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Mesoporous silicon dioxide nano particle with bifluorescence mark

A mesoporous silica, nanoparticle technology, applied in the direction of nanotechnology, nanotechnology, nano-optics, etc. for sensing

Inactive Publication Date: 2012-05-09
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, pH fluorescent ratiometric probes based on this dye have not been reported yet.

Method used

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  • Mesoporous silicon dioxide nano particle with bifluorescence mark
  • Mesoporous silicon dioxide nano particle with bifluorescence mark
  • Mesoporous silicon dioxide nano particle with bifluorescence mark

Examples

Experimental program
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Effect test

preparation example Construction

[0033] A mesoporous silica nanoparticle with double fluorescent labels, the preparation method comprising:

[0034] a) Vibrate and mix Rhodamine 6G and (3-aminopropyl)triethoxysilane at room temperature and avoid light until the fluorescence of Rhodamine 6G disappears to obtain R6G-APTS;

[0035] b) Fluorescein isothiocyanate and (3-aminopropyl)triethoxysilane were vibrated and mixed for 2 hours at room temperature in the dark to obtain FITC-APTS;

[0036] c) Mix R6G-APTS and FITC-APTS with ethyl orthosilicate, then add an appropriate amount of deionized water, cetyltrimethylammonium bromide and sodium hydroxide solution, and heat in a water bath at 80°C Vibrate for 2 hours and mix well; then centrifuge the solution to collect the precipitate; then treat the precipitate with ethanol containing 1% HCl and redissolve it by ultrasonication for 5 hours; the redissolved sample is redissolved by centrifugation and ultrasonication, and finally centrifuged Obtained mesoporous silica ...

Embodiment 1

[0038] Example 1: Synthesis of R6G-FITC-MSN

[0039] 1) Synthesis of R6G-APTS:

[0040] Add Rhodamine 6G (0.2 g) to 10 ml APTS[(3-aminopropyl)triethoxysilane] in a capped tube or bottle, shake and mix well at room temperature in the dark until Rhodamine 6G The fluorescence disappears, and R6G-APTS can be prepared;

[0041] 2) Synthesis of FITC-APTS:

[0042] FITC-APTS can be prepared by adding FITC (0.1 g) to 5 ml APTS in a capped tube or bottle, shaking and mixing for 2 hours at room temperature in the dark;

[0043] 3) Synthesis of R6G-FITC-MSN:

[0044] Mix 100 microliters of R6G-APTS and 100 microliters of FITC-APTS with 2.3 ml of TEOS (tetraethyl orthosilicate), then add 240 ml of deionized water, 0.5 g of CTAB (cetyltrimethylammonium bromide), 1.75ml of sodium hydroxide ( 2 M) in a clean Erlenmeyer flask, adjust the temperature of the Erlenmeyer flask to 80 degrees Celsius through a water bath, and shake at this temperature for 2 hours to mix, and then centri...

Embodiment 2

[0045] Example 2: Ratiometric pH titration of R6G-FITC-MSN under dual-wavelength light excitation

[0046] Sodium phosphate buffers with different pH gradients were used to prepare R6G-FITC-MSN into a solution with a final concentration of 1 μg / ml, and the excitation spectra of fluorescein excited at 488nm and rhodamine 6G lactam excited at 533nm corresponding to different pHs were measured. spectrum.

[0047] Such as figure 2 As shown in Figure C in the figure: the curve formed by connecting the hollow points in the figure is the acidity titration curve of the fluorescence emission intensity ratio of R6G-FITC-MSNs under dual-wavelength excitation (the excitation wavelength of FITC is 488nm, and the excitation wavelength of R6G-amide at 530 nm). The fluorescence excited by rhodamine 6G lactam increased with the decrease of pH at different pH, while the fluorescence of fluorescein excited by 488nm decreased with the decrease of pH. The fluorescence of the two responds dif...

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Abstract

The invention discloses a mesoporous silicon dioxide nano particle with a bifluorescence mark, which is a mesoporous silicon dioxide nano particle formed by loading fluorescein isothiocyanate and rhodamine 6G containing a molecular lactam ring together. The invention also discloses a preparation method of the mesoporous silicon dioxide nano particle with the bifluorescence mark and application of the mesoporous silicon dioxide nano particle in detecting a pH value in cell lysosome. The mesoporous silicon dioxide nano particle containing an alkali sensitive FITC (Fluorescein Isothiocyanate) fluorescence molecule and an acid sensitive rhodamine-lactam molecule is used for detecting the pH of the lysosome by a ratio method and has a wide application prospect in the aspect of early diagnosis of diseases, such as cystic fibrosis, asthma, and the like.

Description

technical field [0001] The invention relates to mesoporous silicon dioxide nanoparticles, in particular to double fluorescent-labeled mesoporous silicon dioxide nanoparticles containing acid-sensitive rhodamine 6G derivatives with molecular lactam rings and fluorescein isothiocyanate. Background technique [0002] The pH distribution in eukaryotic cells is characteristically related to organelles, for example, the cytoplasm is nearly neutral (pH 7.2) while the lysosome is acidic (pH 6.0-4.0). The acidic environment in lysosomes is crucial to biological processes such as endocytosis, autophagy, and apoptosis of eukaryotic cells. The acidic changes in lysosomes can be observed in processes such as cell maturation and cancer cell proliferation. Therefore, detecting the pH in lysosomes is of great significance for scientific research. The single fluorescence intensity method based on a single luminophore has many shortcomings in practical applications, such as large experimenta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06G01N21/64B82Y15/00B82Y20/00
Inventor 韩家淮韩守法李柱吴淑琪
Owner XIAMEN UNIV
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