System for treating sewage and producing biological oil by using microalgae and method
A bio-oil and sewage technology, applied in energy wastewater treatment, chemical instruments and methods, biological water/sewage treatment, etc., can solve the problems of limited photobioreactor development, achieve an excellent algae cell growth environment, convenient operation, The effect of uniform distribution of bubbles
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Embodiment 1
[0080] The common f / 2 medium (pH 7.0) for green algae was used for seed liquid culture in shake flasks, and sewage medium (pH 7.0) was used for seed tank culture and photobioreactor 1 culture. The sewage medium is the secondary effluent treated by the sewage treatment plant after the municipal sewage and industrial sewage are filtered out to remove bacteria or algae (total nitrogen is 30.0-50.0mg / L, ammonia nitrogen is 10.0-16.0mg / L, total phosphorus Concentration of 2.0 ~ 4.0mg / L) formed medium, through the feed port / inoculation port 19 into the photobioreactor 1 and sterilized, the initial inoculation density of 6.0 × 10 7 cfu / ml, stirring speed is 120rpm, light intensity is 15000lux, light cycle is 16h:8h, culture temperature is 30℃, pH is 7.0, CO 2 gas CO 2 The concentration is 30% (v / v), the ventilation rate is 0.1VVm, and the algal concentration in the photobioreactor 1 is as high as 2.0×10 before split culture and after 48 hours of culture. 8 cfu / ml, and the content...
Embodiment 2
[0084]Using continuous fermentation and using municipal sewage and industrial sewage after filtering out miscellaneous bacteria or miscellaneous algae, the secondary effluent treated by the sewage treatment plant as the medium, inoculate the medium with Chlorella C9-JN2010, wherein, the initial The connection density is 3.5×10 7 cfu / ml, the light cycle is 14h:10h, the light intensity is 5000lux, the culture temperature is 25°C, the pH is 8.5, CO 2 gas CO 2 Concentration is 25.0% (v / v), ventilation rate is 0.2VVm, continuous culture 480h, nitrogen, phosphorus removal effect, CO 2 The conversion rate (% (v / v)) and oil accumulation relationship see Figures 7A-7B shown.
[0085] Depend on Figure 7A The analysis shows that in the early stage of continuous culture, as the culture time prolongs, its response to nitrogen, phosphorus and CO 2 utilization is accelerated. From 0 to 120 hours, the utilization rate of nitrogen increased continuously, and then the removal rate reach...
Embodiment 3
[0088] The municipal sewage and industrial sewage after filtering out miscellaneous bacteria or miscellaneous algae by adopting batch fermentation method are used as the culture medium through the secondary effluent of the sewage treatment plant, and the culture medium is inoculated with Chlorella C9-JN2010, wherein, the initial inoculation Density is 1.0×10 7 cfu / ml, the light cycle is 10h:14h, the light intensity is 7500lux, the culture temperature is 20°C, the pH is 6.5, CO 2 The concentration is 15.0% (v / v), the ventilation rate is 0.01VVm, and the batch culture is 480h, the removal effect of nitrogen and phosphorus, CO 2 The conversion rate (% (v / v)) and oil accumulation relationship see Figures 8A-8B shown.
[0089] Depend on Figure 8A The analysis shows that in the early stage of culture, with the extension of culture time, its response to nitrogen, phosphorus and CO 2 utilization is accelerated. The utilization rate of nitrogen continued to increase within 0-168...
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