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Rapid detection kit of nitrite nitrogen in aquiculture water and detection method using same

A technology of water nitrite and detection kit, which is applied in the direction of material analysis by observing the impact on chemical indicators, and analysis by making materials undergo chemical reactions, can solve problems affecting breeding benefits and fish poisoning, etc. Achieve broad application prospects, easy storage, and reduce testing costs

Inactive Publication Date: 2012-06-20
TIANJIN SHENGJI GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In the process of aquatic animal breeding, especially in high-density farming mode, nitrite is one of the main factors restricting the normal growth of aquatic animals. When its concentration reaches a certain value, it will not only cause direct poisoning to fish, but also induce various diseases, thus affecting the breeding benefits

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A rapid detection kit for nitrite nitrogen in water for aquaculture is characterized in that the kit is equipped with:

[0026] 10ml stoppered cuvette: 1;

[0027] Color comparison card: 1; (use the existing color comparison card)

[0028] Straw: 1;

[0029] 20ml dropper bottles: 2;

[0030] Reagent A: 20ml;

[0031] Reagent B: 20ml;

[0032] Preparation of reagent A (500ml): Measure 50ml of phosphoric acid in a 200ml beaker, the concentration of phosphoric acid is 15mol / L=1.70g / mL, add 200ml of deionized water to mix evenly; weigh 20g of 4-aminobenzenesulfonamide into In the beaker, after making it dissolve, transfer it to a 500ml volumetric flask, set the volume to 500ml with deionized water, subpackage, 20ml / bottle, and store in the dark;

[0033] Preparation of Reagent B (500ml): Weigh 1g of 1-naphthylethylenediamine dihydrochloride and dissolve it in 300ml of deionized water, transfer it to a 500ml volumetric flask, dilute it to 500ml with deionized water, pac...

example 1

[0040] Nitrite nitrogen rapid detection kit precision test, the specific operation is:

[0041] The prepared nitrite nitrogen rapid detection kit was tested for repeatability, intermediate precision and reproducibility of the test samples respectively. Repeatability test: within the specified range, design 8 solutions with different nitrite nitrogen contents (measured by spectrophotometer), make 3 parallels for each concentration, measure 3 parallels for each concentration 3 times, and use the obtained results to determine The results were evaluated; intermediate precision test: in this laboratory, 3 different analysts used different equipment to prepare the solutions of the above 8 different concentrations, and each concentration made 3 parallels, and the 3 parallels of each concentration were all tested. Measured 3 times, evaluated with the obtained measurement results; and evaluated with the obtained measurement results and the measurement results obtained by the repeatabil...

example 2

[0043] Nitrite nitrogen rapid detection kit shelf life test:

[0044] Long-term test: put the three batches of prepared kits at 4°C and store them in the dark, and test the same batch of water samples at 0, 3, 6, 9, and 12 months respectively. The results show that nitrite nitrogen The actual measured value of the content is consistent with the measured value of the spectrophotometer.

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PUM

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Abstract

The invention relates to a rapid detection kit of nitrite nitrogen in aquiculture water. The kit internally comprises one 10 mL stoppered cuvette, one colorimetric card, one suction pipe, two 20 mL dropping bottles, 20 mL of reagent A and 20 mL of reagent B. The detection method using the kit comprises the following specific operation steps of: (1) washing the cuvette 2-3 times with the water to be detected; (2) sampling 5 mL of water sample, adding 5 drops of the reagent A, then adding 5 drops of reagent B and uniformly shaking; and (3) covering the cuvette, lightly shaking, standing for 1-3 minutes, closing the cuvette to the blank of the colorimetric card, and comparing the color of the liquid in the cuvette with the color of the colorimetric card to obtain the content (mg / L) of nitrite nitrogen in the detected water sample. According to the invention, the content of nitrite nitrogen in the water sample to be detected is displayed by a developing agent, thus the rapid detection kit has the characteristics of being accurate, rapid, convenient, inexpensive and the like, does not need for any equipment, and adopts a counting drop method to measure; and is used for field rapid detection of water quality of aquiculture water, domestic sewage, industrial wastewater and the like, the rapid detection kit is very simple in use method.

Description

technical field [0001] The invention relates to a chemical composition detection kit and a detection method thereof, in particular to a rapid detection kit of nitrite nitrogen in aquaculture water and a detection method thereof. Background technique [0002] In the process of aquatic animal breeding, especially in high-density farming mode, nitrite is one of the main factors restricting the normal growth of aquatic animals. When its concentration reaches a certain value, it will not only cause direct poisoning to fish, but also induce various diseases, thereby affecting the breeding benefits. [0003] After nitrite poisoning, the oxygen-carrying capacity of the blood is weakened, and the breeding objects are more likely to develop symptoms similar to hypoxia. When chronic nitrite poisoning occurs to aquatic animals, the symptoms are not obvious, and it is difficult to see with the naked eye, which seriously affects the growth of aquatic products; if the poisoning is deep, t...

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 伦艳霞
Owner TIANJIN SHENGJI GRP CO LTD
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