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Method for preparing H2O2 biosensor of MWNTs-IL/Cyt c/GCE

A biosensor, H2O2 technology, applied in the field of preparation of H2O2 biosensors, can solve the problems of poor fixation and high detection line of biomolecules, and achieve the effects of simple detection process, low detection line, and increased adsorption and catalytic activity

Inactive Publication Date: 2012-06-20
NORTHWEST NORMAL UNIVERSITY
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Problems solved by technology

More interestingly, more and more attention has been focused on polymer-enzyme sensors with poor enzyme immobilization and relatively high detection lines for biomolecules.

Method used

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  • Method for preparing H2O2 biosensor of MWNTs-IL/Cyt c/GCE
  • Method for preparing H2O2 biosensor of MWNTs-IL/Cyt c/GCE
  • Method for preparing H2O2 biosensor of MWNTs-IL/Cyt c/GCE

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Embodiment Construction

[0019] The water used in the experiment process was double distilled water, and the reagents used in the experiment were all analytically pure. The carboxylation of multi-walled carbon nanotubes was performed by ultrasonication for 8 hours with concentrated hydrochloric acid and hydrogen peroxide in a volume ratio of 3:1, and then washed to neutral. Durability, dry at 70°C before use.

[0020] Apparatus and reagents used in this embodiment

[0021] CHI 660c electrochemical workstation (Shanghai Chenhua Instrument Co., Ltd.) was used for alternating cyclic voltammetry experiments, and a quartz tube heating automatic double pure water still (1810B, Shanghai Asia Pacific Technology Glass Co., Ltd.) was used for distilling double distilled water. Electronic balance (Beijing Sartorius Instrument Co., Ltd.), used for weighing medicines. Ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.). Aluminum oxide grinding powder (0.30 μm, 0.05 μm, Shanghai Chenhua Instrument Reagen...

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Abstract

The invention provides a method for preparing H2O2 biosensor of MWNTs-IL / Cyt c / GCE, which includes the following steps: performing chemical reaction between a multi-wall carbon nano tube subjected to carboxylation and ionic liquid subjected to amidation, so as to form a carbon nano tube subjected to amidation; and then fixing a cytochrome c on a glassy carbon electrode modified by the carbon nanotube subjected to amidation to increase the adsorption and catalytic activity of the cytochrome c. Through adopting the method, the detection line of the hydrogen peroxide obtained from the ampere response can reach 1.3*10<-8>M, and the MWNTs-IL / Cyt c / GCE has sensitive response to the oxidation of the hydrogen peroxide.

Description

technical field [0001] The present invention relates to a kind of H of MWNTs-IL / Cyt c / GCE 2 o 2 Preparation methods of biosensors. Background technique [0002] In recent years, the research and application of adsorption and immobilization of biomolecules (such as proteins and enzymes) on inorganic materials has attracted attention. Especially compared with industrial inorganic catalysts, the specificity of enzymes and the effectiveness of the reaction make enzymes better biocatalysts. [0003] Since the discovery of carbon nanotubes, due to their good biocompatibility, they combine with biological systems to form functional hydrogen bonds, and have been well applied in bio-nanoelectrochemistry. In addition, functionalized carbon nanotube-modified electrodes have stable electrocatalytic responses to many important biomolecules involved in human health. More interestingly, more and more people have focused on polymer-enzyme sensors, which have relatively poor immobilizati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
Inventor 刘秀辉卜彩红张义军南志汉裘宇卢小泉
Owner NORTHWEST NORMAL UNIVERSITY
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