Oriental lily seed ball virus removing method

Abstract

The invention provides an oriental lily seed ball virus removing method, which specifically comprises the following steps of: A) stripping of explant growth points, B) heat treatment virus removal, C) cleaning and sterilization of explants, D) acquisition, induction and culture of growth points, E) virus-free culture and F) virus detection. The oriental lily seed ball virus removing method has the advantages that the defects existing in the prior art are overcome, the defects caused by a reason that a single method is used are overcome by comprehensively using a heat treatment virus removing method, a shoot tip culture virus removing method and a callus virus removing method, the probability of virus cross infection can be effectively reduced, the virus removal rate is high, the survival rate is high and the culture period of seed balls can be effectively shortened.

Description

technical field [0001] The invention belongs to a method for testing or treating seeds before sowing or planting, in particular to a method for detoxifying lily bulbs. Background technique [0002] Oriental lily is the largest and most beautiful category of lily hybrids. It can emit a pleasant fragrance, so it is also called perfume lily. It is deeply loved by people and is a large category of lily widely cultivated in the world. hybrid line. Lilies mainly reproduce by cuttings and balls, but long-term asexual reproduction causes viruses to accumulate in the body, causing various viral diseases, and seriously affecting the quality of bulbs and cut flowers. In recent years, with the number of lily introductions in my country and With the rapid increase of the planting area and the irregular self-propagation of the bulbs, viral diseases began to be prevalent in various lily planting areas in my country. The general incidence of viral diseases is 40-50%, and the second-generati...

AI Technical Summary

Problems solved by technology

Lilies mainly reproduce by cuttings and balls, but long-term asexual reproduction causes viruses to accumulate in the body, causing various viral diseases, and seriously affecting the quality of bulbs and cut flowers. In recent years, with the number of lily introductions in my country and With the rapid increase of the planting area and the irregular self-propagation of the bulbs, viral diseases began to be prevalent in various lily planting areas in my country. The general incidence of viral diseases is 40-50%, and the second-generation bulbs have a poisonous rate of 90%. The above seriously restricts the improvement of quality and efficiency of my country's lily industry.

[0003]At present, the detoxification treatment of lily at home and abroad mainly adopts heat treatment detoxification alone, shoot tip culture detoxification and callus detoxification and the combination of each other. When these methods are used alone, the efficiency is low, and the detoxification effect is poor. It is generally better to use the shoot tip culture detoxification method. The conventional steps are: cut the 0.2~0.3mm shoot tip through the top meristem of the plant, induce callus, and then differentiate from the callus to produce bulb buds, which grow into small plants to obtain virus-free seedlings

For example, the Chinese patent No. 200410079513.2 discloses a "method for in-bottle production of oriental lily detoxification small seed balls", which adopts stem tips as explants, undergoes sterilization and induces culture to obtain detoxification oriental lily bulbs The problem that this detoxification tissue culture method exists is: cut very little shoot apical meristem, cultivate on the MS basic medium that adds 6-BA 1.0~2.0 mg / L and NAA 0.2~0.5mg / L In 20-25 days, callus and adventitious buds will be formed at the shoot tip at the same time, but the adventitious buds differentiated from the callus are prone to genetic variation; and the virus has not been further detected and monitored, so it is difficult to understand the virus-free situation