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Method of tissue culture propagation for rhizome polygonati

A tissue culture and Polygonatum chinensis technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as uneconomical, inconvenient planting management and promotion, long seedling raising time, etc., to shorten production time and cost, and improve reproduction. Speed, damage reduction effect

Inactive Publication Date: 2012-07-11
重庆市秀山红星中药材开发有限公司
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Because sealwort seeds are difficult to collect, the germination rate of seeds is low, and the seed propagation takes a long time to raise seedlings, which greatly increases the cultivation cost of sealwort, which is not conducive to the artificial large-scale planting of sealwort
Therefore, in the current artificial cultivation, propagation mainly relies on rhizome propagation, but this method has a low propagation coefficient and a large amount of seed rhizomes, which is not economical, but also limits the yield potential of Rhizoma Polygonatum, which is inconvenient for planting management and promotion, so the expansion of planting area is restrained

Method used

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Embodiment Construction

[0022] The present invention will be further described below in conjunction with embodiment.

[0023] First prepare the following materials.

[0024] Basic medium: MS+30g / L sucrose+5g / L agar powder, pH value is 5.8-6.0.

[0025] Artificial climate incubator: control the start and end time of the light cycle from 8:00 to 20:00, the daily temperature is 25°C, the night temperature is 18°C, the humidity is 60%, and the light intensity is 2000-2400lx.

[0026] Induction medium: MS+6-BA2.0mg / L+2,4-D1.0mg / L.

[0027] Subculture medium: MS+6-BA2.0mg / L+2,4-D2.0mg / L.

[0028] Differentiation medium: MS+6-BA3.0mg / L+IAA1.0mg / L.

[0029] Strong seedling medium: MS+6-BA2.0mg / L+IAA0.5mg / L.

[0030] Rooting medium: MS+IAA0.5mg / L.

[0031] 100 sections of 0.7cm Polygonatum Rhizome.

[0032] Then start the tissue culture work.

[0033] Take the Rhizome of Rhizoma Polygonatum with Buds, cut it into small pieces of 0.5-1.0cm with a knife, soak it in the aqueous solution of detergent for 5...

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Abstract

The invention provides a method of tissue culture propagation for rhizome polygonati, comprising the steps of introducing and sterilizing, tissue inducing, subculturing, and carrying out differentiation culture, strong seedling culture and rooting culture. The beneficial effects of the method are that seedlings can be produced in large amount by the tissue culture, and only seven months are needed from tuber to rooted seedling by culturing; thus production time and cost of seedlings are greatly decreased and propagation speed is increased.

Description

technical field [0001] The invention relates to the technical field of cultivation of Chinese medicinal materials, in particular to a method for tissue culture and propagation of Rhizoma Polygonatum. Background technique [0002] Polygonatum is a perennial herb of the genus Polygonatum in the Liliaceae. According to the classic records of traditional medicine, Rhizoma Polygonatum belongs to the precious Chinese medicine, which has the effects of widening the middle and replenishing Qi, nourishing the kidney and replenishing essence, nourishing yin and moistening the lung, and promoting body fluid and nourishing the spleen. Indications dryness of the lungs and dry cough, physical weakness and fatigue, palpitations, shortness of breath, chronic illness, dry mouth, diabetes, high blood pressure and other diseases. It has good effects on the treatment of cardiovascular diseases, tuberculosis, chronic hepatitis, antibacterial, detoxification, anti-fatigue, anti-aging, lowering b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 赵勇彪祝利杨再江向廷军何全慧
Owner 重庆市秀山红星中药材开发有限公司
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