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Application of Paenibacillus macerans strain in dissolution of phosphorus source in soil or eutrophic water

A bacillus and eutrophication technology, applied in the field of microorganisms, to achieve the effects of preventing and controlling eutrophication, promoting growth, and increasing the content of available phosphorus

Inactive Publication Date: 2012-07-11
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Indeed, P. macerans has been found to be involved in plant growth promotion (Timmusk et al. 1999) and pathogen suppression (Bae et al. 2000; Timmusk 2003; Li et al. 2011a,b), but not yet Report on Paenibacillus macerans as Phosphorus-Solubilizing Bacteria

Method used

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  • Application of Paenibacillus macerans strain in dissolution of phosphorus source in soil or eutrophic water
  • Application of Paenibacillus macerans strain in dissolution of phosphorus source in soil or eutrophic water
  • Application of Paenibacillus macerans strain in dissolution of phosphorus source in soil or eutrophic water

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 establishes standard phosphorus curve

[0039] Will KH 2 PO 4 Dry at 105°C for 4 hours, accurately weigh 0.2195g KH 2 PO 4 Dissolve in water and transfer to a 1000ml volumetric flask, add 400ml of water and 5ml of concentrated sulfuric acid, and set the volume to 1000ml to obtain a 50mg / l phosphoric acid standard solution. Use 50mg / l phosphoric acid standard solution as the phosphorus source, prepare standard solutions with a series of concentrations, and establish a line about OD 450 Value and the standard curve of phosphorus content in the solution, through the standard curve can be measured by the OD in the experiment 450 value to get the corresponding phosphorus content.

[0040] Wherein, the determination of phosphorus content adopts phosphomolybdenum yellow photometric method, specifically:

[0041] (1) Weigh 2.4g NaOH, add water and keep stirring, and make the volume to 100ml to make 6N NaOH solution;

[0042] Weigh 0.25g of 2,4-dinitrophenol ...

Embodiment 2

[0045] Embodiment 2 prepares seed liquid

[0046] Streak the preserved Paenibacillus macerans ZJU0902 on the NA agar plate with the three-section line method, and culture it at 28°C for 24 hours; put a single colony of Paenibacillus macerans ZJU0902 into the LB liquid medium, and incubate at 28°C. ℃, 160r / min shaking in a shaker for 12h, to make a seed liquid, the concentration of bacteria in the seed liquid is 10 8 CFU / ml.

Embodiment 3

[0047] The mensuration of phosphorus dissolving ability in embodiment 3 solid medium

[0048] In NBRIP liquid medium (glucose 10g, magnesium chloride hexahydrate 5g, magnesium sulfate heptahydrate 0.25g, potassium chloride 0.2g, ammonium sulfate 0.1g, distilled water 1000ml, pH 7.0), add 5g / l inorganic phosphorus Ca respectively 3 (PO 4 ) 2 , CaHPO 4 、FePO 4 and AlPO 4 And 5ml / l organophosphate phytic acid as phosphorus source, then add agar (20g / l) to prepare solid medium. Invert the plate after autoclaving, dip the Paenibacillus macerans ZJU0902 purified and cultured in Example 2 onto the plate with a sterilized toothpick, and culture at 30° C. in the dark. After 4 weeks, the diameter of the transparent phosphorus dissolution circle was detected. Repeat 4 times.

[0049] The results showed that Paenibacillus macerans ZJU0902 could dissolve Ca 3 (PO 4 ) 2 , CaHPO 4 and phosphorus in phytic acid, and the phosphorus solubility of phytic acid is the largest. In solid...

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Abstract

The invention discloses an application of a Paenibacillus macerans strain in the dissolution of phosphorus sources in soil or eutrophic water. The Paenibacillus macerans strain is Paenibacillus macerans ZJU0902 with a preservation number of CCTCC NO:M209158; the phosphorus source is at least one of inorganic phosphorus sources and organic phosphorus sources. According to the invention, Paenibacillus macerans ZJU0902 is used for the dissolution of inorganic and organic phosphorus sources in soil, and has the characteristics of stability, high efficiency, and wide spectra for phosphorus dissolution; After a microbial fertilizer prepared by mixing the bacterial strain fermentation broth with a fertilizer matrix is applied to soil, dominant microflora are formed rapidly; the fertilizer efficiency is good; the effective phosphorus content of the soil can be improved significantly; and the healthy growth of plants is promoted; the bacterial strain is used for the dissolution of inorganic and organic phosphorus sources in eutrophic water, can promote the absorption of superfluous phosphorus element in water by water plants, and thus prevent and control water eutrophication.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to the application of a Paenibacillus macerans strain as a phosphorus source in dissolving soil or eutrophic water bodies. Background technique [0002] Plant growth requires some essential nutrients, and phosphorus is one of the main elements that can affect the healthy growth of plants. Phosphorus is not only a component of many important compounds in plants, but also participates in important metabolisms in plants in various ways. However, 90% of the phosphorus in the soil exists in an insoluble state, which may be due to the fact that the phosphorus in the natural soil easily interacts with Fe in acidic conditions. 3+ 、Al 3+ Plasma complexation to form FePO 4 and AlPO 4 and other insoluble phosphates; under alkaline conditions and with Ca 2+ Ions complex to form Ca 3 (PO 4 ) 2 or CaHPO 4 Phosphorus, such as inert phosphates, cannot be directly absorbed by plants,...

Claims

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Application Information

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IPC IPC(8): C05F11/08C05G3/00
Inventor 李斌周青石雨唐乔梅邱慧谢关林
Owner ZHEJIANG UNIV
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