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Manufacturing method of ceramide production accelerating agent

A ceramide, a technology for a manufacturing method, applied in the directions of microorganism-based methods, biochemical equipment and methods, preparations for skin care, etc., to achieve the effect of improving moisturizing ability

Inactive Publication Date: 2012-07-11
KAO CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] However, there has been no report on the preparation of ceramide production accelerators or glucosylceramide production accelerators using microorganisms of the genus Leuconostoc.

Method used

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  • Manufacturing method of ceramide production accelerating agent

Examples

Experimental program
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Effect test

Embodiment 1

[0046]

[0047] First, the culture medium is prepared according to the following method: Soak soybeans in water for 12 hours to allow the soybeans to fully absorb water, then grind and crush the absorbed soybeans, filter them to remove liquid components, and drain the remaining residues to obtain bean dregs. The okara was mixed with glucose and water, and the concentrations of the okara and glucose in the mixture were respectively 10w / v% and 1w / v%, and the mixture was used as a culture medium.

[0048] Then, add 100ml of the above-mentioned culture medium to the Erlenmeyer flask, inoculate a certain amount of Leuconostoc mesenteroides (preserved in CICIM, Industrial Microbiology Resource and Information Center, Jiangnan University, No. CICIM B1254), at 30°C Cultured for 3 days.

[0049] The resulting culture was then centrifuged (3000 rpm, 10 minutes). The remaining supernatant was diluted with 2.5 times of absolute ethanol, and 1ml of the mixture was taken as sample A of t...

Embodiment 2

[0068] In the preparation process of the ceramide / glucosylceramide production accelerator of Example 1, as a microorganism of the genus Leuconostoc, Leuconostoc mesenteroides (preserved in Jiangnan University Industrial Microorganism Resources and Information Center CICIM, No. CICIM B1364), except that it was cultured in the same manner as in Example 1.

[0069] The resulting culture was then centrifuged (3000 rpm, 10 minutes). The remaining supernatant was diluted with 2.5 times absolute ethanol, and 1ml of the mixture was taken as sample D of the ceramide / glucosylceramide production accelerator of the present invention, and stored in a refrigerator at 4°C for use as an additive for cell culture.

[0070]The above-mentioned mixed solution diluted with absolute ethanol was ultrasonically treated for 10 minutes, centrifuged (3000rpm) for 10 minutes, and the supernatant (about 70ml) was collected. The supernatant was filtered with filter paper to obtain the filtrate, and the fil...

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Abstract

The invention provides a manufacturing method of a ceramide production accelerating agent and / or a glucosylceramide ceramide production accelerating agent. The method comprises the following steps of: culturing a microorganism of Leuconstoc in a culture medium containing bean dregs and saccharides, and obtaining the ceramide production accelerating agent and / or the glucosylceramide ceramide production accelerating agent from supernate. According to the manufacturing method provided by the invention, the ceramide production accelerating agent and / or the glucosylceramide ceramide production accelerating agent can be efficiently obtained at low cost through utilizing an easily obtained substance as the culture medium and utilizing a specific microorganism.

Description

technical field [0001] The present invention relates to a method for producing a ceramide production promoter and / or a glucosylceramide production promoter capable of promoting the production of ceramide and / or glucosylceramide in the skin. Background technique [0002] The skin has the ability to produce ceramide and glucosylceramide. In the skin, especially in the stratum corneum of the epidermis, ceramide synthesized and secreted by epidermal cells is accumulated, which is the main component of intercellular lipids. The ceramide synthesized by epidermal cells is temporarily stored in the form of glucosylceramide or sphingomyelin; it is then excreted outside the cell, where glucocerebrosidase (glucocerebrosidase) and sphingomyelinase (sphingomyelinase) ) to form ceramide again, thereby functioning as an intercellular lipid. Ceramides and glucosylceramides in the skin have physiological effects of improving the moisturizing function of the skin, the barrier function of th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/04A61K8/99A61Q19/00C12R1/01
Inventor 孔凡旗
Owner KAO CORP
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