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Antibody chip for adopting in early screening and diagnosis of liver cancer, and kit thereof

An antibody chip and kit technology, applied in the field of biomedicine, can solve the problems of limited sensitivity and specificity, low tumor positive detection rate, etc., and achieve the effect of high accuracy.

Inactive Publication Date: 2012-05-09
ZHONGSHAN HOSPITAL FUDAN UNIV
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AI Technical Summary

Problems solved by technology

[0004] Tumor serum markers have been widely used clinically as auxiliary indicators for the diagnosis of tumors, and they have important reference value in the diagnosis of liver cancer. Only relying on a single indicator for determination has limited sensitivity and specificity, especially for early tumors with a low positive detection rate. In order to make up for the lack of single-indicator detection and improve the accuracy of tumor diagnosis, multi-indicator tumor Combination of markers to detect a tumor

Method used

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  • Antibody chip for adopting in early screening and diagnosis of liver cancer, and kit thereof
  • Antibody chip for adopting in early screening and diagnosis of liver cancer, and kit thereof
  • Antibody chip for adopting in early screening and diagnosis of liver cancer, and kit thereof

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Embodiment

[0027] 1. Antibody chip and its preparation:

[0028] (1) Reagents used:

[0029] Antibody Manufacturer Item No. AFP Monoclonal Antibody R&D MAB13691 HGF Monoclonal Antibody R&D MAB694 IGF Monoclonal Antibody R&D MAB291 IL-6 monoclonal antibody R&D MAB206 IL-8 monoclonal antibody R&D MAB208 IL-10 monoclonal antibody R&D MAB2172 TGFβ1 monoclonal antibody R&D MAB2461 VEGF monoclonal antibody R&D MAB3045 Biotinylated TGFβ1 polyclonal antibody R&D BAM2462

[0030] Prepare PBS buffer solution (pH7.4) containing 30% glycerol as the chip sample solution;

[0031] Ultrathin nitrocellulose membrane PATH TM The protein chip was purchased from GenTel Biosurfaces in the United States;

[0032] (2) Preparation method:

[0033] Spotting: Dilute 8 kinds of monoclonal capture antibody to 0.5 mg / ml with the chip spotting solution, divide the substrate into 14 subarray areas, and prepare the an...

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Abstract

The present invention provides an antibody chip for adopting in early screening and diagnosis of liver cancer, and a kit thereof. The antibody chip for adopting in early screening and diagnosis of the liver cancer comprises a substrate, specific antibodies of cancer-related protein, a positive control and a negative control, wherein the specific antibodies, the positive control and the negative control are loaded on the substrate. The chip is characterized in that: the cancer-related protein comprises HGF, IGF, IL-6, IL-8, IL10, TGFbeta1 and VEGF. The kit for adopting in early screening and diagnosis of the liver cancer is characterized in that: the kit comprises a quantitative antibody chip adopting HGF, IGF, IL-6, IL-8, IL10, TGFbeta1 and VEGF as detection objects, antigen standard materials of HGF, IGF, IL-6, IL-8, IL10, TGFbeta1 and VEGF, and biotinylated detection antibodies of HGF, IGF, IL-6, IL-8, IL10, TGFbeta1 and VEGF. The chip and the kit of the present invention have the high accuracy.

Description

technical field [0001] The invention relates to an antibody chip and a kit used for early screening and diagnosis of liver cancer, belonging to the technical field of biomedicine. Background technique [0002] Hepatocellular carcinoma (HCC) is one of the common malignant tumors. Its onset is insidious, its malignancy is high, and its mortality rate is high. Therefore, early diagnosis is very important to improve the survival rate of patients. At present, alpha-fetoprotein (AFP) combined with imaging and pathological examinations is mainly used for early diagnosis of liver cancer clinically; however, the specificity and sensitivity of AFP for liver cancer screening are not very satisfactory. With the continuous development of molecular biology, a variety of new markers have been discovered one after another. These markers involve several aspects such as protein antigens, enzymes and isoenzymes, cytokines, and related genes of liver cancer, so they have become a hot spot in li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/577
Inventor 康晓楠刘银坤高冬梅
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
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