Application of Stenotrophomonas maltophilia DS4 for degrading organic pollutants in saponin waste water

A technique for oligotrophic monotrophic bacteria and organic pollutants, which is applied in the application field of oligotrophic monotrophic bacterium DS4 in the degradation of organic pollutants in saponin wastewater, and can solve the problem of lack of oligotrophic oligotrophic bacterium DS4 and the like. , to achieve the effect of widening application, good ability to remove organic pollutants in saponin wastewater, and strong growth ability

Inactive Publication Date: 2012-07-18
CHINA UNIV OF GEOSCIENCES (WUHAN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, physical or chemical methods are mostly used for the treatment of saponin wastewater, and there is no report of Stenotrophomonas maltophilia DS4 and its application in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The screening method of embodiment 1 Stenotrophomonas maltophilia DS4

[0014] (1) Material preparation:

[0015] 1. The experimental wastewater and pool bottom sludge were taken from the biochemical treatment system of a saponin production plant in Shiyan City, Hubei Province.

[0016] 2. Culture medium:

[0017] Separation medium: K 2 HPO 4 0.5g / L, MgSO 4 ·7H 2 O 0.5g / L, NH 4 Cl 1.0g / L, CaCl 2 0.01g / L, KH 2 PO 4 0.5g / L, glucose 0.5g / L, pH 7.0.

[0018] Screening medium: K 2 HPO 4 0.5g / L, MgSO 4 ·7H 2 O 0.5g / L, NH 4 Cl 1.0g / L, CaCl 2 0.01g / L, KH 2 PO 4 0.5g / L, dissolved in 1L saponin wastewater, pH 7.0.

[0019] LB liquid medium: peptone 10.0g / L, yeast extract 5.0g / L, sodium chloride 10.0g / L, distilled water 1000mL, pH7.0.

[0020] LB solid medium: peptone 10.0g / L, yeast extract 5.0g / L, sodium chloride 10.0g / L, distilled water 1000mL, agar 15g / L (solid), pH7.0.

[0021] LB slant medium: peptone 10.0g / L, yeast extract 5.0g / L, sodium chloride 10.0...

Embodiment 2

[0035] Example 2 Application of Stenotrophomonas maltophilia DS4

[0036] A single colony of Stenotrophomonas maltophilia DS4 was picked and cultured overnight in LB solid medium. According to the cultured Stenotrophomonas maltophilia DS4 and saponin wastewater (that is, the experimental wastewater, it was taken from a certain site in Shiyan City, Hubei Province). The biochemical treatment system for wastewater discharged from a saponin production plant) is inoculated with a volume ratio of 0.5-2:100, cultivated at a constant temperature of 30-35°C, with a pH value of 6-8, and reacted for 48-72 hours.

[0037] The LB solid medium is: 10.0 g of peptone, 5.0 g of yeast extract, 10.0 g of NaCl, 1000 mL of distilled water, 15 g of agar (solid), pH 7.0.

[0038] The degradation efficiency of the screened microorganisms to organic pollutants in saponin wastewater was measured.

[0039] Pick a single colony, culture overnight in the isolation medium, take 1mL of the bacterial liquid...

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PUM

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Abstract

The invention discloses the application of Stenotrophomonas maltophilia Hilia DS4 for degrading organic pollutants in saponin waste water. The Stenotrophomonas maltophilia Hilia DS4 strain is preserved in China Center for Type Culture Collection with preservation number CCTCC No. M2010147. The Stenotrophomonas maltophilia Hilia DS4 strain has strong capability of growing in high-concentration sulfate ion environments and good ability of removing organic pollutants from saponin waste water. The strain can reduce the COD (chemical oxygen demand) of the saponin waste water by 2930 mg / L within 72 hours, with high removal efficiency up to 62.34%. The invention provides a useful bacterial source for degrading high-concentration organic pollutants in saponin waste water, increases the application range of Stenotrophomonas maltophilia Hilia, and has a high application value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the application of stenotrophomonas maltophilia DS4 in degrading saponin wastewater organic pollutants. Background technique [0002] The wastewater produced during the production of saponin (such as diosgenin) has high chroma, high acidity, high concentration of organic pollutants, high chemical oxygen demand (COD), and poor biodegradability. It is a very difficult wastewater to treat. "Saponin Industrial Water Pollutant Discharge Standard" (GB 20425-2006) stipulates that COD emissions should be less than 300mg / L since 2009. However, the current COD concentration of saponin hydrolysis stock solution reaches 20000-40000mg / L The COD concentration of wastewater is 4000-12000mg / L. If it is directly discharged into the water body, the concentration of organic pollutants in the water body will increase significantly, which will cause serious pollution of rivers and lakes, which not only dir...

Claims

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Application Information

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IPC IPC(8): C02F3/34C02F103/36
Inventor 郑丹鲍建国
Owner CHINA UNIV OF GEOSCIENCES (WUHAN)
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