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Rearing method of non-toxic brown citrus aphids for researching virus transmission molecular mechanism

A molecular mechanism, brown technology, applied in the field of animal husbandry, etc., can solve the problems of differences and little progress in the molecular mechanism of aphid transmission, and achieve the effect of simple production, no impact on the efficiency of virus transmission, and easy preparation

Inactive Publication Date: 2012-07-18
CITRUS RES INST OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sugar is the main source of energy in organisms. It has dual functions of stimulating feeding and nutrition for aphids. Different aphids require different sucrose concentrations. At present, artificial succession has been achieved for many aphid species such as peach aphid and pea aphid. Generation breeding, there is a report on the rearing of the orange aphid for one generation in artificial breeding, but there is no report on the artificial breeding of the brown orange aphid, and the molecular mechanism of the aphid transmission of the citrus decay virus has not made much progress

Method used

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  • Rearing method of non-toxic brown citrus aphids for researching virus transmission molecular mechanism
  • Rearing method of non-toxic brown citrus aphids for researching virus transmission molecular mechanism
  • Rearing method of non-toxic brown citrus aphids for researching virus transmission molecular mechanism

Examples

Experimental program
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Effect test

Embodiment 1

[0019] (1) subculture of non-toxic brown orange aphid on citrus seedlings

[0020] Citrus aphids are collected from the field, and a non-toxic population is established with newly hatched nymphs (nymphs refer to newly born aphids, and newly hatched nymphs refer to newly born aphids that have not touched the leaf surface), and detoxified orange seedlings or seedlings Raising brown citrus aphids, in order to ensure that there are always tender aphids, pruning 20 non-toxic citrus seedlings every week in a greenhouse with a temperature of 25±2°C, and applying 0.5% urea or compound fertilizer to promote hair tips after pruning. Faxin later moved to the insect culture room. The temperature in the insect culture room was set at 25±2°C, and the light:dark ratio was 14:10h. Gently place more than 30 wingless adults on the new shoots of non-toxic citrus seedlings to produce nymphs. After 1-2 days, transfer the adults to another non-toxic seedling. The next generation of 1-2 day-old wing...

Embodiment 2

[0038] The other steps of this embodiment are exactly the same as in Embodiment 1, the difference has the following two points:

[0039] (1) It is the preparation of feeding solution: NaCl 8g, KCl 0.1g, NaCl 2 HPO 4 12H 2 O 3g, KH 2 PO 4 0.1g, 50g of yeast extract powder, adjust the pH to 7.0, make 0, 10%, 20%, 25%, 30%, 35%, 40%, 50% and 60% different sucrose concentration feeding solutions, use deionized Prepare with water, and set the volume to 1000mL. Autoclave at 121°C for 20 minutes. The data on the impact of different sucrose concentrations on the survival of aphids are obtained in the following table

[0040]

[0041] (2) The mensuration of the toxin transmission rate: the same as the method of measuring the toxin transmission rate in Example 1, measure the toxin transmission rate of the wingless adults raised in the present embodiment to be 83.3%, and the toxin transmission rate is the same as that using nontoxic citrus seedlings. Or the virus transmission ...

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Abstract

The invention discloses a rearing method of non-toxic brown citrus aphids for researching a virus transmission molecular mechanism. The method comprises the following steps of: performing continuous rearing by utilizing non-toxic citrus seedlings to obtain the non-toxic wingless brown citrus aphids, preparing a rearing solution, sterilizing, and preparing and rearing in a rearing room. According to the method, the formation of the winged adult aphids is effectively inhibited, the obtainment of the annual non-toxic and wingless adult aphids is realized and the standardization of experimental test insects is ensured by adjusting the number of the wingless adult aphids and next-generation nymphs on non-toxic citrus seedlings. The rearing solution used in the method disclosed by the invention is simple and easy to prepare, the short-term normal survival of the wingless adult aphids after deviating from host plants is realized, the virus transmission efficiency is not affected, and the method is suitable for the requirements for researching the aphid transmission citrus tristeza virus molecular mechanism.

Description

technical field [0001] The invention belongs to the field of insect breeding, and in particular relates to a method for raising non-toxic brown orange aphids for research on the molecular mechanism of toxin transmission. Background technique [0002] The brown orange aphid (Toxoptera citricida, Kirkaldy), also known as the oriental orange aphid, belongs to the family Aphidoidea of ​​the order Homoptera, and is a worldwide distribution of citrus pests. An effective carrier of Citrus tristeza virus (CTV). Citrus tristeza virus (CTV) belongs to the genus Closterovirus and is the largest virus in the known plant virus genome. Citrus decay disease caused by citrus decay virus is an economically important disease that is widely distributed in citrus producing areas in the world and poses a serious threat to the citrus industry worldwide. [0003] The research on artificial diet for aphids began in the 1930s, and the emergence of artificial feeding technology for aphids allowed a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
Inventor 刘金香周常勇李中安宋震李玲娣王永江
Owner CITRUS RES INST OF CHINESE ACAD OF AGRI SCI
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