Device and method for evaluating malignancy degree of neuroblastoma

Abstract

The invention provides a device for evaluating the malignancy degree of neuroblastoma. The device is characterized by comprising a glass microprobe filled with electrolyte, a Ag / AgCl electrode placed in the glass microprobe and dipped in the electrolyte, a reference Ag / AgCl electrode, a culture vessel containing cells and a cell culture solution, an external current amplifier, a digital-to-analog / analog-to-digital converter, an HPICM (hopping probe ion conductance microscopy) scanning control system, an HPICM scanning probe and a computer control system. According to the method for evaluating the malignancy degree of the neuroblastoma, through the hopping probe ion conductance microscopy technology, the malignancy degree of the neuroblastoma is evaluated by identifying relative ratios of different cell subsets in the neuroblastoma according to morphological features, such as heights and volumes of the different cell subsets in the neuroblastoma. According to the device and the method provided by the invention, the cells can be detected in real time under a normal physiological condition; the whole diagnostic process is obvious and simple; samples are not needed to be subjected to special treatment; and the diagnostic accuracy is high.

Description

(1) Technical field: [0001] The present invention provides a device and method for evaluating the malignancy of neuroblastoma, especially a method for evaluating the malignancy of neuroblastoma by identifying the relative ratios of different cell subtypes, more specifically, using detection Needle-hopping mode scanning ion conductance microscopy is a method for diagnosing the malignancy of neuroblastoma by identifying the relative ratio of different subtypes based on the morphology of different cell subtypes in neuroblastoma. (two) background technology: [0002] Neuroblastoma (NB) is a common extracranial malignant solid tumor in young children. In the past 20 years, there has been no suitable diagnosis and treatment for it, especially when the disease is not diagnosed early and develops to the third and fourth stages, the cure rate and prognosis are even worse. The main difficulty in the diagnosis and treatment of neuroblastoma stems from the heterogeneity of the tumor ce...

AI Technical Summary

Problems solved by technology

However, due to the limitation of the optical diffraction limit, the highest resolution of ordinary optical microscopes cannot meet the needs of distinguishing type I and type N cells with similar shapes; biological samples need to be solidified and gold-sprayed in electron microscope detection, which is not only time-consuming and laborious but also It will change or even destroy the surface morphology of the sample and reduce the accuracy of identification; due to the negative feedback control of the force between the needle tip and the sample, the slight contact between the atomic force microscope (AFM) probe and the living cell body is inevitable, and at the scanning height For complex biological samples with rapid changes and low attachment to the substrate (for example: round neuroblastoma type N and type I cells), there are insurmountable difficulties in negative feedback control microscopy

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