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Water-soluble polysaccharide extracted from black fungus and preparation method thereof

A technology of water-soluble polysaccharide and black fungus is applied in the fields of natural macromolecules and food to achieve the effects of low cost, improved immunity and easy gelation

Inactive Publication Date: 2012-07-18
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on black fungus polysaccharides in domestic and foreign literature is limited to water-soluble heteropolysaccharides and acidic polysaccharides, and the water-soluble β-(1→3)-D-glucan with higher biological activity involved in the present invention has not yet been reported.

Method used

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  • Water-soluble polysaccharide extracted from black fungus and preparation method thereof
  • Water-soluble polysaccharide extracted from black fungus and preparation method thereof
  • Water-soluble polysaccharide extracted from black fungus and preparation method thereof

Examples

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Effect test

Embodiment 1

[0020] Purchase commercially available black fungus fruiting bodies from Fang County, Hubei Province, degrease the dried black fungus fragments with ethyl acetate and acetone for 4 hours, and then soak the dried fragments in a mixed solvent of ethanol / water with a volume ratio of 70 / 30 overnight. Separate the residue, soak in 0.9wt% NaCl aqueous solution, stir at 100°C for 2-4 hours, then continue to stir at room temperature for 20-22 hours, centrifuge, collect the supernatant, repeat this step three times; 30wt%H 2 o 2 Decolorize to light yellow, remove free protein with Sevag method, this method is based on the characteristics of protein denaturation in organic solvents such as chloroform, mix chloroform and n-butanol mixture (its volume ratio is 5: 1) with polysaccharide solution, vigorously After shaking for 20 minutes, centrifuge to remove the denatured protein at the junction of the water layer and the solvent layer. Repeat 6-10 times until there is no protein absorpt...

Embodiment 2

[0034] Purchase commercially available black fungus fruiting bodies produced in Fang County, Hubei Province, perform Soxhlet extraction on the dried black fungus fragments with ethyl acetate and acetone for 4 hours each, and then soak in 70vol% ethanol aqueous solution overnight. The residue was extracted with 0.9% NaCl aqueous solution at 70°C for 2-4 hours, then extracted at room temperature until 20-22 hours, centrifuged, and the supernatant was collected. This step was repeated three times; the supernatant was extracted with 30wt% H 2 o 2Decolorize to light yellow, remove free protein by Sevag method, repeat 6-10 times until there is no protein absorption peak at 280nm by UV detection. Then, tap water and distilled water were respectively dialyzed through a regenerated cellulose membrane dialysis bag (Mw cut-off8000, Union Carbide, NJ, USA) for 5 days and 3 days, concentrated, and freeze-dried to obtain black fungus crude polysaccharide. This crude polysaccharide contains...

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Abstract

The invention relates to a water-soluble polysaccharide extracted from black fungus and preparation method of the water-soluble polysaccharide. The main chain of the water-soluble polysaccharide is beta-(1->3)-D-glucan; 51-70% of main chain structure unit is connected with a (1->6) bonded glucose side group; the weight-average molecular weight is 400 thousand to 3 million; and the water-soluble polysaccharide is in expanded rigid single chain in water. The preparation method comprises the steps of: cutting dry black funguses; removing the fat; dipping in mixed solvent of ethanol / water, extracting the residue with normal saline at 70-100 DEG C, and centrifuging; decoloring the supernatant; removing the dissociative protein; dialyzing, concentrating, and carrying out freeze drying so as to obtain a crude polysaccharide of black fungus; redissolving the crude polysaccharide into water; precipitating and isolating with ethanol, and centrifuging; dissolving the precipitate in water; and dialyzing and carrying out freeze drying so as to obtain a water-soluble neutral polysaccharide. The prepared water-soluble polysaccharide has extremely high viscosity at a dilute solution state, is easy to gel and can be used for preparing efficient thickeners for foodstuff or health care products.

Description

technical field [0001] The invention relates to a water-soluble polysaccharide extracted from black fungus and a preparation method thereof, which belongs to the field of natural polymers and also belongs to the field of food technology. Background technique [0002] Natural polysaccharides have potential commercial uses due to their safety, special physicochemical properties, and biological activities, such as adjuvants and additives in the food industry and pharmaceutical industry. In particular, some water-soluble polysaccharides with rigid chains have high viscosity, are easy to form gels, and have immunomodulatory functions on the human body, such as triple-helical polysaccharides Schizophyllan and lentinan. Black fungus is a plant of the fungus Basidiomycetes, Auriculariaceae, and is a special product resource in my country. At present, the research on black fungus polysaccharides in domestic and foreign literature is limited to water-soluble heteropolysaccharides and...

Claims

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Application Information

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IPC IPC(8): C08B37/02A61P37/04A23L1/052A23L1/09A23L29/206
Inventor 张俐娜许淑琴许小娟
Owner WUHAN UNIV
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