Clostridium beijerinckii strain and screening method and use thereof

A technology of Clostridium beijerinckii and bacteria strains, applied in the field of bioengineering, can solve the problem of less research on Clostridium beijerinckii, and achieve the effect of reducing costs

Inactive Publication Date: 2012-07-04
GUANGXI ACAD OF SCI
View PDF3 Cites 15 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Domestic research mainly focuses on the transformation of Clostridium acetobutylicum, and less research on Clostridium bejejerinckii

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: Clostridium beijerinckii ( Clostridium beijerinckii ) Isolation, screening and identification of gxas.28

[0023] Step 1: Sampling

[0024] Samples were collected from Nanning, Guangxi Zhuang Autonomous Region.

[0025] Step Two: Enrichment

[0026] Enrichment medium components: molasses 100 g (the sugar content of molasses is about 50%, provided by Guangxi Fenghao Sugar Industry Co., Ltd.), yeast powder 5 g, calcium carbonate 3.0 g, superphosphate 0.7 g, ammonium sulfate 3.0 g , and dilute to 1000 mL with distilled water, pH 6.2-6.5. Mix the above ingredients evenly, divide into 30 mL test tubes, seal with rubber stoppers, and autoclave at 115°C for 20 min.

[0027] 1 g of each sample was weighed and placed in test tubes containing molasses fermentation medium, heat-shocked at 80°C for 10 minutes to kill heat-labile bacteria and vegetative cells, and then placed in a 37°C incubator for static culture for 72 h, Select the fermented mash with bu...

Embodiment 2

[0045] Embodiment 2: Clostridium beijerinckii ( Clostridium beijerinckii ) gxas.28 fermented to produce butanol

[0046] Plate medium (TYA medium): 40 g glucose, 2 g beef extract, 2 g yeast powder, 6 g peptone, 3 g ammonium acetate, 0.5 g potassium dihydrogen phosphate, 0.2 g magnesium sulfate heptahydrate, sulfurous acid heptahydrate Iron 0.01 g, agar 20 g, distilled water 1000 mL, pH 6.5, autoclaved at 121 °C for 20 min.

[0047] Seed medium: glucose 40 g, beef extract 2 g, yeast powder 2 g, peptone 6 g, ammonium acetate 3 g, potassium dihydrogen phosphate 0.5 g, magnesium sulfate heptahydrate 0.2 g, ferrous sulfate heptahydrate 0.01 g, distilled water 1000 mL, pH 6.5, autoclave at 121°C for 20 min.

[0048] Molasses fermentation medium: molasses 100 g, yeast powder 5 g, calcium carbonate 3.0 g, superphosphate 0.7 g, ammonium sulfate 3.0 g, dilute to 1000 mL with distilled water, pH 6.2-6.5. Mix the above ingredients evenly, divide into 30 mL test tubes, seal with rubb...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses high-butanol-yield clostridium beijerinckii, which is obtained by sampling, enriching, primary screening, separating and secondary screening. The strain is identified as clostridium beijerinckii which belongs to the class of clostridium beijerinckii gxas.28. The collection number of the strain is CCTCCM2011454. According to the invention, butanol is produced by using molasses as a raw material and by fermentation of clostridium beijerinckii. The butanol yield is 9.42 to 13.11g/L, and the ratio of the butanol to total solvent reaches 70 to 85 percent.

Description

technical field [0001] The invention relates to microbial screening, cultivation and application thereof, in particular to the application of Clostridium beijerinckii to ferment and produce butanol, and belongs to the technical field of bioengineering. Background technique [0002] As an excellent organic chemical raw material, butanol is mainly used in the fields of solvents, antioxidants, plasticizers, synthetic drugs and fine chemicals. With the diversified development of biomass energy, butanol is considered to be one of the most potential new liquid fuels because of its high energy density, low hydrophilicity, low vapor pressure, and high octane number. With the depletion of petroleum resources and the rising price of crude oil, countries around the world are increasingly concerned about energy security and resource security, so the production of butanol by microbial fermentation is favored by people. [0003] The fermentation of bio-butanol used to be the second large...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C12Q1/04C12P7/16C12R1/145
Inventor 裴建新黄日波庞浩左文朴陈东黎贞崇黄志民
Owner GUANGXI ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap