Rearing method of non-toxic brown citrus aphids for researching virus transmission molecular mechanism
A molecular mechanism, brown technology, applied in animal husbandry and other directions, can solve the problems of different and little progress in the molecular mechanism of aphid transmission, and achieve the effect of simple production, no influence on the transmission efficiency, and easy preparation.
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Embodiment 1
[0019] (1) subculture of non-toxic brown orange aphid on citrus seedlings
[0020] Citrus aphids are collected from the field, and a non-toxic population is established with newly hatched nymphs (nymphs refer to newly born aphids, and newly hatched nymphs refer to newly born aphids that have not touched the leaf surface), and detoxified orange seedlings or seedlings Raising brown citrus aphids, in order to ensure that there are always tender aphids, pruning 20 non-toxic citrus seedlings every week in a greenhouse with a temperature of 25±2°C, and applying 0.5% urea or compound fertilizer to promote hair tips after pruning. Faxin later moved to the insect culture room. The temperature in the insect culture room was set at 25±2°C, and the light:dark ratio was 14:10h. Gently place more than 30 wingless adults on the new shoots of non-toxic citrus seedlings to produce nymphs. After 1-2 days, transfer the adults to another non-toxic seedling. The next generation of 1-2 day-old wing...
Embodiment 2
[0038] The other steps of this embodiment are exactly the same as in Embodiment 1, the difference has the following two points:
[0039] (1) is the preparation of feeding solution: NaCl 8g, KCl 0.1g, NaCl 2 HPO 4 12H 2 O 3g, KH 2 PO 4 0.1g, 50g of yeast extract powder, adjust the pH to 7.0, make 0, 10%, 20%, 25%, 30%, 35%, 40%, 50% and 60% different sucrose concentration feeding solutions, use deionized Prepare with water, and set the volume to 1000mL. Autoclave at 121°C for 20 minutes. The data on the impact of different sucrose concentrations on the survival of aphids are obtained in the following table
[0040]
[0041] (2) The mensuration of toxin transmission rate: the same as the method of measuring toxin transmission rate in embodiment 1, measure the toxin transmission rate of the apterous adults raised in the present embodiment to be 83.3%, the toxin transmission rate is the same as that utilizing nontoxic citrus seedlings. Or the virus transmission rate of ...
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