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Mouse cataract model and construction method thereof

A construction method, cataract technology, applied in the fields of biotechnology and medicine, which can solve the problems of unknown mutated genes and locations

Inactive Publication Date: 2012-05-16
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After further backcrossing with BALB / cAnSlac for 10 generations, pure-line mice with cataract symptoms were bred, and the stable autosomal dominant genetic characteristics were maintained, but the specific mutated genes and positions were unknown

Method used

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  • Mouse cataract model and construction method thereof
  • Mouse cataract model and construction method thereof
  • Mouse cataract model and construction method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: Construction of mouse cataract pure line model

[0040] 1. Backcross

[0041] (a) BALB / cAnSlac mice were crossed with Slac:ICR mice to obtain offspring, and cataract mice were screened out;

[0042] (b) Cataract mice were used as the male parent and the BALB / cAnSlac strain was used as the female parent to obtain the F1 generation; cataract-symptomatic mice were selected for the F1 generation to be backcrossed with BALB / cAnSlac for 10 generations, and bred into pure-line mice with cataract symptoms BALB / c+ / +;

[0043] (c) BALB / c+ / + mice were continuously backcrossed with C3H / He for 2 generations to obtain the F2 generation, and cataract-symptomatic mice were selected for genetic identification.

[0044] 2. Identification of backcrosses

[0045] The sequence of selected STR sites was obtained from NCBI (National Center for Biotechnology Information), and then primers were designed for these sites, and the main primer design was mainly completed by the Oligo6...

Embodiment 2

[0057] Example 2: Genetic Identification of Cataract Spontaneous Mutation Genes

[0058] (1) Fine positioning

[0059] The genetic markers used for fine mapping were further selected from NCBI (National Center for Biotechnology Information) to obtain the sequence of STR loci, and then primers were designed for these loci, and the main primer design was mainly completed by the Oligo6.0 program. Through genome-wide scanning and haplotype analysis, the cataract-related genes were fine-mapped within about 11cM between D1Mit236 and D1Mit19 on chromosome 1.

[0060] Table 4 The STR site selected by fine positioning in the present invention

[0061]

[0062] Table 5 Whole genome scanning and fine mapping results

[0063] STR

Genetic position (cM)

number of homozygotes

number of heterosons

The proportion of homozygotes %

chain

D1MIT410

17

16

67

19.3

yes

D1MIT236

25.7

6

76

7.3

yes ...

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Abstract

The invention belongs to the fields of biotechnologies and medicine, and in particular relates to construction and application of a mouse cataract spontaneous mutant gene pure line BALB / cAnSlac-Crygc-del. By the method, various mutant mice can be screened, and a solid foundation is laid for studying the pathogenesis and treatment method of cataract. The cataract symptom pure line mouse BALB / cAnSlac-Crygc-del obtained by the method can be used for treating and studying congenital cataract and can make prominent contribution to preventing and treating cataract.

Description

technical field [0001] The invention belongs to the fields of biotechnology and medicine, and specifically relates to the construction and application of a mouse cataract spontaneous mutation gene pure line BALB / cAnSlac-Crygc-del. Background technique [0002] Mice have become an important model organism for the study of human diseases, and the identification of functional genes related to mammalian development mostly comes from mice with spontaneous or induced mutations. Congenital cataract in mice is the clouding of the lens at birth or within the first year after birth. The phenotype of cataract in mice is mostly caused by single gene mutation, and has a high degree of genetic heterogeneity, which is related to the development and differentiation of the lens Gene mutations can form hereditary cataracts. [0003] In 2006, in the F1 population of the hybrid F1 population of BALB / cAnSlac inbred mice and ICR outbred strains, two mice with spontaneous mutations leading to cat...

Claims

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Application Information

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IPC IPC(8): A01K67/02C12Q1/68
Inventor 鲍世民李凯赵国际陈烨孙宗国
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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