Method for preparing cross-linked collagen/chitosan tissue engineering porous support in one-step freeze-drying mode

A porous scaffold and tissue engineering technology, which is applied in the field of preparing cross-linked collagen/chitosan tissue engineering porous scaffold by one-time freeze-drying, can solve the problem of unfavorable collagen/chitosan porous scaffold large-scale preparation process stabilization, unfavorable collagen /The industrial production of chitosan porous scaffolds, large batch quality differences and other issues, to achieve the effect of large-scale preparation, avoiding scaffold collapse and microstructure damage, and stable scaffold performance

Active Publication Date: 2012-07-18
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Because this method involves two freeze-drying processes, it is prone to problems such as surface collapse of the scaffold and internal microstructure damage, and the traditional preparation method also has disadvantages such as cumbersome preparation process, high energy consumption, long time consumption, and large batch quality variance. It is conducive to the large-scale preparation of collagen / chitosan porous scaffolds and the stabilization of the preparation process, and it is not conducive to the industrial production of collagen / chitosan porous scaffolds

Method used

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  • Method for preparing cross-linked collagen/chitosan tissue engineering porous support in one-step freeze-drying mode
  • Method for preparing cross-linked collagen/chitosan tissue engineering porous support in one-step freeze-drying mode
  • Method for preparing cross-linked collagen/chitosan tissue engineering porous support in one-step freeze-drying mode

Examples

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example 1

[0025] A method for preparing a cross-linked collagen / chitosan tissue engineering porous scaffold by freeze-drying once, comprising the following steps:

[0026] (1) Prepare beef tendon collagen solution and chitosan solution with a mass concentration of 0.5% in acetic acid solution, mix the beef tendon collagen solution and chitosan solution at a volume ratio of 9:1, and stir evenly to obtain beef tendon collagen / chitosan mixed swelling solution. Inject glutaraldehyde (GA) solution with a mass concentration of 2.5% into the mixed swelling solution to make the cross-linking concentrations respectively 0.04%, 0.1%, and 0.25%, and then at 37 oC The reaction was stirred for 4 hours. Inject the reacted bovine tendon collagen / chitosan mixed swelling solution into the mold at -20 oC After freezing for 2 hours, lyophilize to obtain a cross-linked bovine tendon collagen / chitosan porous scaffold. The cross-linking concentration refers to the mass concentration of glutaraldehyde i...

example 2

[0033] A method for preparing a cross-linked collagen / chitosan tissue engineering porous scaffold by freeze-drying once, comprising the following steps:

[0034] (1) Prepare collagen solution and chitosan solution with a mass concentration of 0.5% in acetic acid solution, mix the collagen solution and chitosan solution at a volume ratio of 9:1, and stir evenly to obtain collagen / chitosan Sugar mixed swelling solution. Inject the glutaraldehyde solution of mass concentration 2.5% in this mixed swelling liquid, make cross-linking concentration be 0.04%, then at 37 oC The reaction was stirred for 4 hours. Inject the reacted collagen / chitosan mixed swelling solution into the mold at -20 oC After freezing for 2 hours, lyophilize to obtain a cross-linked collagen / chitosan porous scaffold. The cross-linking concentration refers to the mass concentration of glutaraldehyde in the mixed swelling solution, using the formula 2.5% × V GA = C×(V GA +V 1 ) calculation, where V GA Re...

example 3

[0037] A method for preparing a cross-linked collagen / chitosan tissue engineering porous scaffold by freeze-drying once, comprising the following steps:

[0038] (1) Prepare fish skin collagen solution and chitosan solution with a mass concentration of 0.5% in hydrochloric acid solution, mix the fish skin collagen solution and chitosan solution at a volume ratio of 9:1, and stir evenly to obtain fish skin collagen / chitosan mixed swelling solution. Inject the glutaraldehyde solution of mass concentration 2.5% in this mixed swelling liquid, make cross-linking concentration be 0.1%, then in 37 oC The reaction was stirred for 4 hours. Inject the reacted fish skin collagen / chitosan mixed swelling solution into the mold at -20 oC After freezing for 3 hours, freeze-dry to obtain a cross-linked fish skin collagen / chitosan porous scaffold. The cross-linking concentration refers to the mass concentration of glutaraldehyde in the mixed swelling solution, using the formula 2.5% × V ...

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Abstract

The invention discloses a method for preparing a cross-linked collagen / chitosan tissue engineering porous support in one-step freeze-drying mode, which uses natural biological material of collagen and chitosan as raw materials, directly adds collagen / chitosan mixed swelling solutions in a glutaraldehyde solution and prepares the collagen / chitosan tissue engineering porous support through a one-step refrigeration freeze-drying mode. The collagen / chitosan support prepared through the method has the advantages of being controllable in mechanical property, proper in degradation rate and good in biocompatiability. Further, the method only needs a one-step freeze-drying process and effectively solves the problems of support collapsing, micro-structure breaking and the like caused by a traditional preparing method. The method is simple and practical, low in energy consumption, time-saving, material-saving and good in repeatability, and the constructed collagen / chitosan porous support can be widely used in the field of tissue engineering and has good clinical application prospect.

Description

technical field [0001] The invention relates to a preparation method of a tissue engineering porous support, in particular to a method for preparing a cross-linked collagen / chitosan tissue engineering porous support by one-time freeze-drying. Background technique [0002] The skin is the largest organ of the human body. Due to its large area exposed to the external environment, it will inevitably be subject to various injuries such as wounds, burns, scalds, etc. The ability of self-repair after skin injury is limited. The common clinical treatment methods include autologous skin transplantation and allogeneic skin transplantation. However, due to the shortage of donors and immune rejection, it is difficult to meet the clinical needs. With the development of tissue engineering and regenerative medicine, tissue engineered skin has been widely concerned and recognized. How to construct a skin with controllable composition and microstructure, suitable degradation rate and mechan...

Claims

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Application Information

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IPC IPC(8): A61L27/56A61L27/24A61L27/20
Inventor 马列高长有刘云云
Owner ZHEJIANG UNIV
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