Active Publication Date: 2012-07-18
ANHUI BBCA FERMENTATION TECH ENG RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the difficulty of online detection such as the determination of intermediate metabolites and enzyme activity in the fermentation process in the bioreactor, it is impossible to link these results with the optimization control of the ongoing fermentation process, and the fermentation process can only stay at the optimal state of static optimization. groping for the best conditions
In addition, during the microbial fermentation process, the growth and metabolism of the bacteria in the early stage of fermentation will have a greater impact on the entire fermentation process and the final erythromycin production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] 1) Slant culture: Saccharopolyspora erythraea CGMCC4.197 was inoculated on a solid slant medium under sterile conditions, and cultured at a constant temperature of 33° C. for 8-10 days.

[0012] 2) Inoculate the bacteria cultured in step 1) into a fermentation bottle under aseptic conditions, and culture at a constant temperature of 33°C and 300r / min for 8 days.

[0013] Wherein step 1) the slant medium formula that uses: starch 5g / L, glucose 10g / L, corn steep liquor 1g / L, ZnSO 4 .7H 2 O 0.1g / L, MnCl 2 .4H 2 O 0.02g / L, agar 15g / L, prepared with water, used after sterilization.

[0014] Wherein step 2) the fermentation medium formula that uses: starch 30g / L, soybean meal powder 40g / L, soybean oil 7g / L, ammonium sulfate 3g / L, zinc sulfate 0.1g / L, calcium carbonate 20g / L, with water Prepared and used after sterilization.

[0015] Fermentation culture process: under the condition of 33°C, 300r / min, constant temperature culture for 8 days, the average fermentation level...

Embodiment 2

[0017] 1) Slant culture: Saccharopolyspora erythraea CGMCC4.197 was inoculated on a solid slant medium under sterile conditions, and cultured at a constant temperature of 33° C. for 8-10 days.

[0018] 2) Inoculate the bacteria cultured in step 1) into a fermentation bottle under aseptic conditions, and culture at a constant temperature of 33°C and 300r / min for 8 days.

[0019] Wherein step 1) the slant medium formula that uses: starch 5g / L, glucose 10g / L, corn steep liquor 1g / L, ZnSO 4 .7H 2 O 0.1g / L, MnCl 2 .4H 2 O 0.02g / L, agar 15g / L, prepared with water, used after sterilization.

[0020] Wherein step 2) the fermentation medium formula that uses: starch 30g / L, soybean meal powder 40g / L, soybean oil 7g / L, ammonium sulfate 3g / L, zinc sulfate 0.1g / L, calcium carbonate 20g / L, with water Prepared and used after sterilization.

[0021] Fermentation and cultivation process: under the condition of 33°C, 300r / min, ferment for 48 hours, replenish 5% water, cultivate at constant...

Embodiment 3

[0023] 1) Slant culture: Saccharopolyspora erythraea CGMCC4.197 was inoculated on a solid slant medium under sterile conditions, and cultured at a constant temperature of 33° C. for 8-10 days.

[0024] 2) Inoculate the bacteria cultured in step 1) into a fermentation bottle under aseptic conditions, and culture at a constant temperature of 33°C and 300r / min for 8 days.

[0025] Wherein step 1) the slant medium formula that uses: starch 5g / L, glucose 10g / L, corn steep liquor 1g / L, ZnSO 4 .7H 2 O 0.1g / L, MnCl 2 .4H 2 O 0.02g / L, agar 15g / L, prepared with water, used after sterilization.

[0026] Wherein step 2) the fermentation medium formula that uses: starch 30g / L, soybean meal powder 40g / L, soybean oil 7g / L, ammonium sulfate 3g / L, zinc sulfate 0.1g / L, calcium carbonate 20g / L, with water Prepared and used after sterilization.

[0027] Fermentation and cultivation process: under the condition of 33°C, 300r / min, ferment for 72 hours, replenish 10% water, cultivate at constan...

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Abstract

The invention provides a method for producing erythrocin at high yield. In the method, Saccharopolyspora erythraea CGMCC (China General Microbiological Culture Collection Center) 4.197 is taken as a fermentation strain, dissolved oxygen is improved through water supplementation, and a fermentation level of the erythrocin is improved; and experiment proves that when water starts to be supplemented after fermentation is carried out for 30-100h and the water supplementation amount is 5-30% of the volume of fermentation liquid, the defects that oxygen supply is not enough duding the fermentation due to overhigh concentration of strains are improved, and the average fermentation level is improved from 600u / ml to 2000u / ml. The method for producing the erythrocin at high yield, provided by the invention is simple and feasible and has positive reference significance to other types of high- oxygen-consumption fermentation.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a method for high-yielding erythromycin by improving fermentation conditions of bacterial strains. Background technique [0002] Erythromycin is a macrolide antibiotic with an antimicrobial spectrum similar to that of penicillin G. It is commonly used clinically for patients allergic to penicillin. Due to the widespread use of erythromycin and its derivatives in recent years, the demand for erythromycin has increased significantly. Although my country started industrial production of erythromycin in the 1960s, the fermentation level is not high, and there is a large gap compared with developed countries, and the production profit is very low. [0003] In recent years, due to the continuous discovery of curative effects and functions of semi-synthetic drugs such as clarithromycin and azithromycin derivatives of erythromycin, the demand for erythromycin raw materials in the m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/62C12R1/645
Inventor 李荣杰
Owner ANHUI BBCA FERMENTATION TECH ENG RES
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